An <i>in vitro</i> and <i>in vivo</i> evaluation of the virulence of egg drop syndrome virus for the chicken reproductive tract

Raj, G. Dhinakar; Sivakumar, S.; Manohar, B. Murali; Nachimuthu, K.; Nainar, A. Mahalinga

doi:10.1080/03079450020023140

Cited by 9 publications

(3 citation statements)

References 17 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Precociously induced oviducts by oestrogen treatment of young chickens have been used successfully as a model system for the study of virulence of infectious bronchitis virus (Dhinakar Raj and Jones 1996), avian pneumovirus (Khehra and Jones, 1999), egg drop syndrome virus (Dhinakar Raj et al, 2001) and NDV (Sreenivasa Rao et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Protection of the Reproductive Tract of Young Chicks by Newcastle Disease Virus-induced Haemagglutinationinhibition Antibodies

Raghul

Raj²,

Manohar

et al. 2006

Vet Res Commun

View full text Add to dashboard Cite

The present study was conducted to assess the haemagglutination-inhibition (HI) titres required to protect the chicken reproductive tract against direct damage caused by Newcastle disease virus (NDV). Precociously induced oviduct and uterus by oestrogen treatment of young chicks were used to assess the damage or protection against the damage by analysis of ciliostasis or histopathological lesions. Unvaccinated day-old female white leghorn chickens were used as the maternally derived antibody (MDA) group. Chickens were vaccinated with either a live lentogenic vaccine on day 14 of age or, along with it, an inactivated vaccine at day 36 of age, to generate birds with a range of primary or secondary response induced HI antibodies. Birds with different HI antibody levels were challenged with virulent NDV. It was found that a HI antibody titre of 128 and above was protective against direct damage of the reproductive tract, while the 32-64 titre range was protective when derived through secondary vaccination only.

show abstract

Section: Introductionmentioning

confidence: 99%

Protection of the Reproductive Tract of Young Chicks by Newcastle Disease Virus-induced Haemagglutinationinhibition Antibodies

Raghul

Raj²,

Manohar

et al. 2006

Vet Res Commun

View full text Add to dashboard Cite

show abstract

“…in OOC(u), which was dramatic in contrast to the time taken for ciliostasis following infection with EDS 76 virus (4 to 5 days; Dhinakar Raj et al, 2001), APV (2 days; Khehra & Jones, 1998), and IBV (2 to 3 days; Dhinakar Raj & Jones, 1996). Ciliostasis is a result of NDV replication in the ciliated lining epithelium causing subsequent damage.…”

Section: Discussionmentioning

confidence: 94%

An in vitro and in vivo evaluation of the virulence of Newcastle disease virus and vaccines for the chicken reproductive tract

2002

View full text Add to dashboard Cite

The virulence of two vaccine strains and two field strains of Newcastle disease virus (NDV) for the female reproductive tract of chickens was assessed using oviduct organ cultures (OOC) prepared from precociously-induced oviducts in young chicks by oestrogen treatment. Ciliostasis, haemagglutination and virus isolation from infected OOC supernatants, histopathology and immunoperoxidase test results indicated the pathogenic nature of both vaccine and virulent NDVs for the precocious oviducts. The virulent viruses, mesogenic and lentogenic vaccines caused damage in that order of magnitude and the uterus had a higher susceptibility than oviducts. One virulent and the mesogenic strain of NDV were used for in vivo trials. The pathogenicity was assessed in oestrogen-treated infected chickens using histopathology and immunoperoxidase test. The vaccine virus produced transient damage up to 6 days post-infection, while the damage with the virulent isolate persisted for at least 9 days post-infection. This technique could be a pointer to possible variations in virulence of NDV vaccine and field strains, and warrants further investigation. The potential value of OOC from young chickens for testing the possibility of NDV vaccines causing damage by themselves and offering protection against damage of the reproductive tract caused by virulent isolates is emphasized.

show abstract

“…Recently, PCR of the hexon gene followed by restriction fragment analysis revealed no differences among five Indian isolates of EDS. However, sequence analysis showed that one of them differed by about 5% from the others, illustrating that sequence analysis may be essential in some instances to obtain an accurate epidemiological picture (Dhinaker Raj et al, 2001).…”

Section: Avian Adenovirusesmentioning

confidence: 99%

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Cavanagh¹

2001

Avian Pathology

View full text Add to dashboard Cite

Polymerase chain reaction (PCR)-based approaches to the detection, differentiation and characterization of avian pathogens continue to be developed and refined. The PCRs, or reverse transcriptasePCRs, may be general, designed to detect all or most variants of a pathogen, or to be serotype, genotype or pathotype specific. Progress is being made with respect to making nucleic acid approaches more suitable for use in diagnostic laboratories. Robotic workstations are now available for extraction of nucleic acid from many samples in a short time, for routine diagnosis. Following general PCR, the DNA products are commonly analyzed by restriction endonuclease mapping (restriction fragment length polymorphism), using a small number of restriction endonucleases, based on a large body of sequence data. Increasingly, however, nucleotide sequencing is being used to analyze the DNA product, in part due to the expanding use of non-radioactive sequencing methods that are safe and enable high throughout. In this review, I highlight some recent developments with many avian viruses: Newcastle disease virus; circoviruses in canary and pigeon; infectious bursal disease virus (Gumboro disease virus); avian adenoviruses, including Angara disease/infectious hydropericardium virus, haemorrhagic enteritis virus of turkeys, and egg drop syndrome virus; avian herpesviruses, including infectious laryngotracheitis virus, duck plague virus, psittacine herpesvirus (Pacheco's parrot disease virus), Marek's disease virus and herpesvirus of turkeys; avian leukosis virus (associated with lymphoid leukosis or myeloid leukosis, and egg transmission); avian pneumoviruses (turkey rhinotracheitis virus); avian coronaviruses, including infectious bronchitis virus, turkey coronavirus and pheasant coronavirus; astrovirus, in the context of poult enteritis and mortality syndrome, and avian nephritis virus; and avian encephalomyelitis virus, a picornavirus related to hepatitis A virus.

show abstract

An in vitro and in vivo evaluation of the virulence of egg drop syndrome virus for the chicken reproductive tract

Cited by 9 publications

References 17 publications

Protection of the Reproductive Tract of Young Chicks by Newcastle Disease Virus-induced Haemagglutinationinhibition Antibodies

Protection of the Reproductive Tract of Young Chicks by Newcastle Disease Virus-induced Haemagglutinationinhibition Antibodies

An in vitro and in vivo evaluation of the virulence of Newcastle disease virus and vaccines for the chicken reproductive tract

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Contact Info

Product

Resources

About