An Examination of Adrenergic Axons Around Pancreatic Arterioles of the Cat for the Presence of Acetylcholinesterase by High Resolution Autoradiographic and Histochemical Methods

Acetylcholine in adrenergic terminals of the cat iris

“…These observations have been confirmed and extended to other organs (heart, vas deferens, Thoenen, Tranzer, Hurlimann & Haefely, 1966;Tranzer & Thoenen, 1967;Graham, Lever & Spriggs, 1968;HMkfelt, 1967;Hokfelt & Jonsson, 1968;Ochi, Konishi, Yoshikawa & Sano, 1968;Ehinger, Falck & Sporrong, 1970;Geltzer, 1969;Roth & Richardson, 1969). This morphological feature suggests that an interaction between peripheral vegetative nerve terminals could explain the findings of Burn & Rand and their collaborators just as could a cholinergic link in the adrenergic neuro-effector mechanism.…”

“…With the electron microscope, it has also recently become possible to identify adrenergic and non-adrenergic terminals. In the rat heart and iris, cat and rabbit iris, cat and guinea-pig vas deferens, and guinea-pig pancreas it has been confirmed that adrenergic and nonadrenergic nerve terminals run in the same Schwann cell strand (Thoenen et al 1966;Tranzer & Thoenen, 1967;H6kfelt, 1967;Graham et at. 1968;562 ACh IN ADRENERGIC NERVE FIBRES Hokfelt & Jonsson, 1968;Ochi et al 1968;Geltzer, 1969;Roth & Richardson, 1969;Ehinger et al 1970).…”

In pursuit of the α‐adrenoceptor: a fine‐structural and electron autoradiographic study using 3H‐phenoxybenzamine and smooth muscle from the cat and guinea‐pig

“…Examination of electron micrographs of the synaptic region of the adrenergic nerves in close apposition to smooth muscle in several organs from several species (Lever, Graham, Irvine & Chick, 1965Esterhuizen, Graham, Lever & Spriggs, 1967Graham, Lever & Spriggs, 1968a; did not reveal any morphological subsynaptic specialization which could with confidence be attributed to an organized receptor area comparable, for example, to that of the motor end plate region of skeletal muscle. However, it was observed that the plasma membrane on the adventitial aspect of arteriolar smooth muscle cells had significantly more pinocytotic vesicles than did the endothelial aspects of the muscle membrane (Lever et al, 1965).…”

An Inhibitory Effect of Atropine on Responses of the Vas Deferens of the Mouse to Field Stimulation

“…Previous pharmacological studies of the vas deferens have been confined largely to organs obtained from guinea-pigs or rats and the innervation of these structures is considered to be adrenergic in nature (Hukovic, 1961;Birmingham & Wilson, 1963;Graham, Al Katib & Spriggs, 1968;Swedin, 1971;Furness, 1974). The existence of a physiologically important cholinergic component in the innervation was largely accounted for by the discovery of ganglionic relays in what had hitherto been considered as classical post-ganglionic sympathetic nerves (Sjostrand, 1962;Graham et al, 1968).…”

“…The existence of a physiologically important cholinergic component in the innervation was largely accounted for by the discovery of ganglionic relays in what had hitherto been considered as classical post-ganglionic sympathetic nerves (Sjostrand, 1962;Graham et al, 1968). Postganglionic neurotransmission is not sensitive to hemicholinium (Bentley & Sabine, 1963) and is resistant to blockade by atropine (Bentley & Sabine, 1963;Birmingham & Wilson, 1963;Graham et al, 1968).…”