An explanation of the mineralization mechanism in osteoblasts induced by calcium hydroxide

Narita, Hiroko; Itoh, Shousaku; Imazato, Satoshi; Yoshitake, Fumio; Ebisu, Shigeyuki

doi:10.1016/j.actbio.2009.08.005

Cited by 44 publications

(50 citation statements)

References 26 publications

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“…But it cannot be ignored that the OH -might have an impact on the CMS. The OHincreases the pH of the local environment [3,5]; hence the increment of pH could have an effect on the conditions that were used to evaluate the CMS. At the same time, the degradation of PLGA promotes an acidification of the local environment that might impact on the alkalinity of the medium [23], but OH -could reduce such impact.…”

Section: Discussionmentioning

confidence: 99%

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Sustained release of calcium hydroxide from poly(dl-lactide-co-glycolide) acid microspheres for apexification

et al. 2015

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Calcium hydroxide (CH) loaded poly(DL-lactide-co-glycolide) acid (PLGA) microspheres (MS) might be used for apexification requiring a sustained release of Ca(2+). The aim of this study was to formulate and characterize CH-PLGA-MS. The CH-loaded MS were prepared by either oil-in-water (O/W) or water-in-oil/in-water (W/O/W) emulsion solvent evaporation technique. MS produced by the O/W technique exhibited a larger diameter (18.63 ± 7.23 μm) than the MS produced by the W/O/W technique (15.25 ± 7.37 μm) (Mann-Whitney U test P < 0.001). The CH encapsulation efficiency (E e) and Ca(2+) release were calculated from data obtained by absorption techniques. Ca(2+) release profile was evaluated for 30 days. To know the E e, the CH-loaded MS were dissolved in 1 M NaOH to release all its content and a Ca(2+) colorimetric marker was added to this solution. The reagent marked the Ca(2+) in blue color, which was then measured by a UV-Vis system (650 nm). The percentage of E e was calculated on the basis of the theoretical loading. The E e of the O/W-produced MS was higher (24 %) than the corresponding percentage of the W/O/W-produced MS (11 %). O/W- and W/O/W-produced MS released slower and lower Ca(2+) than a control CH paste with polyethylene glycol 400 (Kruskal-Wallis test). O/W-produced MS released higher Ca(2+) than W/O/W-produced MS (statistically significant differences; P < 0.05). In conclusion, the CH-PLGA-MS were successfully formulated; the technique of formulation influenced the size, encapsulation efficiency and release profile. The MS were better sustained release system than the CH paste.

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Section: Discussionmentioning

confidence: 99%

“…At the same time, the high alkaline environment and released calcium ions (Ca 2? ) induce cellular activity promoting mineral tissue formation [3][4][5]. Because the apexification is a long-term treatment, it requires sustained release of Ca 2?…”

Section: Introductionmentioning

confidence: 99%

Sustained release of calcium hydroxide from poly(dl-lactide-co-glycolide) acid microspheres for apexification

et al. 2015

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“…Large amounts of calcium hydroxide and calcium oxide are important to enhance the mineralization process that occurs during apical repair. 6 The pH level and calcium release are important chemical properties which are directly related to mineralization process 28,29,30 and antimicrobial activity. 28,31 The pH level analysis revealed that, within the first 3 hours, results were similar among CCPG, saline solution, 1% NaOCl gel and 2% CHX gel groups, and that there were statistically significant differences between the K-Y gel and th distilled water (control) group.…”

Section: Discussionmentioning

confidence: 99%

Effects of various additives on antimicrobial, physical and chemical properties of mineral trioxide aggregate (MTA)

Andrade¹,

Alcalde²,

GUIMARÃƒES³

et al. 2015

Dental Press Endod

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Objective: To assess the antimicrobial activity, solubility, setting time, flowability, pH, calcium release and surface characteristic of mineral trioxide aggregate (MTA) associated with different substances: 1% sodium hypochlorite gel, 2% chlorhexidine gel, K-Y gel, distilled water associated with 10% propylene glycol (CCPG), saline solution, and distilled water alone. Methods: The antimicrobial test included Streptococcus mutans, Lactobacillus casei, Enterococcus faecalis and Candida albicans, and the direct contact method was used. To assess setting time and flowability, ASTM 266/08 and ADA 57/2007 specifications were used. To assess pH and calcium release, the different types of material were inserted into retrograde cavities of acrylic resin teeth and immersed in ultrapure water for reading at different periods. The latter was performed with a pHmeter and an atomic absorption spectrophotometer. To assess the surface characteristics, acrylic teeth were analyzed under scanning electron micrograph (SEM). Results: The results yielded by the antimicrobial activity test revealed that additives associated with MTA were more inhibitory than bactericidal, with chlorhexidine achieving the best results. The K-Y vehicle made the additive more soluble than others. There were statistical differences amongst groups (p > 0.05) for flowability. The greatest setting time was observed in the CCPG group. The K-Y group presented the lowest pH and calcium release values in the 3-hour period. In the other periods, there was greater uniformity amongst groups. As regards the surface characteristics analysis, the CCPG group presented the greatest porosity (p < 0.05). Conclusions:Only the chlorhexidine gel brought some improvement to the antimicrobial effect, K-Y gel interfered in the physical-chemical properties of MTA and the addition of CCPG provided the greatest porosity.Keywords: Antimicrobial sensitivity test. Calcium release. Solubility. pH level. Physical-chemical properties. MTA.

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“…Bone mineralization was analyzed using the alizarin red staining method [11] . Cells were inoculated at a cell density of 1×10 5 in 6-well culture plates and co-cultured with β-TCP ceramics for 5 days.…”

Section: Alizarin Red Stainingmentioning

confidence: 99%

Effects of β-TCP ceramics on intracellular Ca2+ concentration, mineralization of osteoblast and protein structure

Zhang

Dai

et al. 2011

J. Wuhan Univ. Technol.-Mat. Sci. Edit.

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β-TCP, as one of calcium phosphates ceramics, exerts perfect biocompatibility and osteoconductivity, and is clinically used as a bone graft substitute for decades. Consequently, the effects of β-TCP ceramics on intracellular Ca 2+ concentration, mineralization of osteoblast and BSA protein structure were studied. Results showed that β-TCP could increase the intracelluar Ca 2+ concentration and mineralization of osteoblast, indicating that β-TCP ceramics could take part in the organic metabolism and the degradation product had no detrimental effect on osteoblast in vitro. Furthermore, β-TCP ceramics could increase the content of α-helix and β-pleated sheet and change BSA into more ordering structure, those changes might be favorable for the biomineralization after β-TCP ceramics implanted.

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An explanation of the mineralization mechanism in osteoblasts induced by calcium hydroxide

Cited by 44 publications

References 26 publications

Sustained release of calcium hydroxide from poly(dl-lactide-co-glycolide) acid microspheres for apexification

Sustained release of calcium hydroxide from poly(dl-lactide-co-glycolide) acid microspheres for apexification

Effects of various additives on antimicrobial, physical and chemical properties of mineral trioxide aggregate (MTA)

Effects of β-TCP ceramics on intracellular Ca2+ concentration, mineralization of osteoblast and protein structure

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