An expanded genetic linkage map ofPrunusbased on an interspecific cross between almond and peach

Abstract

The genetic linkage map of Prunus constructed earlier and based on an interspecific F2 population resulting from a cross between almond (Prunus dulcis D.A. Webb) and peach (Prunus persica L. Batsch) was extended to include 8 isozyme loci, 102 peach mesocarp cDNAs, 11 plum genomic clones, 19 almond genomic clones, 7 resistance gene analogs (RGAs), 1 RGA-related sequence marker, 4 morphological trait loci, 3 genes with known function, 4 simple sequence repeat (SSR) loci, 1 RAPD, and 1 cleaved amplified polymorphic sequence (CAP) marker. This map contains 161 markers placed in eight linkage groups that correspond to the basic chromosome number of the genus (x = n = 8) with a map distance of 1144 centimorgans (cM) and an average marker density of 6.8 cM. Four more trait loci (Y, Pcp, D, and SK) and one isozyme locus (Mdh1) were assigned to linkage groups based on known associations with linked markers. The linkage group identification numbers correspond to those for maps published by the Arús group in Spain and the Dirlewanger group in France. Forty-five percent of the loci showed segregation distortion most likely owing to the interspecific nature of the cross and mating system differences between almond (obligate outcrosser) and peach (selfer). The Cat1 locus, known to be linked to the D locus controlling fruit acidity, was mapped to linkage group 5. A gene or genes controlling polycarpel fruit development was placed on linkage group 3, and control of senesced leaf color (in late fall season) (LFCLR) was mapped to linkage group 1 at a putative location similar to where the Y locus has also been placed.

Multiple Models for Rosaceae Genomics

“…Partial maps for sour cherry (Wang et al, 1998), sweet cherry (Dirlewanger et al, 2004b), and interspecific crosses between peach and other species, e.g. almond (Joobeur et al, 1998;Bliss et al, 2002;Aranzana et al, 2003;Dirlewanger et al, 2004b), P. davidiana (Foulongne et al, 2003), P. cerasifera (Dirlewanger et al, 2004a), and P. ferganensis , have been developed. Maps for apple (Hemmat et al, 1994;Maliepaard et al, 1998;Liebhard et al, 2003aLiebhard et al, , 2003bLiebhard et al, , 2003cKenis and Keulemans, 2005;Naik et al, 2006;SilfverbergDilworth et al, 2006), pear (Yamamoto et al, 2004), raspberry (Graham et al, 2004), diploid strawberry (Davis and Yu, 1997;Sargent et al, 2004Sargent et al, , 2006Cipriani et al, 2006), cultivated octoploid strawberry (LerceteauKohler et al, 2003;Weebadde et al, 2008), and rose (Debener and Mattiesch, 1999;Debener et al, 2001;Rajapakse et al, 2001;Yan et al, 2005) are also available.…”

Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model

“…Using the information found on the T×E map, other maps were constructed and genetic analyses were performed that were associated with agronomic traits in almond populations (Joobeur et al, 2000;Ballester et al, 2001), almond × peach (Jáuregui et al, 2001;Bliss et al, 2002), apricot (Vilanova et al, 2003;, and peach (Dettori et al, 2001;Yamamoto et al, 2001;Etienne et al, 2002;Foulongne et al, 2003). However, the use of these maps is limited in Prunus because most were constructed with RFLPs that require complex and laborious laboratory procedures.…”

“…The T×E map was thus expanded to 562 markers covering 519 cM with a mean density of 0.92 cM per marker; 87% of the loci corresponded to known DNA sequences and 37% of these were associated with a putative protein. Comparing the positions of the anchor markers of the T×E map (RFLPs, SSRs, and isoenzymes) with those of maps constructed with other Prunus populations (Viruel et al, 1995;Joobeur et al, 2000;Ballester et al, 2001;Jáuregui et al, 2001;Dettori et al, 2001;Yamamoto et al, 2001;Etienne et al, 2002;Bliss et al, 2002;Vilanova et al, 2003;Foulongne et al, 2003;Lambert et al, 2004), it was found that the genomes of the diploid (2n = 16) species peach, almond, apricot, cherry, P. davidiana (Carrière) N.E. Br., P. cerasifera, and P. ferganensis (Kostov & Rjabov) Kovalev & Kostov are essentially co-linear; it was therefore concluded that the genus Prunus can be treated as a single genetic unit.…”

Tri-/Hexanucleotide Microsatellite Markers in Peach Derived from Enriched Genomic Libraries and Their Application in Rosaceae

“…Genetic linkage maps have been constructed for peach, almond, peach × almond and cherry (Joobeur et al 2000, Bliss et al 2002, Dirlewanger et al 2004, Lambert et al 2004. One of these, a map constructed with the F 2 of a cross between 'Texas' almond and 'Earlygold' peach, has been adopted by the stone fruit community as a reference of the genus (Dirlewanger et al 2004).…”