An extracellular oxygen carrier during prolonged pulmonary preservation improves post-transplant lung function

Watanabe, Y.; Galasso, M.; Watanabe, Tatsuaki; Chen, Manyin; Fan, Eddy; Brochard, Laurent; Ramadan, Khaled; Ribeiro, Rafaela Vanin Pinto; Stansfield, William E.; Gokhale, H.; Gazzalle, Anajara; Waddell, Thomas K.; Liu, Mingyao; Keshavjee, S.; Cypel, Marcelo

doi:10.1016/j.healun.2020.03.027

Cited by 18 publications

(11 citation statements)

References 28 publications

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“…The use of HEMO 2 Life® is also interesting due to its anti-oxidative effect preventing cytokine storm induced by the SARS-CoV-2. Indeed, HEMO 2 Life® has a superoxide-dismutase activity that can address this problem and a recent Canadian study revealed an anti-IL6 action during lung transplantation [11] .…”

Section: The Hypothesismentioning

confidence: 99%

Combating hypoxemia in COVID-19 patients with a natural oxygen carrier, HEMO2Life® (M101)

Lupon

Lellouch

Zal³

et al. 2021

Medical Hypotheses

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Section: The Hypothesismentioning

confidence: 99%

Combating hypoxemia in COVID-19 patients with a natural oxygen carrier, HEMO2Life® (M101)

Lupon

Lellouch

Zal³

et al. 2021

Medical Hypotheses

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“…In the context of inflammation control, supplementation with M101 has shown reduced inflammation and fibrosis in kidney epithelium during static preservation 34 . Moreover, lung grafts preservation and perfusion with M101 decreased edema formation, apoptotic cell death and plasma levels of pro-inflammatory IL-6, hence, improving post-transplant lung function 37 . Furthermore, no immunogenic, allergenic or prothrombotic effects have been reported with in vivo administration 31 or in the recently carried out first human trial of M101 in kidney transplantation 38 .…”

Section: Introductionmentioning

confidence: 95%

A therapeutic oxygen carrier isolated from Arenicola marina decreased P. gingivalis induced inflammation and tissue destruction

Batool

Stutz

Petit

et al. 2020

Sci Rep

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The control of inflammation and infection is crucial for periodontal wound healing and regeneration. M101, an oxygen carrier derived from Arenicola marina, was tested for its anti-inflammatory and anti-infectious potential based on its anti-oxidative and tissue oxygenation properties. In vitro, no cytotoxicity was observed in oral epithelial cells (EC) treated with M101. M101 (1 g/L) reduced significantly the gene expression of pro-inflammatory markers such as TNF-α, NF-κΒ and RANKL in P. gingivalis-LPS stimulated and P. gingivalis-infected EC. The proteome array revealed significant down-regulation of pro-inflammatory cytokines (IL-1β and IL-8) and chemokine ligands (RANTES and IP-10), and upregulation of pro-healing mediators (PDGF-BB, TGF-β1, IL-10, IL-2, IL-4, IL-11 and IL-15) and, extracellular and immune modulators (TIMP-2, M-CSF and ICAM-1). M101 significantly increased the gene expression of Resolvin-E1 receptor. Furthermore, M101 treatment reduced P. gingivalis biofilm growth over glass surface, observed with live/dead analysis and by decreased P. gingivalis 16 s rRNA expression (51.7%) (p < 0.05). In mice, M101 reduced the clinical abscess size (50.2%) in P. gingivalis-induced calvarial lesion concomitant with a decreased inflammatory score evaluated through histomorphometric analysis, thus, improving soft tissue and bone healing response. Therefore, M101 may be a novel therapeutic agent that could be beneficial in the management of P. gingivalis associated diseases.

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“…MP may be used as a platform for the dynamic circulation of isolated organs during preservation, and it induces a more even distribution of HBOCs in blood vessels, promotes the transport and diffusion of oxygen in the microcirculation, and improves the quality of the graft and the prognosis of the recipient ( Fontes et al, 2015 ). Thus, the application of preservation solutions with HBOCs is similar to that of conventional preservation solutions and can be used directly for static cold preservation and mechanical perfusion of isolated organs without significant changes in protocol or equipment ( Ali et al, 2020 ). HBOCs, as a class of oxygen carriers close to physiological conditions, can be used in various types of solid organs with multiple protocols for preservation.…”

Section: Hbocs In Solid Organ Transplantationmentioning

confidence: 99%

“…Therefore, the protective effect of M101 on IRI prevented the occurrence of PGD. Another study reported that SCS for up to 36 h after adding M101 reduced the risk of PGD after lung transplantation, which may be related to a significant decrease in circulating interleukin-6 (IL-6) levels ( Ali et al, 2020 ). Compared to the standard lung preservation program, the isolated lung was washed and preserved with an M101 preservation solution and evaluated using ex vivo lung perfusion (EVLP) for 12 h; the M101 lung showed better lung function after transplantation.…”

Section: Hbocs In Solid Organ Transplantationmentioning

confidence: 99%

“…Ischemia and hypoxia are the leading causes of graft damage during the storage of isolated organs and may lead to a variety of destructive effects, such as ion imbalance, uncoupling of mitochondria, activation of endothelial cells, activation of various cell death programs, and proinflammatory immune responses ( Kalisvaart et al, 2018 ; Lavallard et al, 2020 ). The current static cold storage (SCS) of grafts reduces enzyme activity and oxygen consumption, but the demand for energy metabolism and oxygen continues while cells maintain ion homeostasis and membrane potential ( Kasil et al, 2019 ; Ali et al, 2020 ). Several methods for supplementing oxygen in organ preservation solutions have been reported.…”

Section: Introductionmentioning

confidence: 99%

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Hemoglobin-Based Oxygen Carriers: Potential Applications in Solid Organ Preservation

Cao

Wang

et al. 2021

Front. Pharmacol.

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Ameliorating graft injury induced by ischemia and hypoxia, expanding the donor pool, and improving graft quality and recipient prognosis are still goals pursued by the transplant community. The preservation of organs during this process from donor to recipient is critical to the prognosis of both the graft and the recipient. At present, static cold storage, which is most widely used in clinical practice, not only reduces cell metabolism and oxygen demand through low temperature but also prevents cell edema and resists apoptosis through the application of traditional preservation solutions, but these do not improve hypoxia and increase oxygenation of the donor organ. In recent years, improving the ischemia and hypoxia of grafts during preservation and repairing the quality of marginal donor organs have been of great concern. Hemoglobin-based oxygen carriers (HBOCs) are “made of” natural hemoglobins that were originally developed as blood substitutes but have been extended to a variety of hypoxic clinical situations due to their ability to release oxygen. Compared with traditional preservation protocols, the addition of HBOCs to traditional preservation protocols provides more oxygen to organs to meet their energy metabolic needs, prolong preservation time, reduce ischemia–reperfusion injury to grafts, improve graft quality, and even increase the number of transplantable donors. The focus of the present study was to review the potential applications of HBOCs in solid organ preservation and provide new approaches to understanding the mechanism of the promising strategies for organ preservation.

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An extracellular oxygen carrier during prolonged pulmonary preservation improves post-transplant lung function

Cited by 18 publications

References 28 publications

Combating hypoxemia in COVID-19 patients with a natural oxygen carrier, HEMO2Life® (M101)

Combating hypoxemia in COVID-19 patients with a natural oxygen carrier, HEMO2Life® (M101)

A therapeutic oxygen carrier isolated from Arenicola marina decreased P. gingivalis induced inflammation and tissue destruction

Hemoglobin-Based Oxygen Carriers: Potential Applications in Solid Organ Preservation

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