An examination of the factors affecting intestinal lymphatic transport of dietary lipids

Resistant Maltodextrin Decreases Micellar Solubility of Lipids and Diffusion of Bile Salt Micelles and Suppresses Incorporation of Micellar Fatty Acids into Caco-2 Cells

“…Glycerin was purchased from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan). [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]Oleic acid was purchased from Amersham Pharmacia Biotech (Tokyo, Japan). High oleic safflower oil is a product of Nisshin Oillio (Tokyo, Japan).…”

“…During semiconfluence, the cells were reseeded on a presoaked membrane filter coated with collagen (Transwell-COL, Corning Costar) and grown to full confluence, according to the method in our previous study (13). A bile salt micellar solution containing 0.5 mm MO, 1 mm oleic acid, [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C] oleic acid, 0.6 mm PC, 0.1 mm cholesterol, 6.6 mm sodium taurocholate, alone (control) or plus 40 mg/ mL RMD was prepared in DMEM by sonication. After filtration through a 0.22-mm filter, 1.5 mL of the micellar solution supplemented with 10% FBS was added to the apical side of differentiated Caco-2 cells and 2.5 mL DMEM was added to the basolateral side.…”

“…8, the incorporation of [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C] oleic acid into Caco-2 cells was significantly smaller in the RMD-containing micelles than that in the control micelles after both 30-and 60-min incubation. Less than 0.65% of the radioactivity added as micellar solutions was secreted to the basolateral side, and no difference was observed between the control and RMD-containing micelles.…”

“…When the diffusion rate of bile salt micelles through a filter membrane was investigated in vitro, bile salt micelles containing RMD diffused more slowly than those without RMD. Incorporation of [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C] oleic acid into Caco-2 cells from the RMD-containing bile salt micelles was significantly smaller than that from the control micelles (without RMD). These results show that RMD suppresses intestinal absorption of lipids by decreasing their micellar solubility and the diffusion rate of bile salt micelles.…”

“…It is known that hydrolyzed products of lipids are incorporated into bile acid micelles in the small intestine, and the micelles diffuse to the intestinal epithelial cell surface through the unstirred water layer (7). Thus, the solubilization of these components in bile salt micelles and the rate of diffusion of bile salt micelles are crucial factors for lipid absorption and digestion.…”