An exon-biased biophysical approach and NMR spectroscopy define the secondary structure of a conserved helical element within the HOTAIR long non-coding RNA

Abzhanova, Ainur; Hirschi, Alexander; Reiter, Nicholas J.

doi:10.1016/j.jsb.2021.107728

Cited by 3 publications

(2 citation statements)

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“…Instead, HOTAIR is highly dynamic, despite maintaining a discernible, characteristic shape [19 ▪ ]. Such dynamics do not prevent HOTAIR from forming stable, well organized substructures, such as stem H10, which could recently be visualized at high resolution by nuclear magnetic resonance (NMR) [20 ▪ ], confirming previous structural models based on SHAPE and DMS probing [21]. Both these cancer-related lncRNAs differ even more remarkably from Braveheart, a mouse lncRNA involved in heart differentiation, for which a SAXS-based approach captured the interaction between its functional AGIL right-hand turn motif and its partner protein CNBP [13 ▪ ].…”

Section: Text Of Reviewmentioning

confidence: 99%

The multiple molecular dimensions of long noncoding RNAs that regulate gene expression and tumorigenesis

Marcia

2022

Current Opinion in Oncology

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Purpose of reviewLncRNAs are emerging as key regulators of gene expression and they ensure homeostasis during cell differentiation and development, replication, and adaptation to the environment. Because of their key central role in regulating the biology of living cells, it is crucial to characterize how lncRNAs function at the genetic, transcriptomic, and mechanistic level. Recent findingsThe low endogenous abundance and high molecular complexity of lncRNAs pose unique challenges for their characterization but new methodological advances in biochemistry, biophysics and cell biology have recently made it possible to characterize an increasing number of these transcripts, including oncogenic and tumor suppressor lncRNAs. These recent studies specifically address important issues that had remained controversial, such as the selectivity of lncRNA mechanisms of action, the functional importance of lncRNA sequences, secondary and tertiary structures, and the specificity of lncRNA interactions with proteins.

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Section: Text Of Reviewmentioning

confidence: 99%

The multiple molecular dimensions of long noncoding RNAs that regulate gene expression and tumorigenesis

Marcia

2022

Current Opinion in Oncology

View full text Add to dashboard Cite

show abstract

“…Interestingly, NMR has shown that one of the domains computationally predicted to form a stem structure does not form under in vitro conditions. Abzhanova et al [17] estimated the conformational heterogeneity of all exonic regions of HOTAIR lncRNA with native gels at different MgCl 2 concentrations. Exon 4, which displayed no variability under any of the salt conditions, was selected for structural characterization by both NMR and SHAPE.…”

Section: Introductionmentioning

confidence: 99%