and Sacks1' 2, 3 have shown that when the enamel surl faces of both nondecalcified teeth and enamel-dentin fragments were treated with an acidulated fluoride solution and immersed in broth seeded with Lactobacillus acidophilus for thirty days, decalcification of enamel was reduced from 31.69 to 39.37 per cent. In a previous report3 it has been shown that when 2 per cent sodium fluoride solution was applied to both previously decalcified teeth and enamel-dentin fragments, it was as effective for reducing further decalcification of enamel as when applied either to nondecalcified teeth or nondecalcified enamel-dentin fragments. The favorable results achieved with decalcified teeth have suggested variation in technic for applying the solutions, with greater promise for reducing the susceptibility of enamel to decaleification Volker,4 Bibby,5 Muhler and Van Huysen,6 Phillips,7 and Muhler, Boyd and Van Huysen8 have shown with either powdered enamel or enamel fragments that both were less soluble to acids following their treatment with a fluoride solution. Phillips7 has shown that the hardness and impermeability of enamel was increased following exposure to a fluoride solution. Likins and McClure9 in their studies of powdered enamel treated with various fluoride solutions suggest that the washing of such enamel in distilled water removed the adsorbed fluorides. Scott, Picard, and Wyckofft0 have reported that CaF2 deposits formed upon slabs of enamel during their immersion in a fluoride solution can be removed by washing in distilled water.The purpose of this investigation has been to determine the efficacy of both alkaline and acidulated fluoride solutions for reducing decalcification* of enamel.
METHOD OF STUDYSixty-one noncarious extracted molar teeth, representing fifty-two adults, were thoroughly cleaned with scalers, rubber cups, and pumice. With the aid of separating disks the crown of each tooth, after being removed from the root at the cemento-enamel junction, was cut into four similar parts known as fragments A, B, C, and D. The enamel-dentin fragments were then washed for a few seconds in running tap water in order to remove any tooth dust or foreign particles present. The 244 fragments were then dried at 420 C. for six