An Evaluation of the Sysmex NE-8000® Hematology Analyzer

Abstract

The Sysmex NE-8000 is a new, fully automated hematology analyzer capable of providing a five-part white blood cell differential count and identifying abnormal specimens. This instrument was evaluated on 5,000 consecutive blood specimens and compared to the Coulter S Plus-IV analyzer and manual differential cell counts to determine the efficacy of its five-cell differential and screening capabilities. There was a high correlation between the commercial counters for the standard parameters, white blood cell count, red blood cell count, hemoglobin level, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, and platelet count (r greater than 0.95), except for the mean corpuscular hemoglobin concentration (r = 0.51), for which the NE-8000 was considered the more accurate measurement. Precision and linearity studies were excellent. The white blood cell count, red blood cell count, hemoglobin level, and platelet count were reproducible on specimens stored at 4 degrees C or room temperature for 72 hours and the differential counts were reproducible for 12 hours. The correlations between automated and manual counts for neutrophils, eosinophils, basophils, and lymphocytes were excellent: r = 0.912, 0.945, 0.332, and 0.964, respectively. The monocyte correlation improved with software modification from 0.306 to 0.801. The NE-8000 gave accurate and reproducible differential counts for neutrophils, lymphocytes, and monocytes on specimens with white blood cell counts as low as 0.6 x 10(9)/1. The ability of the instrument to 'flag' abnormal specimens was excellent. The false-positive rate on normal samples was 1.8%, and the false-negative rate on known abnormal samples was 0.3%, due only to nonrecognition of a mild left shift. The identification of specific abnormalities was less precise. The NE-8000 is a powerful hematology analyzer that can perform a five-part white blood cell differential count accurately for a wide range of WBCs and reliably indicate abnormal specimens. It is an excellent screening tool for distinguishing between normal and abnormal specimens and identifying those that require microscopy. Its reliability significantly reduces the need for manual film examination.

Influence of Storage Time and Temperature on Haematological Measurands using a Sysmex NE 8000 Analyser. Einfluß von Lagerungszeit und -temperatur von hämatologischen Parametern bei Verwendung eines Sysmex NE 8000 Analysers

“…However, we felt that the limits for imprecision and stability derived from the recommendation of the German Medical Association for each analyte better reflect clinical requirements than from intra-individual diurnal variation. Even new analysers did not meet the analytical goal of 1/12 of the difference of reference limits for all analytes [7][8][9][10], hence we used a wider limit of 1/6 the difference for comparison (Tables 1 and 2).…”

“…They found no statistically significant change in analytes with storage at 4°C for 72 h and blood stored at room temperature showed a significant increase in MCV, haematocrit and MCHC in 24 hours. Hallwell et al describe a maximum storage time of 72 h for all parameters at 4 °C and room temperature (9). But in 1975, Lawrence et al already note an increase of MCV after storage of blood overnight at room temperature compared with storage at 4 °C [13].…”

Erroneous Monocyte and Neutrophil Counts with the Sysmex NE-1500 in Patients Recovering from Bone Marrow Aplasia

“…Brought to you by | University of Arizona Authenticated Download Date | 5/30/15 11:29 PM Sysmex NE-1500 was found to perform satisfactorily (1)(2)(3)(4)(5)(6).…”

“…Although it was extensively evaluated and found to perform well in our laboratory and in other institutions (1)(2)(3)(4)(5)(6), it was only recently, that it became clear that unexpected and erroneous results in differential counts were being generated, especially in samples from patients recovering from bone marrow aplasia. It provides both complete blood count and fivepart differential counts.…”

The Characterisation of Leukaemias with the Sysmex NE-8000

“…Evalua tion studies of this apparatus have previously been de scribed [1][2][3]. The scattergrams and histograms generated by the NE-8000 may provide a novel way of evaluating and interpreting the white cell population in leukaemia.…”