This study aims to partially or simultaneously observe the influence of educational factors, environmental factors, and internet service availability factors on student interest in practicing online business. This study employed a survey method. The obtained sample of this study included 94 respondents, selected by purposive sampling method from the population of all study programs. Research information data was collected through a questionnaire instrument. Data analysis was performed through multiple regression technique. The results of this study indicate that partially variable education and availability of internet services had a significant effect on online business. However, environmental variables had no significant effect on online business. Simultaneously, the variables of education, environment, and availability of internet services had a significant effect on online business interests.