An eye color gene for the detection of transgenic non-drosophilid insects

White, Lisa D.; Coates, Craig J.; Atkinson, Peter W.; O’Brochta, David A.

doi:10.1016/s0965-1748(96)00039-2

Cited by 29 publications

(14 citation statements)

References 19 publications

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“…Since the early days of transposon-mediated germline transformation in D. melanogaster, eye-color genes have been the gold standard for transformation markers (RUBIN AND SPRADLING 1982;WHITE et al 1996;BESANSKY et al 1997;SUMITANI et al 2005). Indeed, even with the advent of fluorescent markers, eye-specific expression has been favored over other promoters, via the development of the 3xP3 artificial eye-specific promoter, since it is typically easy to identify (BERGHAMMER et al 1999;HORN et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Window to the Fluorescence: TheWhiteEye-Color Gene of Western Corn Rootworm,Diabrotica virgifera virgifera

Grubbs

Chu

Lorenzen

2019

Preprint

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Eye-color mutations have proven useful in multiple insect species to help facilitate the development and use of transgenic tools for functional genomics. While there is species-specific variation in the pigments used to color insect eyes, every species studied thus far requires an ortholog of the ABC transporter gene white for proper pigmentation of the eyes. Previously, we generated transgenic western corn rootworm, Diabrotica virgifera virgifera, and found that their wild-type eye color obscured our ability to visualize a fluorescent marker driven by the widely used 3xP3 eye-specific promoter. Therefore, we sought to identify the D. v. virgifera ortholog of white (Dvvw). Here we report the discovery, cloning, and analysis of Dvvw cDNA and promoter.We also utilize RNA interference to knock down Dvvw mRNA in a transgenic strain, thereby demonstrating the utility of eye-color mutations when developing transgenic technologies.

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Section: Introductionmentioning

confidence: 99%

Window to the Fluorescence: TheWhiteEye-Color Gene of Western Corn Rootworm,Diabrotica virgifera virgifera

Grubbs

Chu

Lorenzen

2019

Preprint

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“…A number of genetic loci initially found to influence Drosophila eye coloration have since been shown to correspond to genes encoding biosynthetic pathway enzymes, ATP‐binding cassette (ABC) transporters, and vesicular transport proteins (Lloyd et al, ). Given the ease of visual screening, eye color mutations have been used as genetic markers for germ‐line transformation (Cornel, Benedict, Rafferty, Howells, & Collins, ; Sethuraman & O'Brochta, ; White, Coates, Atkinson, & O'Brochta, ; Zwiebel et al, ), the development and assessment of gene knockdown/silencing methods (Adrianos, Lorenzen, & Oppert, ; Colinet et al, ; Dong & Friedrich, ; Fabrick, Kanost, & Baker, ; Khan, Reichelt, & Heckel, ; Perera, Little, & Pierce, ), and fertility and fecundity studies (Khanh, Bressac, & Chevrier, ; Pires, Abrão, Machado, Schofield, & Diotaiuti, ), and have been proposed as markers for field and dispersal monitoring (Shimizu & Kawasaki, ; Snodgrass, ), and as experimental models for aging (Campesan et al, ; Navrotskaya & Oxenkrug, ; Oxenkrug, ; Savvateeva et al, ).…”

Section: Introductionmentioning

confidence: 99%

RNA interference‐mediated knockdown of eye coloration genes in the western tarnished plant bug (Lygus hesperus Knight)

Brent

Hull

2018

Arch Insect Biochem Physiol

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Insect eye coloration arises from the accumulation of various pigments. A number of genes that function in the biosynthesis (vermilion, cinnabar, and cardinal) and importation (karmoisin, white, scarlet, and brown) of these pigments, and their precursors, have been identified in diverse species and used as markers for transgenesis and gene editing. To examine their suitability as visible markers in Lygus hesperus Knight (western tarnished plant bug), transcriptomic data were screened for sequences exhibiting homology with the Drosophila melanogaster proteins. Complete open reading frames encoding putative homologs for all seven genes were identified. Bioinformaticbased sequence and phylogenetic analyses supported initial annotations as eye coloration genes. Consistent with their proposed role, each of the genes was expressed in adult heads as well as throughout nymphal and adult development. Adult eyes of those injected with double-stranded RNAs (dsRNAs) for karmoisin, vermilion, cinnabar, cardinal, and scarlet were characterized by a red band along the medial margin extending from the rostral terminus to the antenna. In contrast, eyes of insects injected with dsRNAs for both white and brown were a uniform light brown. White knockdown also produced cuticular and behavioral defects. Based on its expression profile and robust visible phenotype, cardinal would likely prove to be the most suitable marker for developing gene editing methods in Lygus species.

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“…Tryptophan oxygenase is an essential amino acid of insects as its absence or deficiency results in eye color defects in Drosophila and other insect species (Summers et al 1982; Miyashita et al 1994; White et al 1996; Lorenzen et al 2002; Fabrick et al 2004). In the present study, the Bombyx mori tryptophan oxygenases homolog ( Bmto ) was successfully cloned and analyzed.…”

Section: Discussionmentioning

confidence: 99%

“…Loukeris et al (1995) developed a transformation system for the medfly Ceratitis capitata with the eye color gene white. White et al (1996) linked vermilion cDNA of D. melanogaster to the inducible hsp82 promoter of D. pseudoobsura . This marker rescued adult eye color in a strain of Musca domestica that was homozygous for a mutant tryptophane oxygenase gene (White et al 1996).…”

Section: Discussionmentioning

confidence: 99%

“…White et al (1996) linked vermilion cDNA of D. melanogaster to the inducible hsp82 promoter of D. pseudoobsura . This marker rescued adult eye color in a strain of Musca domestica that was homozygous for a mutant tryptophane oxygenase gene (White et al 1996). Besansky et al (1997) placed Anopheles gambiae cDNA encoding tryptophan oxygenase under the control of the constitutive baculovirus promoter, ie-1 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Cloning and Analysis of theTryptophan oxygenaseGene in the Silkworm,Bombyx mori

Liu

Meng

Niu

et al. 2008

Journal of Insect Science

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A Bombyx mori L. (Lepidoptera: Bombycidae) gene encoding tryptophan oxygenase has been molecularly cloned and analyzed. The tryptophan oxygenase cDNA had 1374 nucleotides that encoded a 401 amino acid protein with an estimated molecular mass of 46.47 kDa and a PI of 5.88. RT-PCR analysis showed that the B. mori tryptophan oxygenase gene was transcribed in all examined stages. Tryptophan oxygenase proteins are relatively well conserved among different orders of arthropods.

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An eye color gene for the detection of transgenic non-drosophilid insects

Cited by 29 publications

References 19 publications

Window to the Fluorescence: TheWhiteEye-Color Gene of Western Corn Rootworm,Diabrotica virgifera virgifera

Window to the Fluorescence: TheWhiteEye-Color Gene of Western Corn Rootworm,Diabrotica virgifera virgifera

RNA interference‐mediated knockdown of eye coloration genes in the western tarnished plant bug (Lygus hesperus Knight)

Molecular Cloning and Analysis of theTryptophan oxygenaseGene in the Silkworm,Bombyx mori

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