An<i>Acetobacterium</i>strain isolated with metallic iron as electron donor enhances iron corrosion by a similar mechanism as<i>Sporomusa sphaeroides</i>

Philips, Jo; Monballyu, Eva; Georg, Steffen; Paepe, Kim De; Prévoteau, Antonin; Rabaey, Korneel; Arends, Jan

doi:10.1093/femsec/fiy222

Cited by 44 publications

(55 citation statements)

References 59 publications

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“…Here we demonstrate, that Baltic-acetogens are most likely using oozed endogenous enzymes for quick retrieval of electrons from Fe 0 . This was similar to earlier studies, which investigated pure culture acetogens corroding Fe 0 , such as Sporomusa or Acetobacterium strains [35,54].…”

Section: Resultssupporting

confidence: 90%

“…Acetogens have been credited to use a variety of mechanisms for electron uptake from Fe 0 including uptake of abiotic H 2 [54], uptake of enzymatically evolved H 2 [35,55] or direct-electron uptake. The later is a possibility inferred from the property of some acetogens to grow on electrodes under non-hydrogenogenotrophic conditions [50, 56, 57].…”

Section: Resultsmentioning

confidence: 99%

“…We observed that accumulation of acetate increased by 22% (n=10, p<0.00001) compared to the mixed Baltic community, and by 7% (n=10, p<0.02) compared to Baltic-acetogens alone, after inhibiting their competitors, the methanogens. We conclude that Baltic-acetogens are most likely to use a mechanism of electron uptake from Fe 0 mediated by enzymes similar to other acetogens [35,54]. In the Baltic corrosive community Clostridium -species are likely to play the role of acetogens, since several species have been shown to produce acetate either by electrosynthesis of autotrophically on H 2 [50, 58, 59].…”

Section: Resultsmentioning

confidence: 99%

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BalticMethanosarcinaandClostridiumcompete for electrons from metallic iron

Snoeyenbos-West

Löscher

Thamdrup

et al. 2019

Preprint

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13Microbial induced corrosion of steel structures, used for transport or storage of fuels, 14 chemical weapons or waste radionuclides, is an environmental and economic threat. 15In non-sulfidic environments, the exact role of methanogens in steel corrosion is 16 poorly understood. From the non-sulfidic, methanogenic sediments of the Baltic Sea 17 corrosive communities were enriched using exclusively Fe 0 as electron donor and 18 CO 2 as electron acceptor. Methane and acetate production were persistent for three 19 years of successive transfers. Methanosarcina and Clostridium were attached to the 20 Fe 0 , and dominated metagenome libraries. Since prior reports indicated 21Methanosarcina were merely commensals, consuming the acetate produced by 22 acetogens, we investigated whether these methanogens were capable of Fe 0 corrosion 23 without bacterial partners (inhibited by an antibiotic cocktail). Unassisted, 24 methanogens corroded Fe 0 to Fe 2+ at similar rates to the mixed community. 25 Surprisingly, in the absence of competitive bacteria, Baltic-Methanosarcina produced 26 six times more methane than they did in the mixed community. This signifies that 27Baltic-Methanosarcina achieved better corrosion alone, exclusive of an operative 28 bacterial partner. Our results also show that together with acetogens, Methanosarcina 29 interact competitively to retrieve electrons from Fe 0 rather than as commensals as 30 previously assumed. 31 32

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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BalticMethanosarcinaandClostridiumcompete for electrons from metallic iron

Snoeyenbos-West

Löscher

Thamdrup

et al. 2019

Preprint

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“…malicum and an isolate most closely related to A. wieringae more effectively extract electrons from solid Fe(0) coupons compared to A. woodii [37]. Additionally, A. sp.…”

Section: Many Of the Acetobacterium Genomes/mags Encoded For Portionsmentioning

confidence: 94%

“…The genus appears to have varied metabolic capabilities, including low-temperature growth [32], debromination of polybrominated diphenyl ethers (PBDEs) [33], hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) degradation [34,35], electrode-mediated acetogenesis [36], enhanced iron corrosion [37], and isoprene degradation [38]. To provide a more robust genome dataset, we sequenced and manually curated the genomes of four Acetobacterium strains, including A. malicum and three psychrophilic strains (A.…”

Section: Introductionmentioning

confidence: 99%

The defining genomic and predicted metabolic features of the Acetobacterium genus

Ross

Marshall

Gulliver

et al. 2020

Preprint

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Acetogens are anaerobic bacteria capable of fixing CO 2 or CO to produce acetyl-CoA and ultimately acetate using the Wood-Ljungdahl pathway (WLP). This autotrophic metabolism plays a major role in the global carbon cycle. Acetobacterium woodii, which is a member of the Eubacteriaceae family and type strain of the Acetobacterium genus, has been critical for understanding the biochemistry and energy conservation in acetogens. Other members of the Acetobacterium genus have been isolated from a variety of environments or have had genomes recovered from metagenome data, but no systematic investigation has been done into the unique and varying metabolisms of the genus. Using the 4 sequenced isolates and 5 metagenome-assembled genomes available, we sequenced the genomes of an additional 4 isolates (A. fimetarium, A. malicum, A. paludosum, and A. tundrae) and conducted a comparative genome analysis of 13 different Acetobacterium genomes to obtain better phylogenomic resolution and understand the metabolic diversity of the Acetobacterium genus. Our findings suggest that outside of the reductive acetyl-CoA (Wood-Ljungdahl) pathway, the Acetobacterium genus is more phylogenetically and metabolically diverse than expected, with metabolism of fructose, lactate, and H 2 :CO 2 constant across the genus, and ethanol, methanol, caffeate, and 2,3-butanediol varying across the genus. While the gene arrangement and predicted proteins of the methyl (Cluster II) and carbonyl (Cluster III) branches of the Wood Ljungdahl pathway are highly conserved across all sequenced Acetobacterium genomes, Cluster 1, encoding the formate dehydrogenase, is not. Furthermore, the accessory WLP components, including the Rnf cluster and electron bifurcating hydrogenase, were also well conserved, though all but four strains encode for two Rnf clusters. Additionally, comparative genomics revealed clade-specific potential functional capabilities, such as amino acid transport and metabolism in the psychrophilic group, and biofilm formation in the A. wieringae clade, which may afford these groups an advantage in low-temperature growth or attachment to solid surfaces, respectively. Overall, the data presented herein provides a framework for examining the ecology and evolution of the Acetobacterium genus and highlights the potential of these species as a source of fuels and chemicals from CO 2 -feedstocks.

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Desulfovibrio vulgaris as a model microbe for the study of corrosion under sulfate‐reducing conditions

Ueki

Lovley

2022

mLife

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Corrosion of iron‐containing metals under sulfate‐reducing conditions is an economically important problem. Microbial strains now known as Desulfovibrio vulgaris served as the model microbes in many of the foundational studies that developed existing models for the corrosion of iron‐containing metals under sulfate‐reducing conditions. Proposed mechanisms for corrosion by D. vulgaris include: (1) H2 consumption to accelerate the oxidation of Fe0 coupled to the reduction of protons to H2; (2) production of sulfide that combines with ferrous iron to form iron sulfide coatings that promote H2 production; (3) moribund cells release hydrogenases that catalyze Fe0 oxidation with the production of H2; (4) direct electron transfer from Fe0 to cells; and (5) flavins serving as an electron shuttle for electron transfer between Fe0 and cells. The demonstrated possibility of conducting transcriptomic and proteomic analysis of cells growing on metal surfaces suggests that similar studies on D. vulgaris corrosion biofilms can aid in identifying proteins that play an important role in corrosion. Tools for making targeted gene deletions in D. vulgaris are available for functional genetic studies. These approaches, coupled with instrumentation for the detection of low concentrations of H2, and proven techniques for evaluating putative electron shuttle function, are expected to make it possible to determine which of the proposed mechanisms for D. vulgaris corrosion are most important.

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AnAcetobacteriumstrain isolated with metallic iron as electron donor enhances iron corrosion by a similar mechanism asSporomusa sphaeroides

Cited by 44 publications

References 59 publications

BalticMethanosarcinaandClostridiumcompete for electrons from metallic iron

BalticMethanosarcinaandClostridiumcompete for electrons from metallic iron

The defining genomic and predicted metabolic features of the Acetobacterium genus

Desulfovibrio vulgaris as a model microbe for the study of corrosion under sulfate‐reducing conditions

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