An experimental design approach using response surface techniques to obtain optimal liquid chromatography and mass spectrometry conditions to determine the alkaloids in Meconopsi species

Zhou, Yan; Song, Jing‐Zheng; Choi, Franky Fung-Kei; Wu, Haifeng; Qiao, Chun‐Feng; Ding, Li‐Sheng; Gesang, Suo-Lang; Xu, Hong‐Xi

doi:10.1016/j.chroma.2009.08.058

Cited by 70 publications

(37 citation statements)

References 35 publications

(43 reference statements)

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“…Meconopsis (Papaveraceae) comprises about 43-49 species with the majority of these restricted to the Himalaya and only one species, M. cambrica, being endemic to Europe (Debnath and Nayar, 1986;Zhou et al, 2009 (Luo et al, 1984;Zhou et al, 2009;Yue et al, 2010). Out of 13 Meconopsis species reported from Bhutan (Grierson and Long, 1984), seven of them (M. horridula, M. paniculata, M. napaulensis, M. superba, M. primulina, M. discigera and M. simplicifolia) were said to have medicinal properties.…”

Section: Introductionmentioning

confidence: 99%

A new protoberberine alkaloid from Meconopsis simplicifolia (D. Don) Walpers with potent antimalarial activity against a multidrug resistant Plasmodium falciparum strain

Wangchuk

Keller

Pyne

et al. 2013

Journal of Ethnopharmacology

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A new protoberberine alkaloid from Meconopsis simplicifolia (D. Don) Walpers with potent antimalarial activity against a multidrug resistant Plasmodium falciparum strain AbstractEthnopharmacological relevance The aerial components of Meconopsis simplicifolia (D. Don) Walpers are indicated in Bhutanese traditional medicine for treating malaria, coughs and colds, and the infections of the liver, lung and blood. This study is to validate the ethnopharmacological uses of this plant and also identify potent antimalarial drug leads through bioassays of its crude extracts and phytochemical constituents.Materials and methods Meconopsis simplicifolia (D. Don) Walpers was collected from Bhutan and its crude MeOH extract was subjected to acid-base fractionation. Through repeated extractions, separations and spectroscopic analysis, the alkaloids obtained were identified and tested for their antimalarial and cytotoxicity activities.Results Phytochemical studies resulted in the isolation of one new protoberberine type alkaloid which we named as simplicifolianine and five known alkaloids: protopine, norsanguinarine, dihydrosanguinarine, 6-methoxydihydrosanguinarine and oxysanguinarine. Among the five of the alkaloids tested, simplicifolianine showed the most potent antiplasmodial activities against the Plasmodium falciparum strains, TM4/8.2 (chloroquine-antifolate sensitive strain) and K1CB1 (multidrug resistant strain) with IC50 values of 0.78 μg/ mL and 1.29 μg/mL, respectively. The compounds tested did not show any significant cytotoxicity activities against human oral carcinoma KB cells and normal Vero cells of African kidney epithelial cells.Conclusions This study validated the traditional uses of the plant for the treatment of malaria and identified a new alkaloid, simplicifolianine as a potential antimalarial drug lead.

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Section: Introductionmentioning

confidence: 99%

A new protoberberine alkaloid from Meconopsis simplicifolia (D. Don) Walpers with potent antimalarial activity against a multidrug resistant Plasmodium falciparum strain

Wangchuk

Keller

Pyne

et al. 2013

Journal of Ethnopharmacology

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“…Zhou et al used response surface methodology (a statistic approach) for optimization of UHPLC-QTOF conditions to analyze the main alkaloids of Meconopsis species [175]. The optimization was carried out with Box-Behnken designs (BBD) and the multicriteria response variables were described using global Derringer's desirability.…”

Section: Alkaloidsmentioning

confidence: 99%

“…Zhou et al quantified 12 diterpenoids in 25 samples of crude extracts from 12 Salvia species using UHPLC-QTOF [180]. The whole separation was accomplished Reproduced with permission from [175]. Reproduced with permission from [175].…”

Section: Terpenoidsmentioning

confidence: 99%

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Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Guo

Chen

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

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158

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“…26 The Box-Behnken design (BBD) is one popular form of RSM, and as it appears to be more effective than many of the other forms, it is now acknowledged as one of the best statistical and analytical models for RSM. 27,28 In the present study, PHY-loaded PLA (PHYP) nanospheres were prepared by double emulsion solvent evaporation, and the preparation was evaluated by RSM to identify the optimum conditions. The variables (concentration of poloxamer 199 [F68] [w/v], concentration of PLA [mg/mL], and ratio of PLA to drug [w/w]) in the PHYP nanospheres preparation process were investigated and optimized using BBD (three factors and three levels).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

Zheng

Luo

et al. 2016

IJN

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This study aimed to optimize the preparation conditions of pachyman (PHY)-loaded poly( d,l -lactic acid) (PLA) (PHYP) nanospheres by response surface methodology, explore their characteristics, and assess their effects on splenic lymphocytes. Double emulsion solvent evaporation was used to synthesize PHYP nanospheres, and the optimal preparation conditions were identified as a concentration of poloxamer 188 (F68) (w/v) of 0.33%, a concentration of PLA of 30 mg/mL, and a ratio of PLA to drug (w/w) of 10.25:1 required to reach the highest encapsulation efficiency, which was calculated to be 59.10%. PHYP had a spherical shape with a smooth surface and uniform size and an evident effect of sustained release and relative stability. Splenic lymphocytes are crucial and multifunctional cells in the immune system, and their immunological properties could be enhanced significantly by PHYP treatment. This study confirmed that PHY encapsulated in PLA nanospheres had comparatively steady properties and exerted obvious immune enhancement.

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An experimental design approach using response surface techniques to obtain optimal liquid chromatography and mass spectrometry conditions to determine the alkaloids in Meconopsi species

Cited by 70 publications

References 35 publications

A new protoberberine alkaloid from Meconopsis simplicifolia (D. Don) Walpers with potent antimalarial activity against a multidrug resistant Plasmodium falciparum strain

A new protoberberine alkaloid from Meconopsis simplicifolia (D. Don) Walpers with potent antimalarial activity against a multidrug resistant Plasmodium falciparum strain

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

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