“…Although palate organ cultures enable various experiments which are impossible in utero, they sometimes produce conflicting results. Palate culture methods reported thus far can be largely divided into the static culture method using a CO 2 incubator (Pourtois, 1966(Pourtois, , 1968Smiley and Koch, 1971, 1972, 1975Dixon and Ferguson, 1992;Griffith and Hay, 1992;Martínez-Álvarez et al, 2000;Cuervo et al, 2002;Cuervo and Covarrubias, 2004) and the suspension culture method with rolling bottles (Shiota et al, 1990;Brown et al, 2002). In those cultures, some fundamental questions remain such as: (1) In order from the left, oral and medial views of the single shelf explants, and the cell morphology of the medial edge and oral and nasal epithelia, respectively.…”