An experimental vaccine for calf pneumonia caused by Mycoplasma bovis: clinical, cultural, serological and pathological findings

Nicholas, R. A. J.; Ayling, Roger D.; Stipkovits, L.

doi:10.1016/s0264-410x(02)00340-7

Cited by 91 publications

(100 citation statements)

References 16 publications

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“…M. canadense is not able to induce lung infection without other pathogens (25). In our study, M. canis and M. canadense were not found in co-infection with M. bovis, but in co-infection with other mycoplasmas (12,20).…”

Section: Discussioncontrasting

confidence: 45%

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Szacawa

Szymańska-Czerwińska

Niemczuk

et al. 2016

Journal of Veterinary Research

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Introduction: Mycoplasma bovis is one of the main pathogens involved in cattle pneumonia. Other mycoplasmas have also been directly implicated in respiratory diseases in cattle. The prevalence of different Mycoplasma spp. in cattle affected by respiratory diseases and molecular characteristics of M. bovis field strains were evaluated. Material and Methods: In total, 713 nasal swabs from 73 cattle herds were tested. The uvrC gene fragment was amplified by PCR and PCR products were sequenced. PCR/DGGE and RAPD were performed. Results: It was found that 39 (5.5%) samples were positive for M. bovis in the PCR and six field strains had point nucleotide mutations. Additionally, the phylogenetic analysis of 20 M. bovis field strains tested with RAPD showed two distinct groups of M. bovis strains sharing only 3.8% similarity. PCR/DGGE analysis demonstrated the presence of bacteria belonging to the Mollicutes class in 79.1% of DNA isolates. The isolates were identified as: Mycoplasma bovirhinis, M. dispar, M. bovis, M. canis, M. arginini, M. canadense, M. bovoculi, M. alkalescens, and Ureaplasma diversum. Conclusion: Different Mycoplasma spp. strains play a crucial role in inducing respiratory diseases in cattle.

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Section: Discussioncontrasting

confidence: 45%

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Szacawa

Szymańska-Czerwińska

Niemczuk

et al. 2016

Journal of Veterinary Research

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“…This mycoplasma species increases the severity of respiratory disease in calves and can also act as a primary pathogen (Gagea et al, 2006). In Europe, Mycoplasma bovis is believed to be responsible for 25% to 33% of outbreaks of calf pneumonia (Nicholas et al, 2002). In a study of calves in the Netherlands Mycoplasma bovis was isolated from 20% of pneumonic lungs from fattening herds but only in a small number of apparently healthy calves.…”

mentioning

confidence: 99%

Mycoplasma bovis and bacterial pathogens in the bovine respiratory tract

Gabinaitienė¹,

Šiugždaitė²,

Žilinskas³

et al. 2011

Vet. Med. - Czech

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Bovine respiratory disease caused by Mycoplasma bovis is a major health problem of cattle worldwide. It inflicts considerable financial losses on beef herds and is the most common cause of mortality in dairy cattle. Bacteriological examination of 35 nasal cavity samples from calves younger than three months of age identified Mycoplasma bovis in eight (22.9%) samples. These cattle were followed until 17 months of age, and repeated examination of nasal cavity samples before necropsy identified Mycoplasma bovis in four (11.4%) samples. At necropsy and lung samples for bacteriological and histological examination were collected. To identify microorganisms from the Mollicutes class isolated from the nasal cavities of cattle we used the PCR method. Furthermore, Mycoplasma bovis was identified on the grounds of biochemical characteristics and by the disk growth inhibition test. The organism was found in 5.7% of calves younger than three months of age in combination with Pasteurella spp. Mycoplasma bovis in combination with Pasteurella multocida and Mannheimia haemolytica was isolated from 5.7% and 2.9% of cattle at 17 months. However, Pasteurella multocida was common in cattle at 17 months and Mannheimia haemolytica was isolated from both age groups of cattle. Histopathological examination of lung samples revealed broncho-interstitial pneumonia in 14.3% of samples. Mycoplasma bovis was isolated from 60.0% of broncho-insterstitial pneumonia cases. The organism was isolated more frequently from the group of calves rather than from the cattle group (P < 0.05). The most common bacterial agents were Pasteurella multocida and Mannheimia haemolytica.

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“…4). [6,10,16,17]. Shipping fever caused by M. haemolytica type A and P. multocida type A is one of the most important and frequent respiratory diseases of cattle [2,4,8,9].…”

Section: Resultsmentioning

confidence: 99%

“…Mycoplasma bovis is a major causative bacterium of pneumonia and arthritis in calves, and of mastitis and genital infections in adult cows. Although the importance of Mycoplasma bovis in respiratory diseases has been often underestimated, it is responsible for high economic loss in feedlot cattle [16] and is widespread within the bovine population in enzootically infected areas [15].…”

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confidence: 99%

Safety and Efficacy Testing of a Novel Multivalent Bovine Bacterial Respiratory Vaccine Composed of Five Bacterins and Two Immunogens

Cho

Lee

Lim

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Bovine bacterial respiratory diseases have been one of the most serious problems due to their high mortality and economic loss in calves. The vaccinations of bovine bacterial respiratory vaccines have been complex because of no multivalent vaccine. In this study, novel multivalent bovine bacterial respiratory vaccine (BRV) was developed and tested for its safety and efficacy. BRV was composed of two immunogens and five bacterins. These were leukotoxoid and bacterin of Mannheimia haemolytica type A, outer membrane protein and bacterin of Pasteurella multocida type A, and bacterins of Haemophilus somnus, Mycoplasma bovis, and Arcanobacterium pyogenes. ELISA antibody titers to five bacterial antigens in vaccinated guinea pigs increased, compared with those in unvaccinated ones. BRV was safe for calves and pregnant cattle in this study. In calves challenged with M. haemolytica and P. multocida, the average daily weight gain and antibody titers of vaccinated calves increased, and respiratory symptoms (P<0.05) and treatment frequency (P<0.01) of vaccinated calves significantly decreased, compared with those of unvaccinated calves. Interestingly, the antibody titers of M. haemolytica leukotoxoid and Mycoplasma bovis were closely related with the reduction of respiratory symptoms. BRV would be an ecomonical measure for the protection against bovine bacterial respiratory diseases. KEY WORDS: Arcanobacterium pyogenes, Haemophilus somnus, Mannheimia haemolytica leukotoxoid, Mycoplasma bovis, Pasteurella multocida outer membrane protein.

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An experimental vaccine for calf pneumonia caused by Mycoplasma bovis: clinical, cultural, serological and pathological findings

Cited by 91 publications

References 16 publications

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Mycoplasma bovis and bacterial pathogens in the bovine respiratory tract

Safety and Efficacy Testing of a Novel Multivalent Bovine Bacterial Respiratory Vaccine Composed of Five Bacterins and Two Immunogens

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