An Herbal Formula LI85008F Inhibits Lipogenesis in 3T3-L1 Adipocytes

Abstract

The present study demonstrates a novel herbal formulation LI85008F inhibiting adipocyte differentiation and potentiates lipolysis in 3T3-L1 mouse adipocytes. LI85008F is formulated by combining extracts of three Indian herbs Moringa oleifera, Murraya koenigii and Curcuma longa. Oil red O staining of 3T3-L1 adipocytes reveals that LI85008F is a synergistic formulation that inhibits adipocyte differentiation in a dose dependent manner and concurrently down regulates the key adipogenic transcription factors Peroxisome Proliferator-Activated Receptor gamma (PPAR) and CCAAT/enhancer binding protein α (C/EBP). LI85008F confers significant reductions in intracellular triglyceride content in a dose dependent manner. Evidence suggests that LI85008F antagonizes PPAR through Ser112 phosphorylation via MAPK/ERK activation. Immunoblot analyses reveal that LI85008F treatment also down regulates the protein expressions of key PPAR responsive gene products such as Adipocyte differentiation related protein (ADRP), CD36, Adipocyte specific binding protein 2 (aP2) and perilipin. In differentiated adipocytes culture, LI85008F treatment results in significantly (p = 0.0169) increased lipolysis as measured by the release of glycerol. LI85008F does not exhibit cytotoxic effect on adipocytes. Taken together, the results suggest that LI85008F inhibits lipogenesis in adipocytes and concurrently antagonizes PPAR? and other lipogenic factors and in addition, potentiates triglyceride mobilization from the fat cells or enhances lipolysis

Efficacy and tolerability of Meratrim for weight management: a randomized, double-blind, placebo-controlled study in healthy overweight human subjects

“…3T3-L1 preadipocytes were differentiated to mature adipocytes as described previously [8]. For treatments, the dry powdered Meratrim was dissolved in DMSO and the final concentration of DMSO in the culture was 0.2 % (v/v) in all experiments.…”

Efficacy of an herbal formulation LI10903F containing Dolichos biflorus and Piper betle extracts on weight management

“…The control cultures received only 0.1% (v/v) DMSO as the vehicle. The intracellular lipid accumulation was measured by staining the cells with Oil Red O stain following the method described earlier [10]. …”

“…Equal number of mature adipocytes was treated with different concentrations of either P. Betle or D. biflorus extracts or their formulations in phenol red-free DMEM supplemented with 2% bovine serum albumin (BSA) for 4h. Released glycerol in the cell free culture supernatants was measured using the Adipolysis Assay Kit (Millipore, Billerica, MA) as described previously [10]. …”

Efficacy of a novel herbal formulation for weight loss demonstrated in a 16‐week randomized, double‐blind, placebo‐controlled clinical trial with healthy overweight adults

“…adipogenesis) and potentiate fat breakdown within cells (i.e. lipolysis) in 3T3‐L1 mouse adipocytes . The three extracts shown to be most effective in this screening were combined in various ratios, with the most effective synergistic combination being LI85008F.…”

“…The resulting dietary ingredient inhibits lipogenesis in adipocytes while concurrently antagonizing PPARγ and other lipogenic factors. In addition, it potentiates triglyceride mobilization from fat cells, that is, enhances lipolysis …”