AnIn VitroandIn VivoStudy of the α-Amylase Activity of Phaseolamin

Gouveia, Neire Moura de; Alves, Fernanda Vieira; Furtado, Fabiana Barcelos; Scherer, Danielli Luana; Mundim, Antônio Vicente; Espindola, Foued Salmen

doi:10.1089/jmf.2013.0044

Cited by 18 publications

(13 citation statements)

References 33 publications

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“…Animals were fasted for another 90 min after the injection. Ten days after the STZ injection, rats with a fasting blood glucose level greater than 250 mg/dL were used for subsequent experiments [ 16 , 17 ]. Body weight and blood glucose levels were monitored in animals undergoing the different treatments (water, glibenclamide, and V .…”

Section: Methodsmentioning

confidence: 99%

Phytochemical characterization of the Vochysia rufa (Vochysiaceae) extract and its effects on oxidative stress in the pancreata of streptozotocin-induced diabetic rats

et al. 2017

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Aqueous extract of macerated Vochysia rufa stem bark has been commonly used in the treatment of diabetes. Therefore, we evaluated the antihyperglycemic and antioxidant effects of an extract of V. rufa on the pancreata of streptozotocin (STZ)-induced diabetic rats. Animals received one of the following treatments daily by oral gavage: water (diabetic-control), V. rufa extract (diabetic-V. rufa), or glibenclamide (diabetic-GBD). Total antioxidant capacity; levels of thiobarbituric acid reactive substances, reduced glutathione, and sulfhydryls; and superoxide dismutase, catalase, and glutathione peroxidase (GPx) activities were measured in the pancreas. Biochemical analysis of serum total cholesterol and fractions, triglycerides, creatinine, urea, acid uric, ALP, γ-GT, AST, and ALT was performed, and pancreatic β-cells positive for insulin were evaluated by immunohistochemistry. Rats treated with extract exhibited a decrease in fasting blood glucose compared with levels in diabetic control rats. GPx activity and sulfhydryl levels were significantly lower in diabetic-V. rufa rats compared with those of diabetic-control rats. V. rufa extract acted to normalize the biochemical alterations found in diabetic rats (diabetic-controls), as demonstrated by increases in urea, HDL, ALP, AST, and ALT. Reduction in blood glucose was independent of an increase in insulin. The V. rufa extract was found to be composed of free sugars (inositol, galactose, glucose, mannose, sucrose, arabinose, and ribose) as the main metabolites. Thus, aqueous extract of the stem bark of V. rufa is capable of reducing blood glucose, resulting in an antioxidant effect on the pancreatic tissue of STZ-diabetic rats.

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Section: Methodsmentioning

confidence: 99%

Phytochemical characterization of the Vochysia rufa (Vochysiaceae) extract and its effects on oxidative stress in the pancreata of streptozotocin-induced diabetic rats

et al. 2017

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“…The active polyphenols present in this extract impart a blood glucose-lowering effect via insulin sensitization as well as inhibition of α-amylase and α-glucosidase activities (Ramachandran and Rajasekaran, 2014). Commercial phaseolamin, which has high α-amylase inhibitory ability, can reduce the blood glucose levels in streptozotocin-induced diabetic rats; therefore, it possesses therapeutic potential for the treatment or prevention of the complications of diabetes (De Gouveia et al, 2014). According to these results, substances with the ability to inhibit the activity of α-amylase and/or α-glucosidase may also possess anti-hyperglycemic potential via alleviation of the high blood glucose status in diabetic individuals.…”

Section: Introductionmentioning

confidence: 99%

Optimization Extraction Conditions with Ultrasound for Anti-hyperglycemic Activities from Psidium guajava Leaf

Liu

Wang

Huang³

et al. 2015

FSTR

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The present study evaluated the optimal parameters for Psidium guajava leaf extraction by ultrasound to obtain maximal anti-hyperglycemic activities in the extract. The response surface methodology was employed for empirical model building. The maximum inhibition of the two starch-digesting enzymes α-amylase and α-glucosidase was determined. The optimized extraction parameters of solvent-to-solid ratio, extraction time, and extraction temperature for obtaining the maximum inhibitory activity for 50 µg/mL extract were determined to be the following: 12.07 (v/w), 5.22 min, and 59.77℃ for α-amylase (47.23% ± 0.76%) and 12 (v/w), 5.22 min, and 59.94℃ for α-glucosidase (59.42% ± 0.65%), respectively. The F values for the lack-of-fit were not significant ( p > 0.05) for both second-order models, indicating them to be appropriate for describing the response surface. In conclusion, our study presents the optimal conditions for ultrasound-assisted extraction of guava leaf as a potential substance for use in diabetes treatment.

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“…De Gouveia et al found that patients treated with phaseolamin did not have any decrease in body weight, but this study was conducted for only 20 days and therefore, follow-up bias could have affected the conclusions of this study. 99 The use of phaseolamine as an antihyperglycemic is contended. Oliveira et al found that phaseolamin can be used to control diabetes due to its anti-hyperglycemic effect.…”

Section: Bacterial Amylasementioning

confidence: 99%