An Exceptionally Selective Lead(<scp>II</scp>)‐Regulatory Protein from <i>Ralstonia Metallidurans</i>: Development of a Fluorescent Lead(<scp>II</scp>) Probe

Chen, Peng; Greenberg, Bill; Taghavi, Safiyh; Romano, Christine A.; Lelie, Daniël van der; He, Chuan

doi:10.1002/anie.200462443

Cited by 157 publications

(93 citation statements)

References 44 publications

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“…[1][2][3] It is well known that heavymetal ions released into the environment affect ecological life because they tend to accumulate in living organisms and exhibit a high toxicity when adsorbed into the body. [4,5] The removal of heavy-metal ions has been traditionally carried out by several techniques such as membrane processes (for example, dialysis, electrodialysis, reverse-osmosis), neutralization-precipitation and ion exchange, but usually with limited success.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Effective Adsorption of Lead Ions on Fine Poly(phenylenediamine) Microparticles

Huang

Peng

2006

Chemistry A European J

195

123

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Fine microparticles of poly(p-phenylenediamine) (PpPD) and poly(m-phenylenediamine) (PmPD) were directly synthesized by a facile oxidative precipitation polymerization and their strong ability to adsorb lead ions from aqueous solution was examined. It was found that the degree of adsorption of the lead ions depends on the pH, concentration, and temperature of the lead ion solution, as well as the contact time and microparticle dose. The adsorption data fit the Langmuir isotherm and the process obeyed pseudo-second-order kinetics. According to the Langmuir equation, the maximum adsorption capacities of lead ions onto PpPD and PmPD microparticles at 30 degrees C are 253.2 and 242.7 mg g(-1), respectively. The highest adsorptivity of lead ions is up to 99.8 %. The adsorption is very rapid with a loading half-time of only 2 min as well as initial adsorption rates of 95.24 and 83.06 mg g(-1) min(-1) on PpPD and PmPD particles, respectively. A series of batch experiment results showed that the PpPD microparticles possess an even stronger capability to adsorb lead ions than the PmPD microparticles, but the PmPD microparticles, with a more-quinoid-like structure, show a stronger dependence of lead-ion adsorption on the pH and temperature of the lead-ion solution. A possible adsorption mechanism through complexation between Pb(2+) ions and ==N-- groups on the macromolecular chains has been proposed. The powerful lead-ion adsorption on the microparticles makes them promising adsorbents for wastewater cleanup.

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Section: Introductionmentioning

confidence: 99%

Rapid and Effective Adsorption of Lead Ions on Fine Poly(phenylenediamine) Microparticles

Huang

Peng

2006

Chemistry A European J

195

123

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“…This challenge is especially imposing for metal sensing (1)(2)(3)(4)(5), because many metal ions are very similar and sometimes even identical in charge, ionic radius, and other properties, making them difficult to detect at ultra-low concentrations with no interference by other metal ions. Meeting such a challenge is critical in advancing a number of fields including chemistry, biology, environmental engineering, and medicine.…”

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confidence: 99%

A catalytic beacon sensor for uranium with parts-per-trillion sensitivity and millionfold selectivity

Liu

Brown

Meng

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

481

476

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Here, we report a catalytic beacon sensor for uranyl (UO 2 2؉ ) based on an in vitro-selected UO 2 2؉ -specific DNAzyme. The sensor consists of a DNA enzyme strand with a 3 quencher and a DNA substrate with a ribonucleotide adenosine (rA) in the middle and a fluorophore and a quencher at the 5 and 3 ends, respectively. The presence of UO 2 2؉ causes catalytic cleavage of the DNA substrate strand at the rA position and release of the fluorophore and thus dramatic increase of fluorescence intensity. The sensor has a detection limit of 11 parts per trillion (45 pM), a dynamic range up to 400 nM, and selectivity of >1-million-fold over other metal ions. The most interfering metal ion, Th(IV), interacts with the fluorescein fluorophore, causing slightly enhanced fluorescence intensity, with an apparent dissociation constant of Ϸ230 M. This sensor rivals the most sensitive analytical instruments for uranium detection, and its application in detecting uranium in contaminated soil samples is also demonstrated. This work shows that simple, cost-effective, and portable metal sensors can be obtained with similar sensitivity and selectivity as much more expensive and sophisticated analytical instruments. Such a sensor will play an important role in environmental remediation of radionuclides such as uranium.DNA ͉ DNAzyme ͉ fluorescence ͉ deoxyribozyme ͉ catalytic DNA

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“…The drawbacks of them are time-consuming, expensive, and/or required sophisticated equipments. Compared with them, chemical probe is a sensitive, simple and fast method for quantifying Pb 2+ and has attracted much attention [28][29][30][31]. Table 1 showed the comparison of different probes for determination of Pb 2+ .…”

Section: Resultsmentioning

confidence: 98%

Nanoclusters prepared from a silver/gold alloy as a fluorescent probe for selective and sensitive determination of lead(II)

et al. 2014

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We report on the preparation of water-soluble and fluorescent silver/gold alloy nanoclusters (Ag/Au NCs) by a galvanic replacement reaction. The alloy NCs have an average diameter of 1.5 nm and are shown to be viable fluorescent probes for the determination of lead(II) due to aggregation-induced quenching of fluorescence. The alloy NCs are characterized in terms of photoluminescence spectrum, photoluminescence excitation spectrum, transmission electron microscopy and Xray photoelectron spectroscopy which confirm the presence of alloy NCs. Bovine serum albumin, a well known stabilizing agent, has a high-affinity site for Pb 2+ ion, and the resulting complex acts as a quencher of fluorescence. This finding has led to a method for selective determination of Pb 2+ with a limit of detection of about 2 nM.

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An Exceptionally Selective Lead(II)‐Regulatory Protein from Ralstonia Metallidurans: Development of a Fluorescent Lead(II) Probe

Cited by 157 publications

References 44 publications

Rapid and Effective Adsorption of Lead Ions on Fine Poly(phenylenediamine) Microparticles

Rapid and Effective Adsorption of Lead Ions on Fine Poly(phenylenediamine) Microparticles

A catalytic beacon sensor for uranium with parts-per-trillion sensitivity and millionfold selectivity

Nanoclusters prepared from a silver/gold alloy as a fluorescent probe for selective and sensitive determination of lead(II)

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