An<i>in vitro</i>assessment of growth promoting activity of a synthetic basic fibroblast growth factor (b-FGF) using Rama-27 cell line

Bazzaz, Ayoub A.; Bukhari, Fatin O.; Mohammed, Amal I.; Chelebi, Noorhan A.

doi:10.1016/j.jtusci.2012.10.003

Cited by 1 publication

(2 citation statements)

References 28 publications

(32 reference statements)

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“…confluence (2x weekly) to allow for variable rates of growth while the cells were passaged at 2 concentrations (1:4 and 1:8) according to Bazzaz, et al, [1] methodology. Freshly prepared PBS solution with pH 7.2 was prepared and adjusted prior each experiment.…”

Section: Passaging Cells and The Passaging Proceduresmentioning

confidence: 99%

“…An explosion of discoveries in the field of cell culture and growth factors has already invaded many fields of biology, including direct clinical application as well as the most modern fundamental scientific problems in medicine. Generally, GFs are a large group of polypeptides, which induce cell multiplication both in vitro and in vivo [1,2] . They do not share any common structural features but could well be grouped into families of molecules with related sequences and structures [3] .…”

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confidence: 99%

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Evaluation of the Growth Promoting Potency of 6 Different Synthetic Peptides Using Rama-27 Cell-Line

Chelebi¹

2017

WJPR

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Six versions of chemically synthesized peptides similar to milk peptides that have nutriceutical, therapeutic and cosmetic (anti-aging) properties were prepared by peptide synthesizer in the laboratory and subjected to bioassay method involved rat mammary gland cells (Rama-27) in vitro. Both molecular weights and sequences of these peptides were confirmed using Positive Ion Electroscopy Mass Spectrometry analysis by Pepsyn's standard operating procedure for peptide synthesis. Cell culture techniques with doses ranging from (3.125ng/mL-450μg/mL) of these peptides dissolved in normal phosphate buffer saline (PBS) of pH 7.2 showed, alone, an insignificant effects as a growth promoting factor onto propagation of Rama-27 cells. Various doses of standard natural product (SNP), the extract of cow whey, alone, or mixed with equal volume of the synthesized peptides produced significantly higher growth promoting effects at doses 25-50 μg/mL. These results suggest that synthesized peptides are, alone, inactive and do lack the key co-factors as complementary elements to stimulate cell proliferation in vitro, unless mixed with other elements i.e. fetal calf serum (FCS) and SNP or alkaline buffer to activate or promote their growth activity characteristics.It is concluded that the composition of these peptides does still lack the natural key element to possess a full potency as a growth factor.

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Section: Passaging Cells and The Passaging Proceduresmentioning

confidence: 99%

mentioning

confidence: 99%