An extracellular yellow laccase with potent dye decolorizing ability from the fungus Leucoagaricus naucinus LAC-04

Ning, Ying-Jie; Wang, Shanshan; Chen, Qingjun; Ling, Zhuoren; Wang, Shou-Nan; Wang, Wenping; Zhang, Guoqing; Zhu, Mengjuan

doi:10.1016/j.ijbiomac.2016.09.046

Cited by 21 publications

(14 citation statements)

References 42 publications

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“…Among Leucoagaricus naucinus (Fr.) Singer is a mycorrhizal fungus also known as Leucoagaricus leucothites and abundant in the conifer forests [15,16]. Previous studies suggest that L. leucothites have highest free radical scavenging ability and anti-oxidant activity and growing on the metal-rich soils [17,18].…”

Section: Introductionmentioning

confidence: 99%

Sexual Differences in Growth and Physiological Characteristics of Populus cathayana under Pb Stress and Leucoagaricus sp. Colonization

Feng¹,

Dou²,

Chi³

et al. 2021

Pol. J. Environ. Stud.

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Populus cathayana was used as a model species to investigate sexual differences in plant growth and physiological responses to lead (Pb) stress (0, 150 and 300 mg Pb 2+ kg-1 dry soil) with mycorrhizal (Myc) fungi colonization by Leucoagaricus sp. Results showed that high concentration of Pb stress (300 mg/ kg) caused disorder in photosynthesis, inhibited plant biomass, changed antioxidant enzyme activities and increased Malondialdehyde (MDA) content in both sexes, particularly in females. Male saplings showed greater biomass, gas exchange capacity, antioxidant enzyme activity and lower MDA content when exposed to Pb stress alone and to a combination of Pb and Myc fungi treatment. Furthermore, Leucoagaricus sp. inoculation alleviated Pb toxicity in both sexes by increasing biomass accumulation, promoting gas exchange capacity, enhancing antioxidant enzyme activities and decreasing relative electrolyte leakage under low concentration (150 mg/kg) and high concentration Pb stress. Inoculation with Leucoagaricus sp. promoted Pb uptake in both sexes, and induced the sequestration of more toxic Pb in the root systems in males. Therefore, P. cathayana males associated with Leucoagaricus sp. performed best under high concentration of Pb stress and more suitably restore Pb-polluted soils than inoculated females due to the higher Pb uptake capacity and greater growth traits.

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Section: Introductionmentioning

confidence: 99%

Sexual Differences in Growth and Physiological Characteristics of Populus cathayana under Pb Stress and Leucoagaricus sp. Colonization

Feng¹,

Dou²,

Chi³

et al. 2021

Pol. J. Environ. Stud.

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“…Para o ensaio de lacase, 50 µL do filtrado foi incubado com 100 µL de solução 1 mM de ácido 2,2'-azino-bis(3etilbenzotiazolina-6-sulfônico) (ABTS) por 10 minutos, de acordo com Ning et al (2016). A reação foi interrompida pela adição de 150 µL de ácido tricloroacético (TCA) 10% a leitura da amostra foi realizada em leitor de placas (ScpectraMax, Molecular Devices) em 405 nm.…”

Section: Atividade De Lacaseunclassified

“…A unidade de atividade enzimática (U) foi definida como a quantidade de enzima necessária para aumentar a absorbância em 1 unidade sob as condições do ensaio (NING et al, 2016).…”

Section: Atividade De Lacaseunclassified

Transcriptoma e expressão recombinante de peptidases do fungo Phanerochaete chrysosporium em Pichia pastoris

Simões¹

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metanol. A atividade enzimática dos clones contendo a construção pPICZαA-DN10508 (aspartil peptidase) mostraram que os clones 1 (16,00 ± 3,27 U/mL) ,2 (14,44 ± 1,12 U/mL), 11 (14,27± 0,315 U/mL), 4 (14,27 ± 0,086 U/mL) e 5 (14,15 ± 2,15 U/mL) obtiveram a maior atividade enzimática. A atividade enzimática dos clones contendo pPICZαA-DN7040 (cisteíno peptidase) não apresentaram resultados promissores. Nas condições avaliadas foi possível induzir a expressão de diferentes classes enzimáticas. A biblioteca de transcritos gerou mais de 33 mil sequências e através dela, foi possível selecionar e expressar duas peptidases (aspartil e cisteíno peptidases) para a expressão heteróloga. Palavras chave: Peptidases recombinantes, Aspartil peptidase, Cisteíno peptidase iii ABSTRACT SIMÕES, F.A.O. Trancriptome and recombinant peptidase expression from the fungus Phanerochaete chrysosporium in Pichia pastoris. 2019. 87p. Dissertation (Master). Faculdade de Ciências Farmacêuticas de Ribeirão Preto -Universidade de São Paulo, Ribeirão Preto, 2019.Fungi can be found in a variety of habitats and have great genetic diversity. These microorganisms produce a variety of enzymes and some of these may work under specific temperature and pH conditions. Phanerochaete chrysosporium can completely degrade lignin. Its genome possess a large repertoire of genes related to lignin metabolism including cytochrome p450, oxidoreductose and hydrolases, which includes peptidase enzymes. Peptidase enzymes are involved in various biological pathways and have industrial applications (textile and food industry). Our research group have described two native peptidases (aspartyl and cysteine peptidase) with great potential for industrial application. Recombinant protein production could provide an efficient way for enzyme production and purification. Pichia patoris expression system is widely used for recombinant protein expression, and it is described as efficient for both production and secretion of recombinant enzymes, it also is able to execute post-translational modifications. The aim of this study was to perform transcriptome analyses of the fungus Phanerochaete chrysosporium to identify and express two heterologous peptidase (aspartic and cysteine peptidase) in P. pastoris for biochemical and functional studies. P. chrysosporium was submitted to different bioprocesses supplemented with feather meal (1%), casein (1%), avicel (1%), or olive oil (1%). Enzymatic activities of peptidases, laccases, amylases, lipases, CMCases, xylanases and pectinases were determined. All activities were detected, except lipase and pectinase activities. Extracts supplemented with feather meal were purified by ion exchange chromatography and the N-terminal sequence of aspartic and cysteine peptidases were determined by Edman degradation. The mycelium was used for RNA extraction and cDNA synthesis. A transcript library was built using cDNA sequences, which generated 33840 sequences. The library was analyzed by UNIPROT and 7327 sequences were identified (52% non-enzymes and 4...

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“…Trametes pubescens LAP2 é 9 vezes maior (Galhaup et al, 2002). Apesar da maioria das lacases fúngicas serem funcionais em valores de pH mais ácido entre 2-5 (Ning et al, 2016;Sadhasivam et al, 2008). Yadav et al (2019) relataram a atividade máxima de lacase em pH 6,0, enquanto os fungos Botrytis cinerea 61-34.…”

Section: Cinética Enzimáticaunclassified

“…Leucoagaricus naucinus LAC-04 com temperatura ótima a 30°C, seguido por 40°C e 50°C respectivamente. (Ning et al, 2016).…”

Section: Cinética Enzimáticaunclassified

Produção, purificação e caracterização da enzima lacase do fungo >i<Cochliobolus sativus>/i<

Sampaio¹

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An extracellular yellow laccase with potent dye decolorizing ability from the fungus Leucoagaricus naucinus LAC-04

Cited by 21 publications

References 42 publications

Sexual Differences in Growth and Physiological Characteristics of <i>Populus cathayana</i> under Pb Stress and <i>Leucoagaricus</i> sp. Colonization

Sexual Differences in Growth and Physiological Characteristics of <i>Populus cathayana</i> under Pb Stress and <i>Leucoagaricus</i> sp. Colonization

Transcriptoma e expressão recombinante de peptidases do fungo Phanerochaete chrysosporium em Pichia pastoris

Produção, purificação e caracterização da enzima lacase do fungo >i<Cochliobolus sativus>/i<

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