An fluorescent aptasensor for sensitive detection of tumor marker based on the FRET of a sandwich structured QDs-AFP-AuNPs

Zhou, Lu; Ji, Feihong; Zhang, Tao; Wang, Feng; Li, Yuchuan; Yu, Zhixin; Jin, Xiaoping; Ruan, Bing

doi:10.1016/j.talanta.2019.01.012

Cited by 67 publications

(23 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another attempt to detect AFP, Zhou et al combined both antibody–antigen and protein–DNA interactions in a FRET sensing system, where the aptamer labeled luminescent CdTe quantum dots (QDs) were the donors and AFP antibody grafted AuNPs were the acceptors. 246 In the presence of AFP antigen, its affinity to antibody and aptamer reduced the distance between the two kinds of NPs. As a result, AuNPs quenched the fluorescence of QDs.…”

Section: Aunps-based Optical Nanosensors For Food and Health Safety M...mentioning

confidence: 99%

See 1 more Smart Citation

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Aunps-based Optical Nanosensors For Food and Health Safety M...mentioning

confidence: 99%

“…Moreover, it also showed a satisfying performance in human serum samples. 246 It is worth noting that in this study, the author used 2 sites of recognition instead of one, which increased the specificity of the sensing method.…”

Section: Aunps-based Optical Nanosensors For Food and Health Safety M...mentioning

confidence: 99%

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Their presence or quantitative changes may indicate the nature of the tumor, thereby contributing to tumor diagnosis, prognosis judgment, and treatment 8 , 9 . There were many reports about the detection of tumor markers, such as carcinoembryonic antigen (CEA) 10 , alpha fetoprotein (AFP) 11 , cancer antigen (CA) 125 12 and so on. Among many molecular markers, CEA is one of the tumor markers for many cancers, which can be used to indicate postoperative tumor recurrence 13 .…”

Section: Introductionmentioning

confidence: 99%

Gold nanorods-mediated efficient synergistic immunotherapy for detection and inhibition of postoperative tumor recurrence

Zhang

Wang

Tian

et al. 2021

Acta Pharmaceutica Sinica B

View full text Add to dashboard Cite

Tumor recurrence after surgery is the main cause of treatment failure. However, the initial stage of recurrence is not easy to detect, and it is difficult to cure in the late stage. In order to improve the life quality of postoperative patients, an efficient synergistic immunotherapy was developed to achieve early diagnosis and treatment of post-surgical tumor recurrence, simultaneously. In this paper, two kinds of theranostic agents based on gold nanorods (AuNRs) platform were prepared. AuNRs and quantum dots (QDs) in one agent was used for the detection of carcinoembryonic antigen (CEA), using fluorescence resonance energy transfer (FRET) technology to indicate the occurrence of in situ recurrence, while AuNRs in the other agent was used for photothermal therapy (PTT), together with anti-PDL1 mediated immunotherapy to alleviate the process of tumor metastasis. A series of assays indicated that this synergistic immunotherapy could induce tumor cell death and the increased generation of CD3 + /CD4 + T-lymphocytes and CD3 + /CD8 + T-lymphocytes. Besides, more immune factors (IL-2, IL-6, and IFN- γ ) produced by synergistic immunotherapy were secreted than mono-immunotherapy. This cooperative immunotherapy strategy could be utilized for diagnosis and treatment of postoperative tumor recurrence at the same time, providing a new perspective for basic and clinical research.

show abstract

“…To date, many fluorescent materials have been employed to develop various fluorescent biosensors, including organic dyes [13,14], up-converting nanoparticles [15,16] and fluorescent microspheres [17,18], etc. As is well known, quantum dots (QDs) are an excellent fluorescent probe with the advantages of wide excitation range, strong fluorescent signal and long fluorescent lifetime, and are often used for fluorescent biosensing [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Biosensor Based on Magnetic Nanoparticle Separation, Quantum Dots Labeling and MnO2 Nanoflower Amplification for Rapid and Sensitive Detection of Salmonella Typhimurium

Huang

et al. 2020

Micromachines

View full text Add to dashboard Cite

Screening of foodborne pathogens is an effective way to prevent microbial food poisoning. A microfluidic biosensor was developed for rapid and sensitive detection of Salmonella Typhimurium using quantum dots (QDs) as fluorescent probes for sensor readout and manganese dioxide nanoflowers (MnO2 NFs) and as QDs nanocarriers for signal amplification. Prior to testing, amino-modified MnO2 nanoflowers (MnO2-NH2 NFs) were conjugated with carboxyl-modified QDs through EDC/NHSS method to form MnO2-QD NFs, and MnO2-QD NFs were functionalized with polyclonal antibodies (pAbs) to form MnO2-QD-pAb NFs. First, the mixture of target Salmonella Typhimurium cells and magnetic nanoparticles (MNPs) modified with monoclonal antibodies (mAbs) was injected with MnO2-QD-pAb NFs into a microfluidic chip to form MNP-bacteria-QD-MnO2 complexes. Then, glutathione (GSH) was injected to dissolve MnO2 on the complexes into Mn2+, resulting in the release of QDs. Finally, fluorescent intensity of the released QDs was measured using the fluorescent detector to determine the amount of Salmonella. A linear relationship between fluorescent intensity and bacterial concentration from 1.0 × 102 to 1.0 × 107 CFU/mL was found with a low detection limit of 43 CFU/mL and mean recovery of 99.7% for Salmonella in spiked chicken meats, indicating the feasibility of this biosensor for practical applications.

show abstract

An fluorescent aptasensor for sensitive detection of tumor marker based on the FRET of a sandwich structured QDs-AFP-AuNPs

Cited by 67 publications

References 40 publications

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

Gold nanorods-mediated efficient synergistic immunotherapy for detection and inhibition of postoperative tumor recurrence

A Microfluidic Biosensor Based on Magnetic Nanoparticle Separation, Quantum Dots Labeling and MnO2 Nanoflower Amplification for Rapid and Sensitive Detection of Salmonella Typhimurium

Contact Info

Product

Resources

About