An Experimental Study on the Features of Peri-Implant Epithelium: Immunohistochemical and Electron-Microscopic Observations

Fujiseki, Masatsugu; Matsuzaka, Kenichi; Yoshinari, Masao; Shimono, Masaki; Inoue, Takashi

doi:10.2209/tdcpublication.44.185

Cited by 25 publications

(37 citation statements)

References 28 publications

(31 reference statements)

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“…Differences between the peri-implant epithelium and the junctional epithelium of natural teeth are well known (6,(9)(10)(11)14). Fujizeki et al reported that the peri-implant epithelium is similar to that seen in the oral mucosa, but that it is structurally different from the functional epithelium (6).…”

Section: Behavior Of the Peri-implant Epithelium And Nerve Endingsmentioning

confidence: 99%

“…Many investigators have studied the differences in functions and morphological features between the peri-implant epithelium and the normal junctional epithelium (6,(9)(10)(11)14). Those studies revealed that the intercellular spaces of peri-implant epithelium are wider, which suggests that subsequent defense reactions might be weaker than those of normal junctional epithelial cells.…”

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confidence: 99%

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Characterization of the peri-implant epithelium in hamster palatine mucosa: Behavior of Merkel cells and nerve endings

et al. 2005

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The purpose of this study was to investigate the relationship between Merkel cells and nerve elements during tissue regeneration after receiving dental implants. Golden hamsters were divided into 3 groups and titanium alloy implants were fixed in their left-side maxilla through the third palatine ruga. Animals were sacrificed at 1, 2, 3, 4, 5, 6, and 7 days after the implantation and tissues were characterized at the immunohistochemical and morphological levels. CK 20 and PGP 9.5 antibodies which react with Merkel cells and nerve fibers were used. Immunohistochemically, no CK20-positive Merkel cells were seen in the peri-implant epithelium throughout the 7 days. However, starting at day 4, PGP 9.5-positive nerve fibers appeared in the connective tissue, and by day 7, nerve fibers had invaded the more superficial layer of the peri-implant epithelium compared to the mucosa removal control group. At the electron microscopic level, the intercellular spaces of the regenerating epithelium in the mucosa removal control group were small. In contrast, intercellular spaces of the peri-implant epithelium tended to be wide and regenerating nerve fibers invaded those intercellular spaces. In both the mucosa removal control group and the implantation group, the basal lamina and connective tissues regenerated completely. However, clear Merkel cells containing neurosecretory granules were not observed. Taken together, our results indicate that Merkel cells in the hamster palatine mucosa do not regenerate in the peri-implant epithelium. However, regenerative nerve fibers seem to play essential roles as part of the defense and sensory systems around the peri-implant epithelium to compensate for the weakened defense mechanism.It is well known that Merkel cells play an important role in tissues as a sensory system for pressure (8,16,28). Previous studies have revealed that Merkel cells are widely distributed in human oral mucosal tissues including the lip, hard palate and mandibular gingiva (1, 13). A fundamental, ultrastructural study of rodent oral mucosa demonstrated that two types of Merkel cells, with dendritic or roundish shapes, are recognized. Most dendritic Merkel cells are free of innervations while the roundish ones are innervated (26). In addition, Merkel cell-neurite complexes are considered to be slowly adapting type I mechanoreceptors in the oral mucosa of the hamster cheek pouch (28,29).The behavior of Merkel cells after denervation has also been elucidated (22), the conclusion being that Merkel cells survive with normal density and appearance in the epidermis after denervation. A study of the behavior of Merkel cells and nerve endings after partial excision of the lip has shown that Merkel cells and nerve fibers are regenerated (25). However, there have been no reports on the behavior of Merkel cells and/or nerve endings in re-

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Section: Behavior Of the Peri-implant Epithelium And Nerve Endingsmentioning

confidence: 99%

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confidence: 99%

Characterization of the peri-implant epithelium in hamster palatine mucosa: Behavior of Merkel cells and nerve endings

et al. 2005

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“…The description of the quality of the BS of the peri-implant mucosa (PIM) has been based on histomorphometric features observed in animal models [1,2,5,6,8,9]. For example, an animal study by Ikeda [6] evaluated qualitatively the BS around implants by tracing the penetration of horseradish peroxidase through the peri-implant tissue interface using light and transmission electron microscopy [6].…”

Section: Introductionmentioning

confidence: 99%

The biological seal of the implant–soft tissue interface evaluated in a tissue-engineered oral mucosal model

Chai

Brook

Palmquist

et al. 2012

J. R. Soc. Interface.

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For dental implants, it is vital that an initial soft tissue seal is achieved as this helps to stabilize and preserve the peri-implant tissues during the restorative stages following placement. The study of the implant -soft tissue interface is usually undertaken in animal models. We have developed an in vitro three-dimensional tissue-engineered oral mucosal model (3D OMM), which lends itself to the study of the implant -soft tissue interface as it has been shown that cells from the three-dimensional OMM attach onto titanium (Ti) surfaces forming a biological seal (BS). This study compares the quality of the BS achieved using the threedimensional OMM for four types of Ti surfaces: polished, machined, sandblasted and anodized (TiUnite). The BS was evaluated quantitatively by permeability and cell attachment tests. Tritiated water (HTO) was used as the tracing agent for the permeability test. At the end of the permeability test, the Ti discs were removed from the three-dimensional OMM and an Alamar Blue assay was used for the measurement of residual cells attached to the Ti discs. The penetration of the HTO through the BS for the four types of Ti surfaces was not significantly different, and there was no significant difference in the viability of residual cells that attached to the Ti surfaces. The BS of the tissue-engineered oral mucosa around the four types of Ti surface topographies was not significantly different.

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“…In addition, they showed a lack of attachment apparatus at the apical portion of the periimplant epithelium. On the other hand, no attachment apparatus was formed between plasma-sprayed ITI implants and the peri-implant epithelium of dogs, and the nature of the epithelium was closer to that of oral mucosal epithelium than to that of junctional epithelium, based on immunohistochemical results (Fujiseki et al, 2003). In their electron micrograph, many microvilli were evident at the periphery of the cells at the implant sites in place of hemidesmosomes and basal lamina after 6 months implantation.…”

Section: Interface Between Dental Implant and Epitheliummentioning

confidence: 97%

Ultrastructure of Dentogingival Border of Normal and Replanted Tooth and Dental Implant

Sawada¹,

Inoué²

2011

Implant Dentistry - The Most Promising Discipline of Dentistry

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An Experimental Study on the Features of Peri-Implant Epithelium: Immunohistochemical and Electron-Microscopic Observations

Cited by 25 publications

References 28 publications

Characterization of the peri-implant epithelium in hamster palatine mucosa: Behavior of Merkel cells and nerve endings

Characterization of the peri-implant epithelium in hamster palatine mucosa: Behavior of Merkel cells and nerve endings

The biological seal of the implant–soft tissue interface evaluated in a tissue-engineered oral mucosal model

Ultrastructure of Dentogingival Border of Normal and Replanted Tooth and Dental Implant

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