An ex vivo model of human skin photoaging induced by UVA radiation compatible with summer exposure in Brazil

Saguie, Bianca Oliveira; Martins, Rayssa Lopes; Fonseca, Adenilson de Souza da; Romana‐Souza, Bruna; Monte‐Alto‐Costa, Andréa

doi:10.1016/j.jphotobiol.2021.112255

Cited by 15 publications

(3 citation statements)

References 35 publications

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“…The UV irradiation treatment of suspensions was achieved for 20 min using a UV lamp (with wavelengths between 300 and 400 nm, a power flux density of 50 mW/cm²). Noteworthy, ex vivo studies were carried on to mimic the skin exposure to 100 day UV irradiation at 16 J/cm² [65]. This amount was harmful, however, in the present study the exposure was at 6.10 -5 J/cm² only.…”

Section: Study Of Cf Release From Photoresponsive Liposomes After Irr...mentioning

confidence: 87%

Photoresponsive liposomes and LipoParticles by incorporating a photosensitizer agent in their lipid membrane

Régagnon

Bugnicourt-Moreira

Ravaz

et al. 2023

Journal of Photochemistry and Photobiology A: Chemistry

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Section: Study Of Cf Release From Photoresponsive Liposomes After Irr...mentioning

confidence: 87%

Photoresponsive liposomes and LipoParticles by incorporating a photosensitizer agent in their lipid membrane

Régagnon

Bugnicourt-Moreira

Ravaz

et al. 2023

Journal of Photochemistry and Photobiology A: Chemistry

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“…However, skin is a multilayered, complex organ that consists of many cells, including fibroblasts, melanocytes, etc. Under excessive ROS, these cells promote more complex physiological phenomena, such as collagen degradation, pigmentation, and wrinkle generation, eventually leading to skin aging and melasma [ 33 , 34 , 35 ]. Given the above reasons, the mixture of hemin and tyrosine may also have a protective effect against UVA-induced damage to other skin cells and provide more comprehensive skin protection.…”

Section: Discussionmentioning

confidence: 99%

Hemin with Peroxidase Activity Can Inhibit the Oxidative Damage Induced by Ultraviolet A

Hui

Yang

Fang

et al. 2022

CIMB

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Excessive reactive oxygen species (ROS), a highly reactive substance that contains oxygen, induced by ultraviolet A (UVA) cause oxidative damage to skin. We confirmed that hemin can catalyze the reaction of tyrosine (Tyr) and hydrogen peroxide (H2O2). Catalysis was found to effectively reduce or eliminate oxidative damage to cells induced by H2O2 or UVA. The scavenging effects of hemin for other free-radical ROS were also evaluated through pyrogallol autoxidation, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·)-scavenging assays, and phenanthroline–Fe2+ assays. The results show that a mixture of hemin and tyrosine exhibits strong scavenging activities for H2O2, superoxide anion (O2−·), DPPH·, and the hydroxyl radical (·OH). Furthermore, the inhibition of oxidative damage to human skin keratinocyte (HaCaT) cells induced by H2O2 or UVA was evaluated. The results show that catalysis can significantly reduce the ratio of cell apoptosis and death and inhibit the release of lactate dehydrogenase (LDH), as well as accumulation of malondialdehyde (MDA). Furthermore, the resistance to apoptosis was found to be enhanced. These results show that the mixture of hemin and tyrosine has a significantly protective effect against oxidative damage to HaCaT cells caused by UVA, suggesting it as a protective agent for combating UVA damage.

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“…Short wave UVC is 200‒280 nm, mediumwave UVB is 280‒320 nm, and longwave UVA is 320‒400 nm. 1 , 2 UVB has two sides. According to different irradiation doses, different disease models will have different effects.…”

Section: Introductionmentioning

confidence: 99%

UVB irradiation differential regulate miRNAs expression in skin photoaging

Fang

Chen

Wang

et al. 2022

Anais Brasileiros de Dermatologia

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An ex vivo model of human skin photoaging induced by UVA radiation compatible with summer exposure in Brazil

Cited by 15 publications

References 35 publications

Photoresponsive liposomes and LipoParticles by incorporating a photosensitizer agent in their lipid membrane

Photoresponsive liposomes and LipoParticles by incorporating a photosensitizer agent in their lipid membrane

Hemin with Peroxidase Activity Can Inhibit the Oxidative Damage Induced by Ultraviolet A

UVB irradiation differential regulate miRNAs expression in skin photoaging

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