An extracellular matrix-inspired self-healing composite hydrogel for enhanced platelet-rich plasma-mediated chronic diabetic wound treatment

Zhang, Ya; Wang, Zili; Deng, Ze‐Peng; Wang, Zilin; Song, Fei; Zhu, Lili

doi:10.1016/j.carbpol.2023.120973

Cited by 19 publications

(13 citation statements)

References 58 publications

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“…Zhiyong et al 54 also reported that controlled and sustained release of PRP would accelerate skin collagen synthesis and deposition at the wound site. In another study, Zhang et al 55 designed an injectable, self-healing, and non-specific tissue-adhesive hydrogel based on chondroitin sulfate/carboxymethyl chitosan-PRP and demonstrated that by PRP delivery and its various growth factor the formation of granulation tissues, collagen deposition and angiogenesis were promoted. Similarly, Xu et al 56 examined the effect of autologous PRP on full-thickness wound healing and showed by adding PRP at wound site, the re-epithelialization, extra cellular matrix formation and synthesis collagen accelerated significantly.…”

Section: Resultsmentioning

confidence: 99%

Enhanced wound regeneration by PGS/PLA fiber dressing containing platelet-rich plasma: an in vitro study

Heydari,

Zargar Kharazi,

Shariati

2024

Sci Rep

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Novel wound dressings with therapeutic effects are being continually designed to improve the wound healing process. In this study, the structural, chemical, physical, and biological properties of an electrospun poly glycerol sebacate/poly lactide acid/platelet-rich plasma (PGS/PLA-PRP) nanofibers were evaluated to determine its impacts on in vitro wound healing. Results revealed desirable cell viability in the Fibroblast (L929) and macrophage (RAW-264.7) cell lines as well as human umbilical vein endothelial cells (HUVEC). Cell migration was evident in the scratch assay (L929 cell line) so that it promoted scratch contraction to accelerate in vitro wound healing. Moreover, addition of PRP to the fiber structure led to enhanced collagen deposition (~ 2 times) in comparison with PGS/PLA scaffolds. While by addition PRP to PGS/PLA fibers not only decreased the expression levels of pro-inflammatory cytokines (IL-6 and TNF-α) in RAW-264.7 cells but also led to significantly increased levels of cytokine (IL-10) and the growth factor (TGF-β), which are related to the anti-inflammatory phase (M2 phenotype). Finally, PGS/PLA-PRP was found to induce a significant level of angiogenesis by forming branching points, loops, and tubes. Based on the results obtained, the PGS/PLA-PRP dressing developed might be a promising evolution in skin tissue engineering ensuring improved wound healing and tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

Enhanced wound regeneration by PGS/PLA fiber dressing containing platelet-rich plasma: an in vitro study

Heydari,

Zargar Kharazi,

Shariati

2024

Sci Rep

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“…Finally, the concentrations of VEGF and TGF-β were measured using an ELISA kit following the manufacturer's protocol. 47–49…”

Section: Methodsmentioning

confidence: 99%

Engineering a platelet-rich plasma-based multifunctional injectable hydrogel with photothermal, antibacterial, and antioxidant properties for skin regeneration

Alinezhad,

Esmaeilzadeh,

Bagheri

et al. 2023

Biomater. Sci.

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“…CC and OCS powders were dissolved in distilled water, separately, to prepare solutions with certain concentrations. CC solutions (15, 30, and 60 mg/mL) were mixed with the OCS solution (30 mg/mL) at a volume ratio of 1:1 to prepare ICH …”

Section: Methodsmentioning

confidence: 99%

“…CC solutions (15, 30, and 60 mg/ mL) were mixed with the OCS solution (30 mg/mL) at a volume ratio of 1:1 to prepare ICH. 21 2.4. Preparation and Characterization of siBAK1@ LPs@ICH.…”

Section: ■ Introductionmentioning

confidence: 99%

Injectable Hydrogel Containing RNA-Loaded Liposomes Alleviates Chondrocyte Inflammation by Disrupting BAK1-Mediated mtDNA Maintenance

Wang,

Chen,

Chen

2023

ACS Appl. Nano Mater.

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Osteoarthritis (OA) causes disability and morbidity. The main therapy for OA is chronic treatment, focusing on articular cartilage protection and inflammation control. Mitochondrial DNA (mtDNA) damage plays an important role in cGAS-STING-induced inflammation. Therefore, a strategy involving effective encapsulation of a therapeutic payload is required to avoid mtDNA-related inflammation. Herein, we developed an in situ injectable chitosan hydrogel (ICH) loaded with lipid-BAK1 siRNA nanoparticle complexes that attenuate an increase of mtDNA. ATDC5 cells or bone marrow monocytes under inflammation stimulation were cocultured with siBAK@Lips@ICH. This hydrogel delivers lipid-BAK1 siRNA-loaded liposomes which downregulate BAK1 secretion and decrease mtDNA release, and this contributes significantly to upregulation of cGAS-STING-mediated inflammation. A knee osteolysis model was then established and treated with siBAK@Lips@ICH. Consequently, our data show that the delivery system provides comprehensive protection of cartilage and subchondral bone by ameliorating the whole process of inflammation reversal and amplification.

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An extracellular matrix-inspired self-healing composite hydrogel for enhanced platelet-rich plasma-mediated chronic diabetic wound treatment

Cited by 19 publications

References 58 publications

Enhanced wound regeneration by PGS/PLA fiber dressing containing platelet-rich plasma: an in vitro study

Enhanced wound regeneration by PGS/PLA fiber dressing containing platelet-rich plasma: an in vitro study

Engineering a platelet-rich plasma-based multifunctional injectable hydrogel with photothermal, antibacterial, and antioxidant properties for skin regeneration

Injectable Hydrogel Containing RNA-Loaded Liposomes Alleviates Chondrocyte Inflammation by Disrupting BAK1-Mediated mtDNA Maintenance

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