An exploratory analysis on the toxicity & safety profile of Polyherbal combination of curcumin, quercetin and rutin

Abstract

Background Curcumin, quercetin and rutin are flavonoids having strong antioxidant potential, individually used in treatment of numerous ailments. The safety assessment of each of them is already established but no toxicological assessment has been done that would guarantee the safe use of these three flavonoids when used as a polyherbal combination (PHC). The aim of this study to evaluate the possible toxicological effect of polyherbal combination of these three flavonoids in female Swiss albino mice. Methods In acute toxicity study, the oral dose of poly herbal combination was administered to four groups stepwise in single dose and general behaviour, adverse effects and mortality were determined up to 14 days and compared to normal group. In sub-acute study, the tested poly herbal combination was administered orally for 28 days to the four experimental groups and their body weight was measured each alternate day from the first day of dosing. On 29th day the final body weight was recorded and euthanized by using thiopentone sodium, blood was collected and later haematological, lipid profile, biochemical parameter was evaluated and compared to normal group. Result In acute toxicity study, no abnormal general behaviour, adverse effects were reported. No significant changes were reported in body weight, haematological, lipid profile, biochemical parameter in sub-acute toxicity study. No mortality was reported in both the study. Histopathological examination revealed no alterations in clinical signs or organ weight at any dose. Conclusion The result concludes that the oral administration of Polyherbal combination did not produce any significant toxic effect in swiss albino mice. Hence, the Polyherbal combination can be utilized safely for therapeutic use.

Quercetin‐loaded chitosan nanoparticles as an alternative for controlling bacterial adhesion to urethral catheter

“…showed that quercetin seems to be safe at a dose level of 2000 mg/kg, and the LD50 is considered to be >2000 mg/kg. Any pharmaceutical drug or compound with an oral LD50 higher than 1000 mg/kg could be considered safe 8,9 . Thus, data from the literature supports the safe use of quercetin in the concentration proposed in this study.…”

“…It is abundant in foods such as fruits and vegetables. The in vivo and in vitro genotoxicity, acute, subchronic, and chronic toxicity, and carcinogenicity studies of quercetin exposed that at predictable dietary intake levels, it does not result in adverse health effects 8,9 . Recently, Tiwari et al 9 .…”

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“…(1) Enhances glucose uptake through the activation of AMPK signaling pathway in skeletal muscle cells [61,62] (2) Protects the β-cells from apoptosis [63] (3) Enhances insulin receptor beta-subunit, GLUT4, and tissue insulin sensitivity [64] (4) Inhibitory effect on α-glucosidases [65] (5) Inhibits gluconeogenesis through the upregulation of AMPK [66] Sambucus nigra L. [67] Madagascar periwinkle [68] Catharanthus roseus [68] Elaeodendron croceum [68] Fisetin…”

“…It is reported that rutin can alter Ca 2+ uptake in isolated pancreatic islets, followed by an increase in the uptake of Ca 2+ . Tiwari et al have documented those previous studies that showed rutin is safe at a concentration of 2000 mg/kg and the intake of rutin from dietary sources will not cause any toxicity effects [ 61 ]. Rutin is widely distributed in plants, for example, Ruta graveolens , Morus alba , asparagus, and buckwheat [ 62 ].…”