An extendable all-in-one injection twin derivatization LC-MS/MS strategy for the absolute quantification of multiple chemical-group-based submetabolomes

YeZhen, Huang; Jiao, Yu; Gao, Yiqiao; Zhang, Pei; Huang, Wanqiu; Liu, Yong; Huang, Yin; Tian, Yuan; Wan, Jian‐Bo; Zhang, Zunjian; Hao, Kun; Xu, Fengguo

doi:10.1016/j.aca.2019.02.001

Cited by 23 publications

(12 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since peak interference was present in this scenario, while using formic acid could be an acceptable alternative. Similarly, Xu et al exploited this strategy using Dns-PP/Dens-PP quantitative profiling of eicosanoids derived from n-6 and n-3 polyunsaturated fatty acids, FFAs and multiple chemical-group-based sub-metabolomes (17,29,30). These methods not only improved the LC separation of the targets, but also enhanced the ion MS response.…”

Section: Discussionmentioning

confidence: 99%

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Zhang

Yang

Wang

et al. 2021

Journal of Lipid Research

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: Discussionmentioning

confidence: 99%

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Zhang

Yang

Wang

et al. 2021

Journal of Lipid Research

View full text Add to dashboard Cite

show abstract

“…Amino/phenol group containing metabolites were reacted with Dns-Cl, and carboxyl group containing metabolites were labeled by Dns-PP prior to the LC-MS/MS analysis (detailed information on the classification of metabolites is provided in Supporting Information Table S1). The chemical derivatization reaction was performed according to our previous study with some modifications …”

Section: Materials and Methodsmentioning

confidence: 99%

“…The chemical derivatization reaction was performed according to our previous study with some modifications. 30 2.4.1.1. Dns-Cl Derivatization.…”

Section: Methodsmentioning

confidence: 99%

Tryptophan Pathway-Targeted Metabolomics Study on the Mechanism and Intervention of Cisplatin-Induced Acute Kidney Injury in Rats

Tan

Chen

Qin

et al. 2021

Chem. Res. Toxicol.

Self Cite

View full text Add to dashboard Cite

Cisplatin is a chemotherapeutic agent widely employed in the treatment of various solid tumors. However, its use is often restricted by acute kidney injury (AKI) which is the dose-limiting adverse effect of cisplatin. While numerous studies aiming to alleviate the AKI have been conducted, there are no effective remedies in clinical practice. In this paper, a targeted metabolomics study was performed to reveal the potential relationship between tryptophan metabolism and cisplatin-induced AKI. A chemical derivatization integrated liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) approach was utilized to quantify 29 metabolites in the tryptophan pathway in rat kidney medulla and cortex after cisplatin administration. Results showed that tryptophan metabolism was remarkably disturbed both in the medulla and cortex after cisplatin administration. We also found that the tryptophan pathway in the medulla was more sensitive to cisplatin exposure compared with the cortex. Among these metabolites, indoxyl sulfate was focused for further study because it accumulated most significantly in the kidney cortex and medulla in a dose-dependent manner. A function verification study proved that chlormethiazole, a widely used CYP2E1 inhibitor, could reduce the production of indoxyl sulfate in the liver and attenuate cisplatin-induced AKI in rats. In conclusion, our study depicted the tryptophan pathway in cisplatin-induced AKI for the first time and demonstrated tryptophan metabolism is closely associated with the renal toxicity caused by cisplatin, which can be of great use for the discovery of renal toxicity attenuating remedies.

show abstract

“…Stable isotope labeling (SIL) is a commonly used strategy in LC-MS to improve metabolite determination. Especially in LC-MS-based metabolomic studies, SIL derivatization reagents are often used to react with different substituents (including carboxyl, carbonyl, amino, and sulfhydryl) to rapidly identify different kinds of metabolites. − But so far, such similar strategies have not been applied in MALDI MSI studies.…”

Section: Introductionmentioning

confidence: 99%

Quaternized Acridine Maleimide MALDI Probe Enables Mass Spectrometry Imaging of Thiols

Yuan

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

Thiols are essential metabolites associated with redox imbalances and metabolic disorders in diseases. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) facilitates imaging of metabolites in tissue, but imaging of thiols remains challenging. Here we developed a method to visualize thiols using a stable isotope-labeled (SIL) MALDI probe, a mixture of unlabeled and deuterium-labeled reagents that provided adduct signals at [M] + and [M + 3] + , to identify endogenous thiols in tissue. A series of MALDI probe candidates were rationally designed, and the structure−effect relationships were determined. First, the reactivity of different warheads toward the thiol group was evaluated, and maleimide was the best for in situ derivatization. Second, an acridine fragment showed the best improvement in MS responses. Third, a permanent charge was introduced for detection improvement in the positive mode. Finally, the hydrogens of methyl group were replaced by deuterium atoms, obtaining the novel SIL MALDI probe and thus facilitating significantly the annotation of thiols. The finally obtained D 0 /D 3 -9-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)-10-methylacridin-10-ium iodide (D 0 /D 3 -MaI-MADA) enabled direct MSI of thiols in the fine structures of human liver tumors without a reduction procedure. Our work built a SIL MALDI probe for the first time and provided a strategy for the rational design of MALDI probes.

show abstract

An extendable all-in-one injection twin derivatization LC-MS/MS strategy for the absolute quantification of multiple chemical-group-based submetabolomes

Cited by 23 publications

References 28 publications

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Tryptophan Pathway-Targeted Metabolomics Study on the Mechanism and Intervention of Cisplatin-Induced Acute Kidney Injury in Rats

Quaternized Acridine Maleimide MALDI Probe Enables Mass Spectrometry Imaging of Thiols

Contact Info

Product

Resources

About