An Evolutionarily Conserved Receptor-like Kinases Signaling Module Controls Cell Wall Integrity During Tip Growth

Westermann, Jens; Streubel, Susanna; Franck, Christina Maria; Lentz, Roswitha; Dolan, Liam; Boisson-Dernier, Aurélien

doi:10.1016/j.cub.2019.09.069

Cited by 22 publications

(12 citation statements)

References 50 publications

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“…We transiently co-expressed the fluorescent protein fusions AtMRI-YFP, MpFER-YFP, and MpMRI-YFP with AtNPSN12-mCherry. While MpFER-YFP showed signal exclusive to the plasma membrane, AtMRI and MpMRI displayed plasma membrane localization with traces in the cytoplasm as reported before (Figure 3) (Boisson-Dernier et al, 2015;Westermann et al, 2019).…”

Section: Plasma Membranesupporting

confidence: 80%

“…The coding sequences of interest were cloned into Gateway (GW)-compatible entry vectors, pDONR201 and pDONR207 (Invitrogen), and then remobilized to be integrated in the respective GW destination vectors (Supplementary Table S1). The cloning procedure was as described before (Westermann et al, 2019).…”

Section: Cloning Of Dna Constructsmentioning

confidence: 99%

“…cytoplasmic kinase of the PTI1-like family, AtMRI, while the AtANX1 homolog AtFERONIA (AtFER) acts upstream of AtMRI during root hair growth control (Boisson-Dernier et al, 2015). We showed recently that tip-growth control in Marchantia rhizoids relies on an evolutionarily conserved signaling module comprised of the unique Marchantia MLR MpFER and its downstream component and unique Marchantia PTI1-like MpMRI (Westermann et al, 2019). We transiently co-expressed the fluorescent protein fusions AtMRI-YFP, MpFER-YFP, and MpMRI-YFP with AtNPSN12-mCherry.…”

Section: Plasma Membranementioning

confidence: 99%

“…In the last decade, the liverwort Marchantia polymorpha has emerged as a powerful model system to study early land plant evolution due to its early evolutionary divergence in the land plant phylogenetic tree (Shaw et al, 2011;Harrison, 2017;Morris et al, 2018). Research deploying M. polymorpha has led to a series of insightful studies on the functional evolution of abscisic acid (ABA, Lind et al, 2015;Eklund et al, 2018) and jasmonic acid (JA) signaling mechanisms (Monte et al, 2018(Monte et al, , 2019Peñuelas et al, 2019), plant immunity (Carella et al, 2019;Gimenez-Ibanez et al, 2019), reproductive and vegetative development (Flores-Sandoval et al, 2015;Proust et al, 2016;Jones and Dolan, 2017;Rövekamp et al, 2016;Otani et al, 2018;Westermann et al, 2019;Thamm et al, 2020) and cell division (Buschmann et al, 2016). It offers the advantage of genetic and morphological simplicity in combination with its dominant haploid vegetative life phase, allowing for fast generation of knockout mutants and subsequent phenotypic analyses, irrespectively of timeconsuming homozygous mutant generation (Ishizaki et al, 2015b).…”

Section: Introductionmentioning

confidence: 99%

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A Comprehensive Toolkit for Quick and Easy Visualization of Marker Proteins, Protein–Protein Interactions and Cell Morphology in Marchantia polymorpha

et al. 2020

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Even though stable genomic transformation of sporelings and thalli of Marchantia polymorpha is straightforward and efficient, numerous problems can arise during critical phases of the process such as efficient spore production, poor selection capacity of antibiotics or low transformation efficiency. It is therefore also desirable to establish quick methods not relying on stable transgenics to analyze the localization, interactions and functions of proteins of interest. The introduction of foreign DNA into living cells via biolistic mechanisms has been first reported roughly 30 years ago and has been commonly exploited in established plant model species such as Arabidopsis thaliana or Nicotiana benthamiana. Here, we report the fast and reliable transient biolistic transformation of Marchantia thallus epidermal cells using fluorescent protein fusions. We present a catalog of fluorescent markers which can be readily used for tagging of a variety of subcellular compartments. Moreover, we report the functionality of the bimolecular fluorescence complementation (BiFC) in M. polymorpha with the example of the p-body markers MpDCP1/2. Finally, we provide standard staining procedures for live cell imaging in M. polymorpha, applicable to visualize cell boundaries or cellular structures, to complement or support protein localizations and to understand how results gained by transient transformations can be embedded in cell architecture and dynamics. Taken together, we offer a set of easy and quick tools for experiments that aim at understanding subcellular localization, protein-protein interactions and thus functions of proteins of interest in the emerging early diverging land plant model M. polymorpha.

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Section: Plasma Membranesupporting

confidence: 80%

Section: Cloning Of Dna Constructsmentioning

confidence: 99%

Section: Plasma Membranementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Comprehensive Toolkit for Quick and Easy Visualization of Marker Proteins, Protein–Protein Interactions and Cell Morphology in Marchantia polymorpha

et al. 2020

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“…Lrx1 mutants develop aberrant root hairs, and TOR signaling can reverse the phenotype by changing the cell wall composition in the same way as it is detected in rol5 mutants [193]. The maintenance of cell wall integrity (CWI) during controlled root hair tip expansion is crucial to prevent the expanding root hair tip from bursting [89,194]. The PM located kinase FERONIA (FER) modulates the positioning of ROP2, followed by stabilizing actin filaments at the PM [167,195].…”

Section: Membrane Composition and Endomembrane Trafficking Is Fine-tuned During Root Hair Growthmentioning

confidence: 99%

The TOR–Auxin Connection Upstream of Root Hair Growth

Retzer

Weckwerth

2021

Plants

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Plant growth and productivity are orchestrated by a network of signaling cascades involved in balancing responses to perceived environmental changes with resource availability. Vascular plants are divided into the shoot, an aboveground organ where sugar is synthesized, and the underground located root. Continuous growth requires the generation of energy in the form of carbohydrates in the leaves upon photosynthesis and uptake of nutrients and water through root hairs. Root hair outgrowth depends on the overall condition of the plant and its energy level must be high enough to maintain root growth. TARGET OF RAPAMYCIN (TOR)-mediated signaling cascades serve as a hub to evaluate which resources are needed to respond to external stimuli and which are available to maintain proper plant adaptation. Root hair growth further requires appropriate distribution of the phytohormone auxin, which primes root hair cell fate and triggers root hair elongation. Auxin is transported in an active, directed manner by a plasma membrane located carrier. The auxin efflux carrier PIN-FORMED 2 is necessary to transport auxin to root hair cells, followed by subcellular rearrangements involved in root hair outgrowth. This review presents an overview of events upstream and downstream of PIN2 action, which are involved in root hair growth control.

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The maturation and aging trajectory of Marchantia polymorpha at single-cell resolution

et al. 2023

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An Evolutionarily Conserved Receptor-like Kinases Signaling Module Controls Cell Wall Integrity During Tip Growth

Cited by 22 publications

References 50 publications

A Comprehensive Toolkit for Quick and Easy Visualization of Marker Proteins, Protein–Protein Interactions and Cell Morphology in Marchantia polymorpha

A Comprehensive Toolkit for Quick and Easy Visualization of Marker Proteins, Protein–Protein Interactions and Cell Morphology in Marchantia polymorpha

The TOR–Auxin Connection Upstream of Root Hair Growth

The maturation and aging trajectory of Marchantia polymorpha at single-cell resolution

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