An expedition in the jungle of pluripotent stem cells of non-human primates

Anwised, Preeyanan; Moorawong, Ratree; Samruan, Worawalan; Somredngan, Sirilak; Srisutush, Jittanun; Laowtammathron, Chuti; Aksoy, Irene; Parnpai, Rangsun; Savatier, Pierre

doi:10.1016/j.stemcr.2023.09.013

Cited by 2 publications

(1 citation statement)

References 119 publications

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“…Nevertheless, several reports over the last few years have expanded the panel of NHP-iPSCs generated under feeder-free and transgene-free conditions suitable for preclinical testing [ 8 , 9 , 10 ]. This expanding panel of species includes the baboon, rhesus macaque, cynomolgus macaque, and marmoset [ 7 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions

Tereshchenko,

Esiyok,

Garea-Rodríguez

et al. 2024

Cells

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Non-human primates (NHPs) are pivotal animal models for translating novel cell replacement therapies into clinical applications, including validating the safety and efficacy of induced pluripotent stem cell (iPSC)-derived products. Preclinical development and the testing of cell-based therapies ideally comprise xenogeneic (human stem cells into NHPs) and allogenic (NHP stem cells into NHPs) transplantation studies. For the allogeneic approach, it is necessary to generate NHP-iPSCs with generally equivalent quality to the human counterparts that will be used later on in patients. Here, we report the generation and characterization of transgene- and feeder-free cynomolgus monkey (Macaca fascicularis) iPSCs (Cyno-iPSCs). These novel cell lines have been generated according to a previously developed protocol for the generation of rhesus macaque, baboon, and human iPSC lines. Beyond their generation, we demonstrate the potential of the novel Cyno-iPSCs to differentiate into two clinically relevant cell types, i.e., cardiomyocytes and neurons. Overall, we provide a resource of novel iPSCs from the most frequently used NHP species in the regulatory testing of biologics and classical pharmaceutics to expand our panel of iPSC lines from NHP species with high relevance in preclinical testing and translational research.

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