An extensive study of the genetic diversity within seven French wine grape variety collections

Pelsy, Frédérique; Hocquigny, Stéphanie; Moncada, Ximena; Barbeau, Gérard; Forget, Dominique; Hinrichsen, Patricio; Merdinoglu, Didier

doi:10.1007/s00122-009-1250-8

Cited by 83 publications

(60 citation statements)

References 44 publications

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“…The SSR analysis in current study has enabled to differentiate rootstock varieties (SO4, Ramsey and Dog Ridge) from other studied varieties. This supports the efficacy of SSR technique to differentiate and study the genetic relationship in Tunisian, Spanish, and French grapes (Zoghlami et al, 2009;Moreno-Sanz et al, 2011;Pelsy et al, 2010). The power of SSR technique was most effective to characterize all tested grape varieties through the production of unique positive and/or negative markers (Table 2).…”

Section: The Polymorphism Analysis Of Ssr Markerssupporting

confidence: 67%

Genetic diversity, variety identification and gene detection in some Egyptian grape varieties by SSR and SCoT markers

Ibrahim¹,

Adawy²,

Atia³

et al. 2016

Plant Omics

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Leaves of seven Egyptian grape varieties represent different range of agronomic and morphological traits were genotyped using Start-codon Targeted (SCoT) and Simple Sequence Repeats (SSR). The 24 SCoT primers generated 362 total fragments with 77.10% of polymorphism and 0.04 of average PIC. Dice coefficient that shows the genetic similarity and relationship was also used between the seven varieties. On one hand, SCoT analysis successfully characterized 73 unique positive and negative markers differentiating between the rootstock varieties especially those with green and red fruits. On the other hand, the seven SSR primers produced 73 fragments with 86.30% total polymorphism and 0.14 of average PIC. It also provided 19 unique positive and negative markers differentiating between the rootstock varieties. SO4 variety was identified by the highest number of positive unique markers (5). Both Scot and SSR analysis had covered 0.02% (0.10 Mbp) of V.vinifera genome. The SCoT covered 0.96 Mbp and SSR covered 846 bp. Together, the covered region encompassed about 22 genes of grape genome. Further, eight SCoT polymorphic bands were purified, cloned and sequenced. Of which, four SCoT sequences (SCoT3 600 , SCoT4 450 , SCoT6 200 and SCoT12 550 ) showed high similarity to some potential genes.

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Section: The Polymorphism Analysis Of Ssr Markerssupporting

confidence: 67%

Genetic diversity, variety identification and gene detection in some Egyptian grape varieties by SSR and SCoT markers

Ibrahim¹,

Adawy²,

Atia³

et al. 2016

Plant Omics

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“…Pelsy et al (2010) reported that, traditionally, clones are attributed to a variety on the basis of several common ampelographic traits, although phenotypic variations between clones of the same variety are often observed and accepted.…”

Section: Discussionmentioning

confidence: 99%

Ampelographic and Molecular Characterisation of Aglianico Accessions (Vitis vinifera L.) Collected in Southern Italy

Alba

Anaclerio

Gasparro³

et al. 2016

SAJEV

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To characterise 31 different Aglianico accessions randomly collected in Southern Italy, 30 ampelographic descriptors, 13 SSRs and 10 AFLP primer combinations were analysed. An appreciable variation of ampelographic descriptors was revealed mainly by mature leaf traits, while very few variations were recorded for shoot and berry traits. Similarly, all SSR loci revealed molecular monomorphism and AFLPs with a very high genetic similarity (Dice coefficient) among all the accessions considered. One of the aims of this study was to clarify the genetic assessment of Aglianico Nero and Aglianico del Vulture Nero, since they are registered as two different cultivars with distinct varietal codes at the Italian Register of Grape Varieties. Registered Aglianico Nero and Aglianico del Vulture Nero were included in the analyses, compared and used as reference material. Our plants showed that all the accessions tested, independent from the biotype, and the two registered cultivars belong to the same genotype, suggesting that, as reported by the Vitis International Variety Catalogue, a case of synonymy occurred between Aglianico Nero and Aglianico del Vulture Nero. These cultivars could therefore be considered as a single cultivar. Moreover, the AFLP data revealed a partial match between morphological and molecular data, showing that the AFLP molecular method was able to discriminate between different accessions belonging to the same cultivar.

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“…Among them only few hundred are cultivated for commercial wine production (Truel et al, 1980). The management of these collections requires attention to avoid redundancy, to track introductions that were wrongly assigned to a cultivar and to assist clone selection (Pelsy et al, 2010). So, identification of the plant material is crucial and represents the first step in germplasm management (Cipriani et al, 2010;Laucou et al, 2011).…”

Section: Dna Isolation Rapd and Issr Amplificationmentioning

confidence: 99%

A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

Castro

Pinto-Carnide

Ortiz

et al. 2017

Span. j. agric. res.

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ance of millions of hectares produced by the attack of that insect. More recently, genetic diversity has suffered another drawback. New plantations with foreign material with low genetic variability have reinforced genetic erosion of native germplasm. Moreover, European Union incentives for restructuring and conversion, particularly in Portugal and Spain, have conducted to RESEARCH ARTICLE OPEN ACCESS AbstractGrapevine cultivars diversity is vast and full of synonyms and homonyms. Up to few decades ago characterization of grapevine was based on morphological characters. In the last decades, molecular markers were developed and have been used as tools to study genetic diversity in a range of different plant species. Fifty-six Portuguese accessions representative of 'Vinhos Verdes' and 'Douro' Controlled Designations of Origin (DOC) were analysed through DNA fingerprints generated by Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR). The study aimed to compare the effectiveness of RAPD and ISSR molecular techniques in the detection of synonyms, homonyms and misnames. RAPD and ISSR analysis enabled the detection of 36 different band patterns, reducing in about 36% the initial material. Several accessions grown under different names, between and within collections, were confirmed as the same genotype, namely Gouveio/Verdelho, Sousão Douro/Vinhão and Arinto Oeste/ Pedernã. Similarly, some homonyms/misnames were also identified, namely within Azal Tinto and Rabigato accessions. RAPD and ISSR markers revealed to be adequate molecular techniques for grapevine varieties fingerprinting with advantages over other molecular procedures, contributing for a good management of grapevine collections.

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An extensive study of the genetic diversity within seven French wine grape variety collections

Cited by 83 publications

References 44 publications

Genetic diversity, variety identification and gene detection in some Egyptian grape varieties by SSR and SCoT markers

Genetic diversity, variety identification and gene detection in some Egyptian grape varieties by SSR and SCoT markers

Ampelographic and Molecular Characterisation of Aglianico Accessions (Vitis vinifera L.) Collected in Southern Italy

A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

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