An HDAC2-TET1 switch at distinct chromatin regions significantly promotes the maturation of pre-iPS to iPS cells

Tao, Wei; Chen, W.; Wang, X.; Zhang, M.; Chen, J.; Zhu, Shifeng; Chen, L.; Yang, Dandan; Wang, G.; Jia, Wenwen; Yu, Yangyang; Duan, Tao; Wu, Minjuan; Liu, H.; Gao, Shaorong; Kang, Jinsuk

doi:10.1093/nar/gkv430

Cited by 22 publications

(22 citation statements)

References 44 publications

(62 reference statements)

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“…Interestingly, our findings regarding the role of HDAC2 in pre-iPSC reprogramming are in contrast to a recent study (Wei et al, 2015), which identified HDAC2 as a barrier to the reprogramming process. Despite these seemingly contradictory results, however, there are several key differences between our study and theirs that should help explain these opposite findings.…”

Section: Discussioncontrasting

confidence: 99%

The SIN3A/HDAC Corepressor Complex Functionally Cooperates with NANOG to Promote Pluripotency

et al. 2017

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SUMMARY Although Sin3a is required for survival of early embryos and embryonic stem cells (ESCs), the role of Sin3a in the maintenance and establishment of pluripotency remains unclear. Here we find that the Sin3a/HDAC corepressor complex maintains ESC pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the Sin3a/HDAC corepressor complex are enriched in an extended Nanog interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for Sin3a and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, Nanog and Sin3a co-occupy transcriptionally active pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting pluripotency.

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Section: Discussioncontrasting

confidence: 99%

The SIN3A/HDAC Corepressor Complex Functionally Cooperates with NANOG to Promote Pluripotency

et al. 2017

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“…Further, we used Co-IP analysis to demonstrate that TET1/PCNA complex is effective in the modulation of proliferation as a highly specific manner. Histone acetylation dynamic played important epigenetic regulatory roles in induced pluripotent stem cells (iPSCs) formation, previous study showed HDAC2 was antagonism with TET1 in the same gene regions, so TET1 promote iPSCs formation by decreasing the HDAC2 binding sites, and change histone acetylation of key genes60. In our study, Hdac1 was recruited specifically by mTET1 and formed protein complex to regulate histone acetylation, moreover, the complex bind to key genes and regulate gene and protein expression of mGSCs.…”

Section: Discussionmentioning

confidence: 57%

The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation

Zheng

Zhai

et al. 2016

Sci Rep

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Epigenetic modification plays key roles in spermatogenesis, especially DNA methylation dynamic is important in sustaining normal spermatogenesis. Ten-eleven translocation 1 (Tet1) is not only a key demethylase, which works in specific gene regions, but also crosstalks with partners to regulate epigenetic progress as protein complexes. Dairy goat is an important livestock in China, while the unstable culture system in vitro inhibits optimization of new dairy goat species. The study of epigenetic modification in male germline stem cells (mGSCs) is beneficial to the optimization of adult stem cell culture system in vitro, and the improvement of sperm quality and breeding of selected livestock. In our study, we not only analyzed the morphology, gene expression, DNA methylation and histone methylation dynamic in mouse Tet1 (mTet1) modified mGSCs, we also analyzed the stemness ability by in vivo transplantation and explored the functional mechanism of Tet1 in dairy goat mGSCs. The results showed mTet1 modified mGSCs had better self-renewal and proliferation ability than wild-type mGSCs, mTet1 could also up-regulate JMJD3 to decrease H3K27me3, which also showed to suppress the MEK-ERK pathway. Furthermore, Co-IP analysis demonstrated that TET1 interact with PCNA and HDAC1 by forming protein complexes to comprehensively regulate dairy goat mGSCs and spermatogenesis.

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“…Currently, there are three main types of stem cells employed for 3D culture, including ESCs obtained from embryos, adult stem cells derived from specific tissues like bone marrow, heart, brain, adipose tissue, kidney, lung, liver, gastrointestinal tract, and pancreas, and iPSCs derived from normal karyocytes . Pluripotent stem cells with the ability to self‐renew and to differentiate into multiple different cell lineages have been identified in most adult human tissues and organs .…”

Section: Stem Cell Biology In 3d Culturementioning

confidence: 99%

Looking into the Future: Toward Advanced 3D Biomaterials for Stem‐Cell‐Based Regenerative Medicine

et al. 2018

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Stem-cell-based therapies have the potential to provide novel solutions for the treatment of a variety of diseases, but the main obstacles to such therapies lie in the uncontrolled differentiation and functional engraftment of implanted tissues. The physicochemical microenvironment controls the self-renewal and differentiation of stem cells, and the key step in mimicking the stem cell microenvironment is to construct a more physiologically relevant 3D culture system. Material-based 3D assemblies of stem cells facilitate the cellular interactions that promote morphogenesis and tissue organization in a similar manner to that which occurs during embryogenesis. Both natural and artificial materials can be used to create 3D scaffolds, and synthetic organic and inorganic porous materials are the two main kinds of artificial materials. Nanotechnology provides new opportunities to design novel advanced materials with special physicochemical properties for 3D stem cell culture and transplantation. Herein, the advances and advantages of 3D scaffold materials, especially with respect to stem-cell-based therapies, are first outlined. Second, the stem cell biology in 3D scaffold materials is reviewed. Third, the progress and basic principles of developing 3D scaffold materials for clinical applications in tissue engineering and regenerative medicine are reviewed.

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An HDAC2-TET1 switch at distinct chromatin regions significantly promotes the maturation of pre-iPS to iPS cells

Cited by 22 publications

References 44 publications

The SIN3A/HDAC Corepressor Complex Functionally Cooperates with NANOG to Promote Pluripotency

The SIN3A/HDAC Corepressor Complex Functionally Cooperates with NANOG to Promote Pluripotency

The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation

Looking into the Future: Toward Advanced 3D Biomaterials for Stem‐Cell‐Based Regenerative Medicine

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