Cyclic deformation and the resulted fatigue are experimentally investigated in magnesium single crystal subjected to fully reversed strain-controlled tension-compression in [ ] direction at room temperature. Plastic instability is found near the end of the first detwinning reversal. Twin-twin boundaries and secondary twins are induced by interaction of primary { } twins. Microcracks are developed at primary twin boundaries and twin-twin interaction sites. Final fracture is most likely caused by cleavage-cracking on { }-{ } double twin and shearing fracture on the basal plane.