An Extended Anaphase Signaling Pathway for Mad2p Includes Microtubule Organizing Center Proteins and Multiple Motor-dependent Transitions

Mayer, Christina; Filopei, Jason; Batac, Joe; Alford, Lea M.; Paluh, Janet L.

doi:10.4161/cc.5.13.2912

Cited by 22 publications

(27 citation statements)

References 45 publications

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“…Thus, Pkl1p plays no detectable role in deactivating the checkpoint via the reduction of Mad2 levels during prometaphase. In anaphase, Mad2-GFP was seen as weak dots at one or both ends of the spindle (Ikui et al, 2002;Garcia et al, 2002;Saitoh et al, 2005), and unlike a previous report (Mayer et al, 2006), this pattern was unaffected by the absence of Pkl1p ( Figure 2D). Furthermore, only 1 of 17 anaphase cells with unequal daughter nuclei (such as in Figure 1E) exhibited a relatively bright Mad2-GFP signal in the larger nucleus.…”

Section: Pkl1p Does Not Play a Direct Role In The Mad2-dependent Checcontrasting

confidence: 44%

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Mitotic Chromosome Biorientation in Fission Yeast Is Enhanced by Dynein and a Minus-end–directed, Kinesin-like Protein

Grishchuk

Spiridonov

McIntosh

2007

MBoC

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Chromosome biorientation, the attachment of sister kinetochores to sister spindle poles, is vitally important for accurate chromosome segregation. We have studied this process by following the congression of pole-proximal kinetochores and their subsequent anaphase segregation in fission yeast cells that carry deletions in any or all of this organism's minus end-directed, microtubule-dependent motors: two related kinesin 14s (Pkl1p and Klp2p) and dynein. None of these deletions abolished biorientation, but fewer chromosomes segregated normally without Pkl1p, and to a lesser degree without dynein, than in wild-type cells. In the absence of Pkl1p, which normally localizes to the spindle and its poles, the checkpoint that monitors chromosome biorientation was defective, leading to frequent precocious anaphase. Ultrastructural analysis of mutant mitotic spindles suggests that Pkl1p contributes to error-free biorientation by promoting normal spindle pole organization, whereas dynein helps to anchor a focused bundle of spindle microtubules at the pole.

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Section: Pkl1p Does Not Play a Direct Role In The Mad2-dependent Checcontrasting

confidence: 44%

“…A direct study of chromosome poleward motion during mitotic prometaphase has revealed little or no abnormality when either Dhc1p or Pkl1p was absent . Late in mitotic division dynein and Pkl1p may be involved in localizing the checkpoint protein Mad2p to the spindle midzone (Mayer et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Mitotic Chromosome Biorientation in Fission Yeast Is Enhanced by Dynein and a Minus-end–directed, Kinesin-like Protein

Grishchuk

Spiridonov

McIntosh

2007

MBoC

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“…In fission yeast, interactions between Mad2 and the g-tubulin associated protein Alp4 were found to serve as a sensor for spindle function. 57 When this interaction is defective or lost, cells interpreted this as a defect in spindle assembly. 57 This finding is particularly interesting as it suggests that Mad2-Alp4 interactions are monitored by the checkpoint machinery and required for mitotic exit, which is in contrast to a previous study that demonstrates mitotic exit to be independent of these interactions.…”

Section: Mtocs and The Checkpoint Responsementioning

confidence: 99%

Control At The Cell Center: The Role Of Spindle Poles In Cytoskeletal Organization And Cell Cycle Regulation

Cuschieri¹,

Nguyen²,

Vogel³

2007

Cell Cycle

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“…Previous studies have suggested that the onset of asymmetry of Cdc7p-GFP in anaphase B is delayed by the presence of unsegregated DNA (Mayer et al, 2006). To investigate this further, we examined mutants in which lagging chromosomes are observed on an extended anaphase spindle.…”

Section: The Spindle Assembly Checkpoint Affects Cdc7p-gfp Spb Associmentioning

confidence: 99%

Analysis ofS. pombeSIN protein SPB-association reveals two genetically separable states of the SIN

Wachowicz

Chasapi

Krapp

et al. 2014

Journal of Cell Science

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The Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis, and asymmetric association of SIN proteins with the mitotic spindle pole bodies (SPBs) is important for its regulation. Here, we have used semi-automated image analysis to study SIN proteins in large numbers of wild-type and mutant cells. Our principal conclusions are: first, that the association of Cdc7p with the SPBs in early mitosis is frequently asymmetric, with a bias in favour of the new SPB; second, that the early association of Cdc7p-GFP to the SPB depends on Plo1p but not Spg1p, and is unaffected by mutations that influence its asymmetry in anaphase; third, that Cdc7p asymmetry in anaphase B is delayed by Pom1p and by activation of the spindle assembly checkpoint, and is promoted by Rad24p; and fourth, that the length of the spindle, expressed as a fraction of the length of the cell, at which Cdc7p becomes asymmetric is similar in cells dividing at different sizes. These data reveal that multiple regulatory mechanisms control the SIN in mitosis and lead us to propose a two-state model to describe the SIN.

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An Extended Anaphase Signaling Pathway for Mad2p Includes Microtubule Organizing Center Proteins and Multiple Motor-dependent Transitions

Cited by 22 publications

References 45 publications

Mitotic Chromosome Biorientation in Fission Yeast Is Enhanced by Dynein and a Minus-end–directed, Kinesin-like Protein

Mitotic Chromosome Biorientation in Fission Yeast Is Enhanced by Dynein and a Minus-end–directed, Kinesin-like Protein

Control At The Cell Center: The Role Of Spindle Poles In Cytoskeletal Organization And Cell Cycle Regulation

Analysis ofS. pombeSIN protein SPB-association reveals two genetically separable states of the SIN

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