An evaluation of the tolerance of the autotransplanted canine lung against warm ischemia

Yamazaki, Fumio; Wada, Hiromi; Aoki, Minoru; Inui, Kentaro; Hitomï, Shigeki

doi:10.1007/bf02471409

Cited by 16 publications

(5 citation statements)

References 14 publications

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“…Palazzo et al [20] demonstrated in a model of left lung ischemia-reperfusion in dogs that a period of 120 min of warm ischemia results in signifi cant lung injury also of the contralateral lung, but this was not at all close to being lethal. These fi ndings are similar to others, which limit the maximum safe warm ischemic period of defl ated lungs to 2 h [6,[21][22][23] . However, all authors investigated lung ischemia at room temperature (20-22 ° C) resulting in signifi cantly lower organ temperatures.…”

Section: Discussionsupporting

confidence: 71%

“…Within this period we found that 90 min of lung ischemia at 37 ° C results in signifi cant and reproducible reperfusion injury in all investigated parameters ( fi g. [3][4][5][6]. Animals of the I-60 group did show lung injury at the start of reperfusion but recovered more and more during the reperfusion period of 2 h ( fi g. 2-4 ).…”

Section: Discussionmentioning

confidence: 93%

“…Warm ischemic tolerance of the nonventilated lung is described to be up to several hours [5][6][7] . Within this period we found that 90 min of lung ischemia at 37 ° C results in signifi cant and reproducible reperfusion injury in all investigated parameters ( fi g. [3][4][5][6].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we presumed that the animal in a supine position has a more homogenous perfusion and ventilation on each lung side in comparison to side positioning, especially after injury of one side [2,6,20] .…”

Section: Discussionmentioning

confidence: 99%

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Monitoring of Lung Edema by Microwave Reflectometry during Lung Ischemia-Reperfusion Injury in vivo

et al. 2006

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It is still unclear whether lung edema can be monitored by microwave reflectometry and whether the measured changes in lung dry matter content (DMC) are accompanied by changes in PaO₂ and in pro- to anti-inflammatory cytokine expression (IFN-γ and IL-10). Right rat lung hili were cross-clamped at 37°C for 0, 60, 90 or 120 min ischemia followed by 120 min reperfusion. After 90 min (DMC: 15.9 ± 1.4%; PaO₂: 76.7 ± 18 mm Hg) and 120 min ischemia (DMC: 12.8 ± 0.6%; PaO₂: 43 ± 7 mm Hg), a significant decrease in DMC and PaO₂ throughout reperfusion compared to 0 min ischemia (DMC: 19.5 ± 1.11%; PaO₂: 247 ± 33 mm Hg; p < 0.05) was observed. DMC and PaO₂ decreased after 60 min ischemia but recovered during reperfusion (DMC: 18.5 ± 2.4%; PaO₂: 173 ± 30 mm Hg). DMC values reflected changes on the physiological and molecular level. In conclusion, lung edema monitoring by microwave reflectometry might become a tool for the thoracic surgeon.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Monitoring of Lung Edema by Microwave Reflectometry during Lung Ischemia-Reperfusion Injury in vivo

et al. 2006

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“…Yamaz aki et al (17) rep orted in the eanine lung autotransplanta tion model that grafts showing low Pao, immedi ately after reperfusion revealed unsatisfaetory pulmonary funetion on da y 2 or 6 after transplantation. Then, the adequat e Pa a , level of th e transplante d lung immediately after re perfus ion was th ought th e first target.…”

Section: Discussionmentioning

confidence: 98%

Effects of Trehalose in Preservation of Canine Lung for Transplants

Hirata¹,

Yokomise²,

Fukuse³

et al. 1993

Thorac cardiovasc Surg

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The effect of trehalose, a non-reducing disaccharide which stabilizes and protects membranes, in the preservation of canine lungs was examined when Euro-Collins solution was basically used as a preservant. In group I, five lungs were perfused and preserved in an Euro-Collins solution modified by replacing the glucose with 35.0 g/L of trehalose. Five control lungs (group II) were perfused and preserved with Euro-Collins solution containing 35.0 g/L glucose. In both groups, no vasodilators were used. After preservation for 12 hours, left lung allotransplantation was performed. At 10, 40, 70, and 130 minutes after reperfusion, the right pulmonary artery was clamped for 10 minutes and four parameters were measured: arterial oxygen tension, mean pulmonary arterial pressure, peak inspiratory pressure, and wet/dry weight ratios. The transplanted lung was also examined histologically. At 10, 40, 70, and 130 minutes after reperfusion, oxygen-tension levels from group I were 263.2 +/- 19.2, 283.4 +/- 14.0, 277.5 +/- 19.9 and 264.9 +/- 26.2 mmHg, respectively. In group II, the corresponding values were 191.2 +/- 33.9, 188.0 +/- 40.3, 153.4 +/- 40.0 and 134.7 +/- 49.4 mmHg, respectively. At 70 and 130 minutes the difference were significant (p < 0.05). All transplanted lungs from group I showed normal histology, whereas four dogs in group II developed severe pulmonary edema and one had a partially edematous lung. These findings suggest that simple substitution of trehalose for glucose has a beneficial effect on preservation of canine lung for 12 hours.

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Expression of the adult T-cell leukemia-derived factor, human thioredoxin, in the allotransplanted canine lung

Muro

Hirata

et al. 1995

Surg Today

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The relationship between the expression of adult T-cell leukemia-derived factor, human thioredoxin (ADF/TRX), and rejection in transplanted canine lungs was investigated in this study. Of a total 27 adult mongrel dogs, 24 underwent allotransplantation of the left lung with no immunosuppressant and the other three underwent autotransplantation of the left lung. Of the allotransplanted dogs, five were killed on postoperative day (POD) 1, five on POD 2, seven on POD 3, and seven on POD 5, while all three autotransplanted dogs were killed on POD 5. Histological examination was performed on the 24 allotransplanted left lungs (group A), 12 autologous right lungs (group B), and bilateral lungs of the three autotransplanted dogs (group C). The lung tissue was stained with anti-ADF antibody, and the high-ADF-producing cells (ADFh cells) in a randomly chosen field were counted as an index of ADF expression. As the signs of rejection in the group A lungs became more severe with time, the ADFh cells increased in number: 1.68 +/- 1.15, 6.08 +/- 3.44, 14.03 +/- 6.09, and 47.74 +/- 18.89, on PODs 1, 2, 3, and 5, respectively. However, the number of ADFh cells in the group B and group C lungs did not become significantly different from that on POD 1 in group A. These results suggested that ADF/TRX expression may be useful for the early diagnosis of rejection of transplanted lungs.

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An evaluation of the tolerance of the autotransplanted canine lung against warm ischemia

Cited by 16 publications

References 14 publications

Monitoring of Lung Edema by Microwave Reflectometry during Lung Ischemia-Reperfusion Injury in vivo

Monitoring of Lung Edema by Microwave Reflectometry during Lung Ischemia-Reperfusion Injury in vivo

Effects of Trehalose in Preservation of Canine Lung for Transplants

Expression of the adult T-cell leukemia-derived factor, human thioredoxin, in the allotransplanted canine lung

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