An Extractive Spectrophotometric Method for the Determination of Pymetrozine in Various Ecological Samples of Bilaspur District (C.G.)

Abstract

Pesticides are designated mixture of substances for extenuating and destroying any group of pests such as insects and vegetation. Pymetrozine [6-methyl-4-[(E)-pyridin-3-ylmethylideneamino]-2, 5-dihydro-1, 2, 4-triazin-3-one] is a pyridine azomethine compound which represents a class of insecticide. A Spectrophotometric method has been developed on the modified Fujiwara reaction for the determination of pymetrozine. Pymetrozine directly reacts with chloroform the 45-500C turns out the violet color in the formation of Schiff’s Base (Glutaconic Aldehyde). In the present reaction, the violet color obtained is discharged with a few drops of acetic acid followed by the addition of p- nitro aniline reagent which gives yellow color dyes. The absorption maxima of yellow-colored dye measured at 430 nm. Beer’s law is obeyed over the range of 1 to 10 µg per 10 ml of pymetrozine. The molar absorptivity of the colored system is 1.38×105 L mol-1 and Sandall’s sensitivity is 1.0×10-3µg cm-2. This method is free from the interference of other interfering and can be successfully applied for the determination of various ecological samples.