An herbal formula attenuates collagen-induced arthritis via inhibition of JAK2-STAT3 signaling and regulation of Th17 cells in mice

Wu, Xing-Zheng; Shou, Qiyang; Chen, Cuiwei; Cai, Hao; Zhang, Jida; Tang, Suo-Qin; Cai, Baochang; Tang, Dongdong; Cao, Gang

doi:10.18632/oncotarget.17797

Cited by 24 publications

(16 citation statements)

References 15 publications

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“…It is reported that the active ingredients of SD extract include chromones, polyacetylenes, coumarins, and polysaccharides, depending upon different methods of extraction. 16 ,– 30 – 39 Dong et al 22 first reported the structural profile of SDPs and their antagonistic effects against the immunosuppression by the cultural supernatants of melanoma cells on RAW264.7 macrophages.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharides Derived from Saposhnikovia divaricata May Suppress Breast Cancer Through Activating Macrophages

Ding¹,

Guo²,

Jiang³

et al. 2020

OTT

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Section: Discussionmentioning

confidence: 99%

Polysaccharides Derived from Saposhnikovia divaricata May Suppress Breast Cancer Through Activating Macrophages

Ding¹,

Guo²,

Jiang³

et al. 2020

OTT

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“…C0105; Shanghai Beyotime Biological Science & Technology Co., Ltd.), in accordance with the manufacturer's protocol, and subsequently dehydrated with gradient ethanol for 10 sec per concentration gradient (70, 80, 90 and 100%). Sections were then mounted and histopathological changes were observed under a light microscope (magnification, ×100 and ×400) ( 21 ).…”

Section: Methodsmentioning

confidence: 99%

Salvianolic acid B reverses multidrug resistance in nude mice bearing human colon cancer stem cells

et al. 2018

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Salvianolic acid B (SalB) is a water-soluble phenolic compound, extractable from Salvia miltiorrhiza, and has previously been demonstrated to reverse tumor multidrug resistance (MDR) in colon cancer cells. Cancer stem cells (CSCs) are closely associated with drug resistance. Therefore, establishing a nude mouse model bearing human colon CSCs is important for the study of the mechanisms underlying colon cancer drug resistance as well as the reversal of drug resistance. The present study aimed to establish a nude mouse model bearing human colon CSCs and to investigate the effects of SalB on the drug resistance exhibited by the nude mouse model as well as determine its underlying mechanism. Cells from two colon cancer cell lines (LoVo and HCT-116) were cultured in serum-free medium to obtain CSCs-enriched spheroid cells. Following this, nude mice were transplanted with LoVo and HCT-116 colon CSCs to establish the CSC nude mouse model, which was subsequently demonstrated to exhibit MDR. The results of the present study revealed that following treatment with SalB, the chemotherapeutic drug resistance of xenografts was reversed to a certain extent. Western blot analysis was performed to investigate the expression levels of cluster of differentiation (CD)44, CD133, transcription factor sox-2 (SOX2) and ATP-binding cassette sub-family G member 2 (ABCG2) proteins, and the results demonstrated that treatment with SalB downregulated the expression of CD44, SOX2 and ABCG2 proteins in both LoVo and HCT-116 colon CSCs xenografts. In conclusion, the results of the present study revealed that a serum-free suspension method can be performed to successfully isolate colon CSCs. In addition, a nude mice bearing colon CSCs animal model was successfully established, and associated tumors were confirmed to exhibit MDR. Furthermore, SalB was demonstrated to successfully reverse MDR in nude mice bearing LoVo and HCT-116 colon CSCs, as well as suppress the expression of CD44, SOX2 and ABCG2 proteins.

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“…Half of the tissues were fixed in 4% paraformaldehyde, embedded in paraffin, and then sliced up (4 μm). Following, the slices were stained with hematoxylin and eosin, and examined by light microscopy ( Wu et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Evodiamine, a Novel NOTCH3 Methylation Stimulator, Significantly Suppresses Lung Carcinogenesis in Vitro and in Vivo

Xia

Deng

et al. 2018

Front. Pharmacol.

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Lung cancer is a leading cause of cancer-related deaths worldwide. NOTCH3 signaling is mainly expressed in non-small cell lung carcinoma (NSCLC), and has been proposed as a therapeutic target of NSCLC. While, few agents for preventing or treating NSCLC via targeting NOTCH3 signaling are used in modern clinical practice. Evodiamine (EVO), an alkaloid derived from Euodiae Fructus, possesses low toxicity and has long been shown to exert anti-lung cancer activity. However, the underlying anti-lung cancer mechanisms of EVO are not yet fully understood. In this study, we explored the involvement of NOTCH3 signaling in the anti-lung cancer effects of EVO. Urethane-induced lung cancer mouse model and two NSCLC cell models, A549 and H1299, were used to evaluate the in vivo and in vitro anti-lung cancer action of EVO. A DNA methyltransferase inhibitor was employed to investigate the role of NOTCH3 signaling in the anti-lung cancer effects of EVO. Results showed that EVO potently reduced tumor size and tumor numbers in mice, and inhibited NOTCH3 in the tumors. EVO also dramatically reduced cell viability, induced G2/M cell cycle arrest, inhibited cell migration and reduced stemness in cultured NSCLC cells. Mechanistic studies showed that EVO potently inhibited NOTCH3 signaling by activation of DNMTs-induced NOTCH3 methylation. Importantly, inhibition of NOTCH3 methylation in NSCLC cells diminished EVO’s anti-NSCLC effects. Collectively, EVO, a novel NOTCH3 methylation stimulator, exerted potent anti-lung cancer effects partially by inhibiting NOTCH3 signaling. These findings provide new insight into the EVO’s anti-NSCLC action, and suggest a potential role of EVO in lung cancer prevention and treatment.

show abstract

An herbal formula attenuates collagen-induced arthritis via inhibition of JAK2-STAT3 signaling and regulation of Th17 cells in mice

Cited by 24 publications

References 15 publications

Polysaccharides Derived from Saposhnikovia divaricata May Suppress Breast Cancer Through Activating Macrophages

Polysaccharides Derived from Saposhnikovia divaricata May Suppress Breast Cancer Through Activating Macrophages

Salvianolic acid B reverses multidrug resistance in nude mice bearing human colon cancer stem cells

Evodiamine, a Novel NOTCH3 Methylation Stimulator, Significantly Suppresses Lung Carcinogenesis in Vitro and in Vivo

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