2006

DOI: 10.1016/j.burns.2005.10.014

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An experimental study on the repair of full skin loss of nude mice with composite graft of epidermal stem cells

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Cited by 8 publications

(6 citation statements)

References 13 publications

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“…The transplantation of the printed skin grafts in athymic nude mouse in the different groups, healed at different rates of time. Previous studies have shown that composite epidermal skin grafts healed *1 week faster than control mice in a comparable experimental setup, 21 corroborating with our results. Clinical data showed that Apligraf may degrade partially or completely after the initial application and in 3-5 days may have an appearance of yellow gelatin.…”

Section: Discussionsupporting

confidence: 82%

“…In addition, human keratinocytes are known to secrete various angiogenic growth factors. 21 Previous work revealed that when human acellular dermis was infiltrated with HaCaT epidermal cells and supplemented with a fibrin gel and antigenic factors, it enhanced neovascularization. [30][31][32] Studies performed by Kremer et al revealed that when Integra Ò was seeded with keratinocytes and implanted on a full-thickness wound, the matrix infiltrated in the wound bed.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In Vivo Assessment of Printed Microvasculature in a Bilayer Skin Graft to Treat Full-Thickness Wounds

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et al. 2015

Tissue Engineering Part A

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Chronic wounds such as diabetic foot ulcers and venous leg ulcers are common problems in people suffering from type 2 diabetes. These can cause pain, and nerve damage, eventually leading to foot or leg amputation. These types of wounds are very difficult to treat and sometimes take months or even years to heal because of many possible complications during the process. Allogeneic skin grafting has been used to improve wound healing, but the majority of grafts do not survive several days after being implanted. We have been studying the behavior of fibroblasts and keratinocytes in engineered capillary-like endothelial networks. A dermo-epidermal graft has been implanted in an athymic nude mouse model to assess the integration with the host tissue as well as the wound healing process. To build these networks into a skin graft, a modified inkjet printer was used, which allowed the deposit of human microvascular endothelial cells. Neonatal human dermal fibroblast cells and neonatal human epidermal keratinocytes were manually mixed in the collagen matrix while endothelial cells printed. A full-thickness wound was created at the top of the back of athymic nude mice and the area was covered by the bilayered graft. Mice of the different groups were followed until completion of the specified experimental time line, at which time the animals were humanely euthanized and tissue samples were collected. Wound contraction improved by up to 10% when compared with the control groups. Histological analysis showed the neoskin having similar appearance to the normal skin. Both layers, dermis and epidermis, were present with thicknesses resembling normal skin. Immunohistochemistry analysis showed favorable results proving survival of the implanted cells, and confocal images showed the human cells' location in the samples that were collocated with the bilayer printed skin graft.

“…The transplantation of the printed skin grafts in athymic nude mouse in the different groups, healed at different rates of time. Previous studies have shown that composite epidermal skin grafts healed *1 week faster than control mice in a comparable experimental setup, 21 corroborating with our results. Clinical data showed that Apligraf may degrade partially or completely after the initial application and in 3-5 days may have an appearance of yellow gelatin.…”

Section: Discussionsupporting

confidence: 82%

“…In addition, human keratinocytes are known to secrete various angiogenic growth factors. 21 Previous work revealed that when human acellular dermis was infiltrated with HaCaT epidermal cells and supplemented with a fibrin gel and antigenic factors, it enhanced neovascularization. [30][31][32] Studies performed by Kremer et al revealed that when Integra Ò was seeded with keratinocytes and implanted on a full-thickness wound, the matrix infiltrated in the wound bed.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Assessment of Printed Microvasculature in a Bilayer Skin Graft to Treat Full-Thickness Wounds

¹

,

²

,

³

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

Chronic wounds such as diabetic foot ulcers and venous leg ulcers are common problems in people suffering from type 2 diabetes. These can cause pain, and nerve damage, eventually leading to foot or leg amputation. These types of wounds are very difficult to treat and sometimes take months or even years to heal because of many possible complications during the process. Allogeneic skin grafting has been used to improve wound healing, but the majority of grafts do not survive several days after being implanted. We have been studying the behavior of fibroblasts and keratinocytes in engineered capillary-like endothelial networks. A dermo-epidermal graft has been implanted in an athymic nude mouse model to assess the integration with the host tissue as well as the wound healing process. To build these networks into a skin graft, a modified inkjet printer was used, which allowed the deposit of human microvascular endothelial cells. Neonatal human dermal fibroblast cells and neonatal human epidermal keratinocytes were manually mixed in the collagen matrix while endothelial cells printed. A full-thickness wound was created at the top of the back of athymic nude mice and the area was covered by the bilayered graft. Mice of the different groups were followed until completion of the specified experimental time line, at which time the animals were humanely euthanized and tissue samples were collected. Wound contraction improved by up to 10% when compared with the control groups. Histological analysis showed the neoskin having similar appearance to the normal skin. Both layers, dermis and epidermis, were present with thicknesses resembling normal skin. Immunohistochemistry analysis showed favorable results proving survival of the implanted cells, and confocal images showed the human cells' location in the samples that were collocated with the bilayer printed skin graft.

“…Skin fibroblast culture was established with tissue explant technique as previously described (Hu et al 2006). Cells were cultured in DMEM (GIBCO, Scotland, UK) supplemented with 10% fetal bovine serum (FBS) (JRH Biosciences, Inc., USA), 100 U/mL penicillin and 100 U/mL streptomycin (GIBCO, UK) and kept in an incubator at 37°C with 5% CO 2 .…”

Section: Cell Culturementioning

confidence: 99%

Compressed collagen gel as the scaffold for skin engineering

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et al. 2010

Biomed Microdevices

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Collagen gel scaffolds can potentially be utilized as cell seeded systems for skin tissue engineering. However, its dramatic contraction after being mixed with cells and its mechanical weakness are the drawbacks for its application to skin engineering. In this study, a compressed collagen gel scaffold was fabricated through the rapid expulsion of liquid from reconstituted gels by the application of 'plastic compression'(PC) technique. Both compressed and uncompressed gels were characterized with their gel contraction rate, morphology, the viability of seeded cells, their mechanical properties and the feasibility as a scaffold for constructing tissue-engineered skin. The results showed that the compression could significantly reduce the contraction of the collagen gel and improve its mechanical property. In addition, seeded dermal fibroblasts survived well in the compressed gel and seeded epidermal cells gradually developed into a stratified epidermal layer, and thus formed tissue engineered skin. This study reveals the potential of using compressed collagen gel as a scaffold for skin engineering.

“…Therefore, a stem cell is characterized by (1) unlimited self-renewal capacity, (2) long-term viability, (3) multi-lineage potential, [12] (4) participation in tissue repair and (5) preservation of somatic homeostasis. [13,14] Stem cells can be harvested from embryonic or adult tissues. A classical stem cell must be capable of asymmetric cell division, i.e.…”

Section: Stem Cellmentioning

confidence: 99%

Role of stem cells in the management of chronic wounds

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2012

Indian J Plast Surg

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Chronic wounds continue to be a major challenge for the medical profession, and plastic surgeons are frequently called in to help in the management of such wounds. Apart from the obvious morbidity to the patient, these problem wounds can be a major drain on the already scarce hospital resources. Sometimes, these chronic wounds can be more taxing than the underlying disease itself. Although many newer methods are available to handle such situations, the role of stem cells in the management of such wounds is an exciting area that needs to be explored further. A review of literature has been done regarding the role of stem cells in the management of chronic wounds. The abnormal pathology in such wounds is discussed and the possible role of stem cells for optimal healing in such cases would be detailed.

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