An evolutionary recent neuroepithelial cell adhesion function of huntingtin implicates ADAM10-Ncadherin

Sardo, Valentina Lo; Zuccato, Chiara; Gaudenzi, Germano; Vitali, Barbara; Ramos, Catarina; Tartari, Marzia; Myre, Michael A.; Walker, James A.; Pistocchi, Anna; Conti, Luciano; Valenza, Marta; Drung, Binia; Schmidt, Boris; Gusella, James F.; Zeitlin, Scott; Cotelli, Franco; Cattaneo, Elena

doi:10.1038/nn.3080

Cited by 101 publications

(95 citation statements)

References 50 publications

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“…For inhibition of ADAM-10 dependent Hla-mediated lysis, peritoneal cells were pre-incubated with 80μM GI254023X (Okeanos Tech.) for 60min at 37°C followed by addition of a two-fold stock Hla leading to a final concentration of 40μM GI254023X 49 and 10μg/ml of Hla in the cell suspension which was incubated for a further 20–30 minutes. Following incubation, cells were washed in FACS buffer and stained with anti-CD19 APC-Cy7 or PE (clone 1D3), anti-CD11c PE-Cy7 (clone HL3), anti-CD11b FITC (clone M1/70) and anti-F4/80 PE (clone BM8) or A647 (clone: CI:A3-1) antibodies (all purchased from BD or AbD Serotec) and assessed by flow cytometry using 10-laser LSR II cytometer (BD).…”

Section: Methodsmentioning

confidence: 99%

Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection

et al. 2013

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Transendothelial migration of neutrophils in post-capillary venules is a key event in the inflammatory response against pathogens and tissue damage. The precise regulation of this process is incompletely understood. We report that perivascular macrophages are critical for neutrophil migration into skin infected with the pathogen Staphylococcus aureus. Using multiphoton intravital microscopy we show that neutrophils extravasate from inflamed dermal venules in close proximity to perivascular macrophages, which are a major source of neutrophil chemoattractants. The virulence factor alpha-hemolysin lyses perivascular macrophages leading to decreased neutrophil transmigration. Our data illustrate a previously unrecognized role for perivascular macrophages in neutrophil recruitment to inflamed skin, and indicate that Staphylococcus aureus uses hemolysin-dependent killing of these cells as an immune evasion strategy.

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Section: Methodsmentioning

confidence: 99%

Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection

et al. 2013

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“…However, it should be considered that long-term regulation of at least ADAM10 and ADAM17 will be challenging, given the pronounced phenotypes of ADAM10 and 17 knockout mouse models. Development 139 (20) by the regulation of ADAM10 and N-cadherin (cadherin 2) shedding (Lo Sardo et al, 2012). …”

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confidence: 99%

Ectodomain shedding and ADAMs in development

Weber¹,

2012

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SummaryProteolytic enzymes belonging to the A Disintegin And Metalloproteinase (ADAM) family are able to cleave transmembrane proteins close to the cell surface, in a process referred to as ectodomain shedding. Substrates for ADAMs include growth factors, cytokines, chemokines and adhesion molecules, and, as such, many ADAM proteins play crucial roles in cell-cell adhesion, extracellular and intracellular signaling, cell differentiation and cell proliferation. In this Review, we summarize the fascinating roles of ADAMs in embryonic and adult tissue development in both vertebrates and invertebrates. Key words: ADAM, Notch, Ectodomain shedding, Cell fate determination, DifferentiationIntroduction Proteins belonging to the 'A Disintegrin And Metalloproteinase' (ADAM) family are membrane-anchored proteases that are able to cleave the extracellular domains of membrane-bound proteins in a process known as 'ectodomain shedding'. Typical substrates of ADAM proteases are growth factors, cytokines, chemokines and their receptors, as well as cell adhesion molecules and differentiation factors (Reiss and Saftig, 2009). ADAMs were initially discovered as novel type I transmembrane proteins with homology to snake venom integrin ligands and that played a functional role during guinea-pig sperm-egg fusion (Blobel et al., 1992). Subsequently, approximately half of the ADAM family members were predicted and then shown to possess zinc-dependent protease activity related to that exhibited by adamalysins metallopeptidases (Wolfsberg et al., 1993; Huxley-Jones et al., 2007). These active 'sheddases ' (ADAM8, 9, 10, 12, 15, 17, 19, 20, 21, 28, 30 and 33) share a typical consensus sequence (HEXGHXXGXXHD) that is present in zinc-binding proteases (Bode et al., 1993). So far, 40 family members have been identified in the mammalian genome (Puente and Lopez-Otin, 2004; Edwards et al., 2008), of which 37 are expressed in mice (most of them in a testis-specific manner) and 22 are thought to be expressed in humans (Table 1). In both mouse and human, intronless coding sequences probably representing pseudogenes (e.g. human ADAM5P and ADAM6) have also been described. The expression of ADAM or ADAM-related proteins has also been described in many different species, including the yeast Schizosaccharomyces pombe, the nematode worm Caenorhabditis elegans, the frog Xenopus laevis, the zebrafish Danio rerio and the fruitfly Drosophila melanogaster (Alfandari et al., 1997;Nakamura et al., 2004; Huxley-Jones et al., 2007; Iida et al., 2010). It was quickly realized that ADAMs are widely expressed and play fundamental roles during developmental processes, by regulating cell-cell and cell-matrix interactions and by modulating differentiation, migration, receptor-ligand signaling or repulsion (Becherer and Blobel, 2003).Following studies of the catalytically active ADAMs, ectodomain shedding emerged as a central biological event. This proteolytic process primarily affects type I and type II transmembrane proteins, although glycosylphosphatidylinisot...

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“…1C), indicating that the survival of oocytes was not affected by inhibiting ADAM10. In addition, we cultured ovaries with another ADAM10 selective inhibitor GI254023X (Hoettecke et al, 2010;Hundhausen et al, 2003;Lo Sardo et al, 2012). The results showed that 7 days of GI254023X treatment also significantly decreased the number of follicles (1138.0±184.0 per ovary for GI254023X versus 8188.0±1152.3 per ovary for control; Fig.…”

Section: Suppressing Adam10 Disrupts Germline Cyst Breakdown and Primmentioning

confidence: 88%

ADAM10–Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice

Feng

Wang

Cai

et al. 2016

Journal of Cell Science

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Ovarian follicles are the basic functional units of female reproduction in the mammalian ovary. We show here that the protein a disintegrin and metalloproteinase domain 10 (ADAM10), a cell surface sheddase, plays an indispensable role in controlling primordial follicle formation by regulating the recruitment of follicle supporting cells in mice. We demonstrate that suppressing ADAM10 in vitro or deletion of Adam10 in vivo disrupts germline cyst breakdown and primordial follicle formation. Using a cell lineage tracing approach, we show that ADAM10 governs the recruitment of ovarian follicle cells by regulating the differentiation and proliferation of LGR5-positive follicle supporting progenitor cells. By detecting the development of FOXL2-positive pregranulosa cells, we found that inhibiting ADAM10 reduced the number of FOXL2-positive cells in perinatal ovaries. Furthermore, inhibiting ADAM10 suppressed the activation of Notch signaling, and blocking Notch signaling also disrupted the recruitment of follicle progenitor cells. Taken together, these results show that ADAM10-Notch signaling in ovarian somatic cells governs the primordial follicle formation by controlling the development of ovarian pregranulosa cells. The proper recruitment of ovarian follicle supporting cells is essential for establishment of the ovarian reserve in mice.

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An evolutionary recent neuroepithelial cell adhesion function of huntingtin implicates ADAM10-Ncadherin

Cited by 101 publications

References 50 publications

Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection

Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection

Ectodomain shedding and ADAMs in development

ADAM10–Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice

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