An exploratory study on the efficacy of rat dedifferentiated fat cells (rDFATs) with a poly lactic-co-glycolic acid/hydroxylapatite (PLGA/HA) composite for bone formation in a rat calvarial defect model

Shirakata, Yoshinori; Nakamura, Toshiaki; Shinohara, Yukiya; Taniyama, Katsuyoshi; Sakoda, Kenji; Yoshimoto, Takehiko; Noguchi, Kazuyuki

doi:10.1007/s10856-013-5124-x

Cited by 18 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mature adipocytes begin to dedifferentiate into elongated non-lipid-filled fibroblast-like cells when cultured in specific conditions such as by using ceiling culture technique [ 4 ] and hydrogels [ 5 ] in vitro . The cells differentiate into multiple cell lineages, including osteoblasts [ 3 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ], chondrocytes [ 3 , 14 , 16 ], adipocytes [ 3 , 15 , 17 ], myocytes [ 14 , 15 , 18 , 19 , 20 ], and endothelial cells [ 21 ]. Expression markers and proliferative capacity of DFAT cells are analogous to those of MSCs and ADSCs, but are more homogenous [ 3 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro

Kaida

Honda

Hashimoto

et al. 2015

IJMS

View full text Add to dashboard Cite

Despite advances in stem cell biology, there are few effective techniques to promote the osteogenic differentiation of human primary dedifferentiated fat (DFAT) cells. We attempted to investigate whether epigallocatechin-3-gallate (EGCG), the main component of green tea catechin, facilitates early osteogenic differentiation and mineralization on DFAT cells in vitro. DFAT cells were treated with EGCG (1.25–10 μM) in osteogenic medium (OM) with or without 100 nM dexamethasone (Dex) for 12 days (hereafter two osteogenic media were designated as OM(Dex) and OM). Supplementation of 1.25 μM EGCG to both the media effectively increased the mRNA expression of collagen 1 (COL1A1) and runt-related transcription factor 2 (RUNX2) and also increased proliferation and mineralization. Compared to OM(Dex) with EGCG, OM with EGCG induced earlier expression for COL1A1 and RUNX2 at day 1 and higher mineralization level at day 12. OM(Dex) with 10 μM EGCG remarkably hampered the proliferation of the DFAT cells. These results suggest that OM(without Dex) with EGCG might be a preferable medium to promote proliferation and to induce osteoblast differentiation of DFAT cells. Our findings provide an insight for the combinatory use of EGCG and DFAT cells for bone regeneration and stem cell-based therapy.

show abstract

Section: Introductionmentioning

confidence: 99%

Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro

Kaida

Honda

Hashimoto

et al. 2015

IJMS

View full text Add to dashboard Cite

show abstract

“…And it is known that PLGA is inadequate for cell attachment and proliferation 20,21) . There are many factors about surface adsorption of biomaterials, contact angle is often used as an indicator of them.…”

Section: Discussionmentioning

confidence: 99%

Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium

et al. 2017

View full text Add to dashboard Cite

In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells.

show abstract

“…In the first study that reported the ceiling culture method, a 0.2% collagenase solution was used to isolate mature adipocytes (24). However, other researchers have shown that 0.1% (6), 0.3% (10), 0.2% (25), and 0.075% (26) collagenase can also be used. To the best of our knowledge, there are no studies defining the optimal concentration of collagenase for isolating small adipocytes from BFPs.…”

Section: Effect Of Collagenase Concentration On the Isolation Of Smalmentioning

confidence: 99%

Effect of collagenase concentration on the isolation of small adipocytes from human buccal fat pad

et al. 2018

View full text Add to dashboard Cite

Dedifferentiated fat (DFAT) cells were isolated from mature adipocytes using the ceiling culture method. Recently, we successfully isolated DFAT cells from adipocytes with a relatively small size (<40 μm). DFAT cells have a higher osteogenic potential than that of medium adipocytes. Therefore, the objective of this study was to determine the optimal concentration of collagenase solution for isolating small adipocytes from human buccal fat pads (BFPs). Four concentrations of collagenase solution (0.01%, 0.02%, 0.1%, and 0.5%) were used, and their effectiveness was assessed by the number of small adipocytes and DFAT cells isolated. The total number of floating adipocytes that dissociated with 0.02% collagenase was 2.5 times of that dissociated with 0.1% collagenase. The number of floating adipocytes with a diameter of ≤29 μm that dissociated with 0.02% collagenase was thrice of those dissociated with 0.1% and 0.5% collagenase. The number of DFAT cells that dissociated with 0.02% collagenase was 1.5 times of that dissociated with 0.1% collagenase. In addition, DFAT cells that dissociated with 0.02% collagenase had a higher osteogenic differentiation potential than those that dissociated with 0.1% collagenase. These results suggest that 0.02% is the optimal collagenase concentration for isolating small adipocytes from BFPs.

show abstract

An exploratory study on the efficacy of rat dedifferentiated fat cells (rDFATs) with a poly lactic-co-glycolic acid/hydroxylapatite (PLGA/HA) composite for bone formation in a rat calvarial defect model

Cited by 18 publications

References 26 publications

Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro

Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro

Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium

Effect of collagenase concentration on the isolation of small adipocytes from human buccal fat pad

Contact Info

Product

Resources

About