An exploratory study on isochoric supercooling preservation of the pig liver

Botea, Florin; Năstase, Gabriel; Herlea, Vlad; Chang, Tzu-Ming; Şerban, Alexandru; Bârcu, Alexandru; Rubinsky, Boris; Popescu, Irinel

doi:10.1016/j.bbrep.2023.101485

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…Finally, the work developed by Rubinsky and his team on isochoric (constant volume) supercooling showed successful supercooling in porcine livers for up to 48 h at −2°C ( 39 ), taking up the challenge of subzero non-freezing state in a large organ. Their team also developed a system for cryomicroscopy, making it possible to study biological systems at subzero temperatures and understand the cell behavior toward ice formation ( 40 ).…”

Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 99%

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Berkane,

Hayau,

Filz von Reiterdank

et al. 2023

Front. Transplant.

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Ex vivo preservation of transplanted organs is undergoing spectacular advances. Machine perfusion is now used in common practice for abdominal and thoracic organ transportation and preservation, and early results are in favor of substantially improved outcomes. It is based on decreasing ischemia-reperfusion phenomena by providing physiological or sub-physiological conditions until transplantation. Alternatively, supercooling techniques involving static preservation at negative temperatures while avoiding ice formation have shown encouraging results in solid organs. Here, the rationale is to decrease the organ's metabolism and need for oxygen and nutrients, allowing for extended preservation durations. The aim of this work is to review all advances of supercooling in transplantation, browsing the literature for each organ. A specific objective was also to study the initial evidence, the prospects, and potential applications of supercooling preservation in Vascularized Composite Allotransplantation (VCA). This complex entity needs a substantial effort to improve long-term outcomes, marked by chronic rejection. Improving preservation techniques is critical to ensure the favorable evolution of VCAs, and supercooling techniques could greatly participate in these advances.

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Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 99%

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Berkane,

Hayau,

Filz von Reiterdank

et al. 2023

Front. Transplant.

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Experimental studies of semiautomatic cryoconservation process for biological tissue specimens

Pushkarev,

Burkov,

Ivannikova

et al. 2024

Biomed Eng

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Liquid–solid equilibria and supercooling of Custodiol® in isochoric thermodynamic systems at subfreezing temperatures

Câmpean,

Beșchea,

Tăbăcaru

et al. 2023

Physics of Fluids

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There is growing interest in using isochoric freezing and isochoric supercooling for the preservation of biological matter at subfreezing temperatures. Custodiol® is a commonly used intracellular composition type, subnormothermic preservation solution. It is anticipated that Custodiol® will also be used for isochoric freezing and isochoric supercooling preservation of biological matter. The thermodynamic properties of Custodiol® at subfreezing temperatures as well as the metastable behavior of the solution at subfreezing temperatures were not studied in the past. This study was designed to generate the thermodynamic data needed for the use of Custodiol® for the preservation of biological matter in isochoric systems at subfreezing temperatures. The experiments were performed in a specially designed isochoric chamber that can measure simultaneously the temperature and pressure in the isochoric chamber, and thereby correlate pressure and temperature at thermodynamic equilibrium in isochoric systems as well as the nucleation temperature in isochoric supercooling. The primary focus of this study is on determining the temperature at which nucleation is initiated and to identify the temperature threshold for nucleation due to its specific relevance to various applications in medicine.

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An exploratory study on isochoric supercooling preservation of the pig liver

Cited by 3 publications

References 38 publications

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Experimental studies of semiautomatic cryoconservation process for biological tissue specimens

Liquid–solid equilibria and supercooling of Custodiol® in isochoric thermodynamic systems at subfreezing temperatures

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