An exploratory investigation of various modes of action and potential adverse outcomes of fluoxetine in marine mussels

Franzellitti, Silvia; Buratti, Sara; Capolupo, Marco; Du, Bowen; Haddad, Samuel P.; Chambliss, C. Kevin; Brooks, Bryan W.; Fabbri, Elena

doi:10.1016/j.aquatox.2013.11.016

Cited by 113 publications

(61 citation statements)

References 89 publications

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“…In more recent laboratory studies, Hazelton et al [41] observed fluoxetine BAFs for a unionid mussel to range between 229 and 1221. Elevated SSRI levels observed in bivalves (table 1) during this study may have resulted from partitioning to lysosomes [42]. Additionally, SSRIs and other pharmaceuticals, which are weakly basic and having an amino group, have been reported to be effectively sequestered by lysosomes of mammalian cells, resulting in drug accumulation [43].…”

Section: (D) Bioaccumulation Of Human Pharmaceuticals By Various Aquamentioning

confidence: 99%

Bioaccumulation and trophic dilution of human pharmaceuticals across trophic positions of an effluent-dependent wadeable stream

Haddad

Luek

et al. 2014

Phil. Trans. R. Soc. B

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131

118

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Though pharmaceuticals are increasingly observed in a variety of organisms from coastal and inland aquatic systems, trophic transfer of pharmaceuticals in aquatic food webs have not been reported. In this study, bioaccumulation of select pharmaceuticals was investigated in a lower order effluent-dependent stream in central Texas, USA, using isotope dilution liquid chromatography–tandem mass spectrometry (MS). A fish plasma model, initially developed from laboratory studies, was tested to examine observed versus predicted internal dose of select pharmaceuticals. Pharmaceuticals accumulated to higher concentrations in invertebrates relative to fish; elevated concentrations of the antidepressant sertraline and its primary metabolite desmethylsertraline were observed in the Asian clam, Corbicula fluminea , and two unionid mussel species. Trophic positions were determined from stable isotopes (δ 15 N and δ 13 C) collected by isotope ratio-MS; a Bayesian mixing model was then used to estimate diet contributions towards top fish predators. Because diphenhydramine and carbamazepine were the only target compounds detected in all species examined, trophic magnification factors (TMFs) were derived to evaluate potential trophic transfer of both compounds. TMFs for diphenhydramine (0.38) and carbamazepine (1.17) indicated neither compound experienced trophic magnification, which suggests that inhalational and not dietary exposure represented the primary route of uptake by fish in this effluent-dependent stream.

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Section: (D) Bioaccumulation Of Human Pharmaceuticals By Various Aquamentioning

confidence: 99%

Bioaccumulation and trophic dilution of human pharmaceuticals across trophic positions of an effluent-dependent wadeable stream

Haddad

Luek

et al. 2014

Phil. Trans. R. Soc. B

Self Cite

131

118

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“…Moreover, M endez-Arriaga et al (2011), also stated that photolysis of fluoxetine is remarkable only under alkaline pH. A rapid uptake of fluoxetine via waterborne exposure across the gills of mussels, presumably through feeding and respiratory activities, probably took place (Franzellitti et al, 2014). Detectable levels of fluoxetine were also observed by Franzellitti et al (2014) Bringolf et al (2010) also observed that, only after 14 days of exposure, caged mussels at stream sites near a municipal WWTP effluent discharge accumulated substantial quantities of fluoxetine in their tissues.…”

Section: Frequency Uptake and Metabolization By M Galloprovincialismentioning

confidence: 99%

“…Since then, some studies reported fluoxetine accumulation in fish tissues (Silva et al, 2015) and fewer on refer other aquatic organisms, such as mussels (Bringolf et al, 2010;Franzellitti et al, 2014;Maruya et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, dietary routes of fluoxetine exposure and uptake may be particularly important for bivalves (Franzellitti et al, 2014). Bivalves are filter feeders sessile organisms which draw in water and particles from their surrounding environment .…”

Section: Introductionmentioning

confidence: 99%

“…Bivalves are filter feeders sessile organisms which draw in water and particles from their surrounding environment . There is still a paucity of data regarding accumulation and metabolization of pharmaceuticals, such as fluoxetine, by bivalves and these mechanisms deserve additional clarification (Boxall et al, 2012;Franzellitti et al, 2014;Du et al, 2015). The blue mussel Mytilus galloprovincialis is generally considered a good bioindicator of environmental quality and of accumulation of numerous contaminants (Bebianno et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Uptake, accumulation and metabolization of the antidepressant fluoxetine by Mytilus galloprovincialis

Silva

Martins

Pereira

et al. 2016

Environmental Pollution

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a b s t r a c tFluoxetine, a selective serotonin re-uptake inhibitor (SSRI) antidepressant, is among the most prescribed pharmaceutical active substances worldwide. This study aimed to assess its accumulation and metabolization in the mussel Mytillus galloprovincialis, considered an excellent sentinel species for traditional and emerging pollutants. Mussels were collected from Ria Formosa Lagoon, Portugal, and exposed to a nominal concentration of fluoxetine (75 ng L À1 ) for 15 days. Approximately 1 g of whole mussel soft tissues was extracted with acetonitrile:formic acid, loaded into an Oasis MCX cartridge, and fluoxetine analysed by liquid chromatography with tandem mass spectrometry (LC-MSn). After 3 days of exposure, fluoxetine was accumulated in 70% of the samples, with a mean of 2.53 ng g À1 dry weight (d.w.) and norfluoxetine was only detected in one sample (10%), at 3.06 ng g À1 d.w. After 7 days of exposure, the accumulation of fluoxetine and norfluoxetine increased up to 80 and 50% respectively, and their mean accumulated levels in mussel tissues were up to 4.43 and 2.85 ng g À1 d.w., respectively. By the end of the exposure period (15 days), both compounds were detected in 100% of the samples (mean of 9.31 and 11.65 ng g À1 d.w., respectively). Statistical analysis revealed significant accumulation differences between the 3rd and 15th day of exposure for fluoxetine, and between the 3rd and 7th against the 15th day of exposure for norfluoxetine. These results suggest that the fluoxetine accumulated in mussel tissues is likely to be metabolised into norfluoxetine with the increase of the time of exposure, giving evidence that at these realistic environmental concentrations, toxic effects of fluoxetine in mussel tissues may occur.

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Mussels and Marine Environmental Contaminants

2021

Marine Mussels

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An exploratory investigation of various modes of action and potential adverse outcomes of fluoxetine in marine mussels

Cited by 113 publications

References 89 publications

Bioaccumulation and trophic dilution of human pharmaceuticals across trophic positions of an effluent-dependent wadeable stream

Bioaccumulation and trophic dilution of human pharmaceuticals across trophic positions of an effluent-dependent wadeable stream

Uptake, accumulation and metabolization of the antidepressant fluoxetine by Mytilus galloprovincialis

Mussels and Marine Environmental Contaminants

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