An <i>in vitro</i> assessment of the interaction of cadmium selenide quantum dots with DNA, iron, and blood platelets

Dunpall, Rekha; Nejo, Adeola A.; Pullabhotla, Viswanadha Srirama Rajasekhar; Opoku, Andy R.; Revaprasadu, Neerish; Shonhai, Addmore

doi:10.1002/iub.1100

Cited by 24 publications

(15 citation statements)

References 22 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results corroborate with other published results for similar sized QDs, where 2.6 and 4.8 nm CdTe QDs induced minimal platelet aggregation in PRP (Samuel et al, ). However, these results differ from other platelet aggregation studies which used QDs synthesised from other materials (Dunpall et al, ; Geys et al, ; Ramot et al, ). These results suggest that nanoparticle material, as well as size, may play a role in the interaction of QDs and haemostasis.…”

Section: Resultscontrasting

confidence: 94%

The anticoagulant properties of cadmium telluride quantum dots

Maguire

Lavin

Doyle

et al. 2018

J of Interdiscip Nanomed

View full text Add to dashboard Cite

The size‐dependent optical properties of quantum dots (QDs) are frequently exploited for use in medical imaging and labelling applications. Similarly, presented here, they also elicit profound size‐dependent anticoagulant properties. Cadmium telluride quantum dot (QDs) (3.2 nm) were shown to have a dramatic anticoagulant effect centred on around the intrinsic coagulation pathway, compared to their 3.6 nm counterparts. Several clinically relevant diagnostic tests were carried out over a concentration range of the QDs and demonstrated that the 3.2 nm QDs elicited their response on the intrinsic pathway as a whole, yet the activity of the individual intrinsic coagulation factors was not affected. The mechanism appears also to be strongly influenced by the concentration of calcium ions and not cadmium ions leached from the QDs. Static and shear‐based primary haemostasis assays were also carried out, demonstrating a profound anticoagulant effect which was independent of platelets and phospholipids. The data presented here suggest that the physical–chemical properties of the QDs may have a role in the modulation of haemostasis and the coagulation cascade, in a yet not fully understood mechanism. This study has implications for the use of similar QDs as diagnostic or therapeutic tools in vivo, and for the occupational health and safety of those working with such materials.

show abstract

Section: Resultscontrasting

confidence: 94%

The anticoagulant properties of cadmium telluride quantum dots

Maguire

Lavin

Doyle

et al. 2018

J of Interdiscip Nanomed

View full text Add to dashboard Cite

show abstract

“…This leads to the suggestion that the carboxyl QDs activate the coagulation cascade through the intrinsic pathway, since the thrombotic effect was not detected following pre-treatment of mice with heparin. These results correlate well with the reports that CdSe QDs can induce platelet aggregation in vitro [115,116]. The dramatic effects of different nanoparticle types, including QDs, on the coagulation cascade are bound to develop as a hot topic in the focus of attention of research groups worldwide.…”

Section: Unresolved Problems Challenges and Controversiessupporting

confidence: 89%

Quantum dots in nanomedicine: recent trends, advances and unresolved issues

Volkov

2015

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

a b s t r a c tThe review addresses the current state of progress in the use of ultra-small nanoparticles from the category of quantum dots (QDs), which presently embraces a widening range of nanomaterials of different nature, including "classical" semiconductor groups III-V and II-VI nanocrystals, along with more recently emerged carbon, silicon, gold and other types of nanoparticles falling into this class of nanomaterials due to their similar physical characteristics such as small size and associated quantum confinement effects. A diverse range of QDs applications in nanomedicine has been extensively summarised previously in numerous publications. Therefore, this review is not intended to provide an all-embracing survey of the well documented QDs uses, but is rather focused on the most recent emerging developments, concepts and outstanding unresolved problematic and sometimes controversial issues. Over 125 publications are overviewed and discussed here in the context of major nanomedicine domains, i.e. medical imaging, diagnostics, therapeutic applications and combination of them in multifunctional theranostic systems.© 2015 Elsevier Inc. All rights reserved. Quantum dots in nanomedicine: recent trendsWithin a steadily increasing database of diverse nanomaterials reported as suitable for applications in nanomedicine [1e10], quantum dots (QDs) deservedly occupy a special niche as nanoparticles with a unique track record full of high expectations, dramatic pitfalls and often controversial experimental evidence. From the early optimistic aspirations of them as breakthrough tools for multipurpose in vitro and in vivo applications [11e17], they have been subject to a period of partial relinquishment following the sombre realisation that in the original unmodified state QDs did not stand a chance to deserve a unanimous approval as imaging or drug delivery vehicles in humans, due to their intrinsic toxicity and lack of biodegradability perspective. The disappointingly low yield of exploitable outputs following the initial rounds of heavy investments in the field has also contributed to the overall decline in research productivity metrics. The analysis of the recent ten years' trends in publications number in this area related to the QDs medical applications in vivo in general (Fig. 1 A), as well as for imaging and diagnostics purposes (Fig. 1 B, C) according to Thomson Reuters Web of Science™ database clearly reflects such "cooling down" period after 2010 resulting in a significant reduction in the initially steady exponential growth of publication rates. This trend has been partially or completely reversed by 2014 and it is intriguing whether we will see it sustained in 2015 and beyond.Such returning enthusiasm has been reinvigorated by a number of objective tendencies in the related technological developments, including the arrival of innovative nanotechnology-enabled tools for diagnostic and ex vivo imaging applications [18,19], the arrival of new types of QDs of alternative nature offering the opportunities of r...

show abstract

“…The complete plasma proteome is composed of approximately 3700 different proteins and about 50 of these have been shown to associate with nanoparticles (6,7). Therefore, there are concerns regarding the safety of nanoparticles and whether modifications that biological materials undergo upon their interaction with nanoparticles may alter their intended function (8).…”

Section: Introductionmentioning

confidence: 99%

Cysteine‐capped gold nanoparticles suppress aggregation of proteins exposed to heat stress

et al. 2013

Self Cite

View full text Add to dashboard Cite

Gold nanoparticles show a lot of promise as potential agents for drug delivery and disease diagnosis. Because of this, it is important that the interaction between gold nanoparticles and biomolecules be well characterized to avoid undesirable consequences. In this study, gold nanoparticles were synthesized by the reduction of gold salt by sodium borohydride in the presence of cysteine as the capping agent. The physical features of the nanoparticles were analyzed using Ultraviolet-Visible spectrophotometry and transmission electron microscopy. The interaction between gold nanoparticles and the following proteins: bovine serum albumin, citrate synthase, malate dehydrogenase, and human heat shock protein 70 was investigated by UV-Vis spectrophotometry. The stability of the proteins against heat stress was assessed by monitoring their aggregation at 48 8C, either in the presence or absence of gold nanoparticles. The gold nanoparticles were capable of suppressing the heat-induced aggregation of the proteins. Furthermore, apart from possessing independent protein-aggregation suppression function, the AuNPs also augmented the chaperone function of human heat shock protein 70. Findings from this study demonstrate that cyteinecoated gold nanoparticles exhibit chaperone-like activity and have the capability to stabilize proteins to which they may be conjugated. V C 2013 IUBMB Life, 65(5): 454-461, 2013.

show abstract

An in vitro assessment of the interaction of cadmium selenide quantum dots with DNA, iron, and blood platelets

Cited by 24 publications

References 22 publications

The anticoagulant properties of cadmium telluride quantum dots

The anticoagulant properties of cadmium telluride quantum dots

Quantum dots in nanomedicine: recent trends, advances and unresolved issues

Cysteine‐capped gold nanoparticles suppress aggregation of proteins exposed to heat stress

Contact Info

Product

Resources

About