An experimental investigation of Stichococcus sp. cultivation conditions for optimal co-production of carbohydrates, proteins and lipids following a biorefinery concept

Karapatsia, Anna; Penloglou, Giannis; Chatzidoukas, Christos; Kiparissides, Costas

doi:10.1016/j.biombioe.2016.01.009

Cited by 14 publications

(13 citation statements)

References 26 publications

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“…This relevance of the carbohydrate accumulation in Stichococcus sp. cultures with the increase of dry biomass concentration was additional recorded by a previous study [32].…”

Section: Metabolites Concentrationmentioning

confidence: 94%

“…(identified with the 18S-rDNA gene sequence analysis) was isolated from the Crete coastal area in Southern Greece. Stichococcus is a genus of green algae (Chlorophyta) characterized by a simple morphology, with cell size ranging from 2 to 6 µm, organized in filamentous or unicellular structures [33], with some species (e.g., S. bacillaris) being distinguished for their resistance to temperature, salinity and pH variation, their lipid content, as well as their capacity to remove efficiently heavy metals from wastewater [32,[34][35][36].…”

Section: Microalgae Strain and Pre-culture Conditionsmentioning

confidence: 99%

“…The experiments of Stichococcus sp. cultures implemented in the present study to explore the synergistic role of different light spectrums in regulating either the culture growth rate or cell composition were performed in a lab-scale bench-top photobioreactor (FerMac 320, Electrolab Biotech Limited, UK), with a total capacity of 3 L. The research outcome of a preliminary study [32], published by the same research group, was employed to determine the appropriate PBR operating profile. More specifically, given that the culture aeration rate, CO 2 supply rate, and initial nitrogen concentration in the culture medium were distinguished as the most important operating factors and examined for their contribution to the total variance of biomass and products concentration, their optimal values found in that work were adopted in the present study.…”

Section: Pbr Experimentsmentioning

confidence: 99%

“…In our previous study, the effect of five process variables (i.e., illumination flux, aeration rate, CO 2 supply rate, nitrogen concentration, and salinity) on the performance of Stichococcus sp. cultures was thoroughly investigated [32]. Precisely, employing the Taguchi design experiment method, the biomass production rate and the accumulation capacity of the microalgae population in carbohydrates, proteins, and lipids were correlated with the above key process variables, and the best operating policy that selectively maximizes the component of interest was explored.…”

Section: Introductionmentioning

confidence: 99%

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Illumination Policies for Stichococcus sp. Cultures in an Optimally Operating Lab-Scale PBR toward the Directed Photosynthetic Production of Desired Products

Psachoulia

Chatzidoukas

2021

Sustainability

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The light spectrum effect on the cultivation efficiency of the microalgae strain Stichococcus sp. is explored, as a means of potentially intensifying the biomass productivity and regulating the cellular composition. Stichococcus sp. batch culture experiments, within a 3 L bench-top photobioreactor (PBR), are designed and implemented under different light spectrum profiles (i.e., cool white light (WL), cool white combined with red light (WRL), and cool white combined with blue light, (WBL)). The obtained results indicate that the studied strain is capable of adapting its metabolite profile to the light field to which it is exposed. The highest biomass concentration (3.5 g/L), combined with intense carbohydrate accumulation activity, resulting in a respective final concentration of 1.15 g/L was achieved within 17 days using exclusively cool white light of increasing intensity. The addition of blue light emitting diodes (LED) light, combined with appropriately selected culture conditions, contributed significantly to the massive synthesis and accumulation of lipids, resulting in a concentration of 1.43 g/L and a respective content of 46.13% w/w, with a distinct impact on biomass, carbohydrates and proteins productivity. Finally, a beneficial contribution of red LED light to the protein synthesis is recognized and this can be conditionally amplified provided nitrogen sufficiency in the culture medium.

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“…This relevance of the carbohydrate accumulation in Stichococcus sp. cultures with the increase of dry biomass concentration was additional recorded by a previous study [32].…”

Section: Metabolites Concentrationmentioning

confidence: 94%

Section: Microalgae Strain and Pre-culture Conditionsmentioning

confidence: 99%

Section: Pbr Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Illumination Policies for Stichococcus sp. Cultures in an Optimally Operating Lab-Scale PBR toward the Directed Photosynthetic Production of Desired Products

Psachoulia

Chatzidoukas

2021

Sustainability

Self Cite

View full text Add to dashboard Cite

show abstract

“…(identified with the 18S-rDNA gene sequence analysis) was isolated from the Crete coastal area in Southern Greece. Stichococcus is a genus of green algae (Chlorophyta) characterized by a simple morphology, with cell size ranging from 2 to 6 μm, organized in filamentous or unicellular structures [33], with some species (e.g., S. bacillaris) being distinguished for their resistance to temperature, salinity and pH variation, their lipid content, as well as their capacity to remove efficiently heavy metals from wastewater [32,[34][35][36].…”

Section: Microalgae Strain and Pre-culture Conditionsmentioning

confidence: 99%