Anin silico,in vitroandin vivoinvestigation of indole-3-carboxaldehyde identified from the seawater bacteriumMarinomonassp. as an anti-biofilm agent againstVibrio choleraeO1

Rajalaxmi, Murugan; Shafreen, Rajamohamed Beema; Iyer, Prasanth Mani; Vino, Raja Sahaya; Balamurugan, Krishnaswamy; Pandian, Shunmugiah Karutha

doi:10.1080/08927014.2016.1154545

Cited by 18 publications

(12 citation statements)

References 40 publications

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“…Further, the natural product Indole-3-carboxaldehyde biosynthesized by Marinomonas sp. was recently described as an anti-biofilm agent against V. cholerae O1 ( Rajalaxmi et al, 2016 ). The molecule(s) responsible for the here observed activity will be subject of future studies.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential of Bacteria Associated with Marine Sea Slugs from North Sulawesi, Indonesia

et al. 2017

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Nudibranchia, marine soft-bodied organisms, developed, due to the absence of a protective shell, different strategies to protect themselves against putative predators and fouling organisms. One strategy is to use chemical weapons to distract predators, as well as pathogenic microorganisms. Hence, these gastropods take advantage of the incorporation of chemical molecules. Thereby the original source of these natural products varies; it might be the food source, de novo synthesis from the sea slug, or biosynthesis by associated bacteria. These bioactive molecules applied by the slugs can become important drug leads for future medicinal drugs. To test the potential of the associated bacteria, the latter were isolated from their hosts, brought into culture and extracts were prepared and tested for antimicrobial activities. From 49 isolated bacterial strains 35 showed antibiotic activity. The most promising extracts were chosen for further testing against relevant pathogens. In that way three strains showing activity against methicillin resistant Staphylococcus aureus and one strain with activity against enterohemorrhagic Escherichia coli, respectively, were identified. The obtained results indicate that the sea slug associated microbiome is a promising source for bacterial strains, which hold the potential for the biotechnological production of antibiotics.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential of Bacteria Associated with Marine Sea Slugs from North Sulawesi, Indonesia

et al. 2017

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“…The bioactive fractions were extracted by polarity based solvent extraction from non-polar to polar manner (n-hexane < benzene < petroleum ether < dichloromethane < chloroform < ethyl acetate < methanol) ( Rajalaxmi et al, 2016 ). SCME was subjected to column chromatography using silica gel (60–120 mesh size, 50 cm × 2 cm) (Merck, United States).…”

Section: Methodsmentioning

confidence: 99%

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

et al. 2018

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In recent decades, fungal infections have incredibly increased with Candida genus as the major cause of morbidity and mortality in hospitalized and immunocompromised patients. Most of the Candida species are proficient in biofilm formation on implanted medical devices as well as human tissues. Biofilm related Candida infections are very difficult to treat using common antifungal agents owing to their increased drug resistance. To address these issues, the present study investigated the antibiofilm and antivirulent properties of Syzygium cumini derived quinic acid in combination with known antifungal compound undecanoic acid. Initially, antibiofilm potential of S. cumini leaf extract was assessed and the active principles were identified through gas chromatography and mass spectrometry analysis. Among the compounds identified, quinic acid was one of the major compounds. The interaction between quinic acid and undecanoic acid was found to be synergistic in the Fractional inhibitory concentration index (≤0.5). Results of in vitro assays and gene expression analysis suggested that the synergistic combinations of quinic acid and undecanoic acid significantly inhibited virulence traits of Candida spp. such as the biofilm formation, yeast-to-hyphal transition, extracellular polymeric substances production, filamentation, secreted hydrolases production and ergosterol biosynthesis. In addition, result of in vivo studies using Caenorhabditis elegans demonstrated the non-toxic nature of QA-UDA combination and antivirulence effect against Candida spp. For the first time, synergistic antivirulence ability of quinic acid and undecanoic acid was explored against Candida spp. Thus, results obtained from the present study suggest that combination of phytochemicals might be used an alternate therapeutic strategy for the prevention and treatment of biofilm associated Candida infection.

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“…Nicotinamide reportedly enhances growth of both Gram-negative and Gram-positive bacteria, such as M. avium , Propionibacterium acnes , S. aureus , and B. macerans [ 73 , 74 , 75 , 76 ]. Indole-3-carboxaldehyde was shown to efficiently inhibit biofilm formation by Vibrio cholerae O1 [ 77 ]. The utilization of urocanic acid by Pseudomonas and Aeromonas strains has been reported [ 78 , 79 ].…”

Section: Resultsmentioning

confidence: 99%

Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation

2021

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The cultivation of the cellulolytic bacterium, Clostridium thermocellum, can have cost-effective cellulosic biomass utilizations, such as consolidated bioprocessing, simultaneous biological enzyme production and saccharification. However, these processes require a longer cultivation term of approximately 1 week. We demonstrate that constituents of the C. thermocellum membrane vesicle fraction significantly promoted the growth rate of C. thermocellum. Similarly, cell-free Bacillus subtilis broth was able to increase C. thermocellum growth rate, while several B. subtilis single-gene deletion mutants, e.g., yxeJ, yxeH, ahpC, yxdK, iolF, decreased the growth stimulation ability. Metabolome analysis revealed signal compounds for cell–cell communication in the C. thermocellum membrane vesicle fraction (ethyl 2-decenoate, ethyl 4-decenoate, and 2-dodecenoic acid) and B. subtilis broth (nicotinamide, indole-3-carboxaldehyde, urocanic acid, nopaline, and 6-paradol). These findings suggest that the constituents in membrane vesicles from C. thermocellum and B. subtilis could promote C. thermocellum growth, leading to improved efficiency of cellulosic biomass utilization.

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Anin silico,in vitroandin vivoinvestigation of indole-3-carboxaldehyde identified from the seawater bacteriumMarinomonassp. as an anti-biofilm agent againstVibrio choleraeO1

Cited by 18 publications

References 40 publications

Antimicrobial Potential of Bacteria Associated with Marine Sea Slugs from North Sulawesi, Indonesia

Antimicrobial Potential of Bacteria Associated with Marine Sea Slugs from North Sulawesi, Indonesia

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation

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