An experimental study to evaluate the antiosteoporotic effect of Panchatikta Ghrita in a steroid-induced osteoporosis rat model

Abstract

Objective:The study was conducted to develop the glucocorticoid-induced osteoporosis (GIO) model in Sprague-Dawley weanling rats using different doses of methylprednisolone (MP) and evaluate the antiosteoporotic effect of a classical ayurvedic formulation, Panchatikta Ghrita (PG), in this model.Materials and Methods:Institutional Animal Ethics Committee approval was obtained. Development of model was done by subcutaneous injection of 2 doses of MP (14 and 28 mg/kg/week) for 4 weeks in 21-day old weanlings. Following confirmation of the dose of MP that induced osteoporosis, the antiosteoporotic effect of PG was tested in this model in comparison to a known antiosteoporotic agent, alendronate. Both alendronate (2.9 mg/kg/day) and PG (1.35 g/kg/day) were administered orally 2 weeks after MP - 14 mg/kg/week injection and continued for 4 weeks. Serum and urine calcium and inorganic phosphate were analyzed at weekly intervals. Animals were sacrificed after 6 weeks, and femur bones were processed to measure bone hardness and elasticity and for histological studies.Results:Rats treated with MP - 14 mg/kg/week showed optimum osteoporotic effect with no mortality as compared to MP - 28 mg/kg/week; hence, this dose of MP was used further for the efficacy study. Osteoporotic rats treated with PG 1.35 g/kg showed increase in serum calcium and inorganic phosphate levels, whereas urine calcium and phosphate levels were significantly reduced. A significant decrease in a number of osteoclasts, whereas an increase in bone hardness and elasticity was observed as compared to diseased group demonstrating antiosteoporotic effect of PG.Conclusion:PG has an antiosteoporotic effect in GIO rat model.

Does Panchatikta ghrita have anti-osteoporotic effect? Assessment in an experimental model in ovariectomized rats

“…Panchatikta Ghrita (PG), a classical ayurvedic formulation has plant ingredients that, as per Ayurveda, have properties of tikta rasa which maintains the dhatvagni in normal state which in turn keeps all the dhatus in the body in equilibrium. As asthi dhatu (related to bone tissue) becomes stable, bone degeneration decreases [11] , [17] . Thus, this formulation, based on the osteoprotective properties of its plant ingredients, protects against bone degeneration and loss.…”

“…In our previous study, we had studied the protective effect of the same formulation against an experimental model of glucocorticoid induced osteoporosis [11] . The results demonstrated that Panchtikta ghrita protected against worsening of bone degeneration in this model of osteoporosis.…”

“…Decrease in “ asthi ” causes an imbalance of the vata dosha thus weakening the bone structure and thickness. Plants with tikta (bitter) and katu (pungent) properties are said to reverse this imbalance in the dosha [10] , [11] . Panchatikta Ghrita (PG) is a classical Ayurvedic formulation that contains 5 plants with these properties viz.…”

Health benefits of ghee: Review of Ayurveda and modern science perspectives

“…A study on Panchatikta Ghrita (PG) stated its anti-osteoporotic properties in rats. PG administration in osteoporosis induced rats demonstrated better serum calcium and inorganic phosphate levels, reduced urinary calcium and phosphate levels, decreased osteoclasts, and increased bone hardness compared to the diseased group [ 128 ]. Later another study be the same authors show promising effects of PG in protecting against postmenopausal osteoporosis [ 129 ].…”

Age and Sex Features of Bone Mineral Density in Rats

“…Завдяки значному прогресу біології в останні роки існує мож-ливість аналізувати дію багатьох чинників, котрі впливають на метаболізм кісткової тканини, як in vivo, так і in vitro, що призвело до більш активного використання експери-ментального моделювання на тваринах [1][2][3][4][5][6]. Проте існуючі сучасні методи оцінки стану й метаболізму кісткової тканини є інвазивними (гістоморфометричне дослідження, визначен-ня біохімічних маркерів кісткового ремоде-лювання в сироватці крові тощо) і неінвазив-ними (комп'ютерна томографія, двофотонна рентгенівська денситометрія -ДРА), що може обмежувати оцінку результатів дослідження в динаміці експерименту [1,7,8].…”