An HPLC method for simultaneous quantitative determination of seven secoiridoid glucosides separated from the roots of Ilex pubescens

Abstract

A simple and specific high-performance liquid chromatographic method has been developed and validated to simultaneously determine seven secoiridoid glucosides for the first time. Three of them were separated from the ethanolic extract of the roots of Ilex pubescens for the first time, namely nuezhenide A, ligusides B and oleonuezhenide. In quantitative analysis, all of the calibration curves showed good linear regression (r > 0.999) within the tested ranges, and the mean recoveries of three different concentrations ranged from 97.6 to 101.2%. The limit of detection and limit of quantification were <4.18 and 11.63 ng mL , respectively. The relative standard deviation for repeatability and the precision of seven analytes were <3.4 and 1.9%, respectively. The established method was successfully applied to simultaneous determination of seven secoiridoid glucosides in 11 batches of samples collected from different habitats in China.

Efficacy and Mechanisms of Oleuropein in Mitigating Diabetes and Diabetes Complications

“…Oleuropein exists in olive leaf extracts, olive oil, and different parts of the olive tree, such as immature olive fruits, leaves, and branches, and the quantities of oleuropein are summarized in Supporting Information Table S1. Apart from olive trees, oleuropein can also be found in other kinds of plants, such as Lauraceae , Syringa (containing approximately 0.49% oleuropein in the whole plant), Ligustrum (containing approximately 2.91% oleuropein in the leaf extracts), Hibiscus , Jasminum (containing 23.3% to 55.1% oleuropein in the Jasmine flower extracts), Ilex pubescens (0.5915 to 10.5971 mg/g in the root), Philyrea latifolia , Fraximus angustifolia , Fraximus excelsior , and Fraximus chinensis .…”