An
                    <i>in vitro</i>
                    3D model using collagen coated gelatin nanofibers for studying breast cancer metastasis

Janani, G.; Pillai, Mamatha M.; Selvakumar, R.; Bhattacharyya, Amitava; Sabarinath, C

doi:10.1088/1758-5090/aa5510

Cited by 31 publications

(15 citation statements)

References 34 publications

(46 reference statements)

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“…To this end, an in vitro 3D model was constructed using collagen-coated gelatin nanofibers to investigate the metastasis of breast cancer. 984 This tumor model mimicked the extracellular composition, surface complexity, and mechanical properties of connective tissues, achieving an obvious cellular invasion. In addition, the cellular microenvironment, in particular the component of ECM, was found to play an important role in regulating the metastasis of cancer cells.…”

Section: Electrospun Nanofibers For Biomedical Applicationsmentioning

confidence: 99%

Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications

et al. 2019

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Electrospinning is a versatile and viable technique for generating ultrathin fibers. Remarkable progress has been made with regard to the development of electrospinning methods and engineering of electrospun nanofibers to suit or enable various applications. We aim to provide a comprehensive overview of electrospinning, including the principle, methods, materials, and applications. We begin with a brief introduction to the early history of electrospinning, followed by discussion of its principle and typical apparatus. We then discuss its renaissance over the past two decades as a powerful technology for the production of nanofibers with diversified compositions, structures, and properties. Afterward, we discuss the applications of electrospun nanofibers, including their use as “smart” mats, filtration membranes, catalytic supports, energy harvesting/conversion/storage components, and photonic and electronic devices, as well as biomedical scaffolds. We highlight the most relevant and recent advances related to the applications of electrospun nanofibers by focusing on the most representative examples. We also offer perspectives on the challenges, opportunities, and new directions for future development. At the end, we discuss approaches to the scale-up production of electrospun nanofibers and briefly discuss various types of commercial products based on electrospun nanofibers that have found widespread use in our everyday life.

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Section: Electrospun Nanofibers For Biomedical Applicationsmentioning

confidence: 99%

Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications

et al. 2019

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“…Because of their relatively high surface charge characteristics, some proteins have been shown to be particularly suitable for the formation of nanoparticles using this method, including soy protein (Vega‐Lugo & Lim, ; Zhao et al., ) and gelatin (Mindru, Mindru, Malutant, & Tura, ; Okutan, Terzi, & Altay, ). Globular proteins cannot be electrospun into fibers in their native globular state and the electro‐spinning properties of polymers can be further enhanced by the addition of other polymers, such as polyethylene oxide and polyvinyl alcohol (Tang, Ozcam, Stout, & Khan, ; Vega‐Lugo & Lim, ; Woerdeman, Shenoy, & Breger, ) as a synthetic and gelatin (Janani, Pillai, Selvakumar, Bhattacharyya, & Sabarinath, ; Lu, Xu, Zhang, Ma, & Guo, ) as a natural aiding agents.…”

Section: Production Methods and Modificationmentioning

confidence: 99%

Protein‐Based Delivery Systems for the Nanoencapsulation of Food Ingredients

Fathi

Donsı̀

McClements

2018

Comp Rev Food Sci Food Safe

189

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Many proteins possess functional attributes that make them suitable for the encapsulation of bioactive agents, such as nutraceuticals and pharmaceuticals. This article reviews the state of the art of protein-based nanoencapsulation approaches. The physicochemical principles underlying the major techniques for the fabrication of nanoparticles, nanogels, and nanofibers from animal, botanical, and recombinant proteins are described. Protein modification approaches that can be used to extend their functionality in these nanocarrier systems are also described, including chemical, physical, and enzymatic treatments. The encapsulation, retention, protection, and release of bioactive agents in different protein-based nanocarriers are discussed. Finally, some of the major challenges in the design and fabrication of protein-based delivery systems are highlighted.

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“…[ 65,66 ] In top‐down approaches, cells are seeded on top of a preformed scaffold. [ 63 ] Among them, fibers, [ 67,68 ] foams, [ 69 ] porous/solid microparticles [ 70–72 ] and decellularized cell‐derived and tissues/organs‐derived matrix scaffolds [ 73,74 ] have been widely used. The main limitation of these scaffolds is related to the necessity of cells infiltration into the structure, an important technical aspect, that generally leads to difficulties in guaranteeing a homogeneous cell distribution and infiltration throughout the whole structure.…”

Section: D In Vitro Models To Recapitulate the Tmementioning

confidence: 99%

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

et al. 2021

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The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date, ECM‐mimetic hydrogels have been widely explored for 3D in vitro disease modeling owing to their bioactive properties that can be further adapted to the biochemical and biophysical properties of native tumors. Gathering on this momentum, herein the current landscape of intrinsically bioactive protein and peptide hydrogels that have been employed for 3D tumor modeling are discussed. Initially, the importance of recreating such microenvironment and the main considerations for generating ECM‐mimetic 3D hydrogel in vitro tumor models are showcased. A comprehensive discussion focusing protein, peptide, or hybrid ECM‐mimetic platforms employed for modeling cancer cells/stroma cross‐talk and for the preclinical evaluation of candidate anticancer therapies is also provided. Further development of tumor‐tunable, proteinaceous or peptide 3D microtesting platforms with microenvironment‐specific biophysical and biomolecular cues will contribute to better mimic the in vivo scenario, and improve the predictability of preclinical screening of generalized or personalized therapeutics.

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An in vitro 3D model using collagen coated gelatin nanofibers for studying breast cancer metastasis

Cited by 31 publications

References 34 publications

Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications

Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications

Protein‐Based Delivery Systems for the Nanoencapsulation of Food Ingredients

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

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