An examination of the hexane extract of flue-cured tobacco involving gel permeation chromatography

Cook, Clarence; Twine, Margaret; Tallent, Clyde; Harper, Ingeborg T.; Heunisch, George W.; Lewis, Judith B.; Wall, Monroe E.

doi:10.1016/s0031-9422(00)86350-5

Cited by 16 publications

(7 citation statements)

References 17 publications

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“…The total sterol content per g tobacco was 1606 µg of which 165 µg or 10.3% was cholesterol and the smoke condensate per g tobacco smoked contained 21 µg of cholesterol. Even though the presence of cholesterol in tobacco has been reported (Cook et al, 1967(Cook et al, , 1969Davis etal., 1968;Keller et al, 1969;Richardson et al, 1968;Stedman, 1968), its presence in tobacco smoke condensate had not previously been cited.…”

Section: Resultsmentioning

confidence: 99%

Cholesterol in Cigarette Smoke Condensate

Grünwald¹,

Dl²,

Lp³

1971

J. Agric. Food Chem.

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Section: Resultsmentioning

confidence: 99%

Cholesterol in Cigarette Smoke Condensate

Grünwald¹,

Dl²,

Lp³

1971

J. Agric. Food Chem.

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“…< Subsequently, identified in tobacco were several phytosterols [stigmasterol (834), sitosterol (835), campesterol (836), ergosterol (835)] and cholesterol (837,838) and their longchained aliphatic esters (839). All were also identified in tobacco smoke components (838,840,841).…”

Section: Controversies Over the Roffo Investigationsmentioning

confidence: 99%

The Chemical Components Identified in Tobacco and Tobacco Smoke Prior to 1954: A Chronology of Classical Chemistry

Rodgman¹,

Perfetti²

2009

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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Because of the excellent fractionation and identification technologies developed during the early-1950s, the compositions of tobacco and tobacco smoke, both classified as highly complex mixtures, have been defined more completely than the composition of any other highly complex commercial product such as coffee. By year-end 1953, the many years of research by scientists using classical chemical techniques to define the composition of tobacco and its smoke provided meaningful information on the nature of over 300 tobacco components and fewer than 100 tobacco smoke components. Those involved in the pre-1954 research not only provided the cornerstone of our knowledge of the two compositions but also deserve the gratitude of their successors for the early information generated on tobacco and its smoke. This article is our tribute to those researchers who generated much meaningful knowledge on the composition of tobacco and tobacco smoke prior to 1954 despite the now known fractionation and analytical limitations of the so-called classical chemical techniques. It also notes the similarity of some of the early and more recent research results obtained on the chemical and biological properties of smoke condensate and several of its components from tobacco with those obtained by Roffo in the 1930s on a destructive distillate of tobacco.

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“…Silica gel chromatography had been investigated for separating the hexane extract of flue-cured tobacco [27]. Pigments and some impurities in crude solanesol were eluted before solanesol using solvent of petroleum ether-acetone (90:10, v/v).…”

Section: Purity and Yield Of Solanesol By Silica Column Chromatographymentioning

confidence: 99%

Extraction and purification of solanesol from tobacco(I). Extraction and silica gel column chromatography separation of solanesol

Tang

Zhang

Chen

et al. 2007

Separation and Purification Technology

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An examination of the hexane extract of flue-cured tobacco involving gel permeation chromatography

Cited by 16 publications

References 17 publications

Cholesterol in Cigarette Smoke Condensate

Cholesterol in Cigarette Smoke Condensate

The Chemical Components Identified in Tobacco and Tobacco Smoke Prior to 1954: A Chronology of Classical Chemistry

Extraction and purification of solanesol from tobacco(I). Extraction and silica gel column chromatography separation of solanesol

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