An evaluation of the methods available for analysing results from migration inhibitory factor (MIF) test

Ja, Morris; Stevens, A.E.; Hebert, C. Nancy

doi:10.1016/0022-1759(76)90049-1

Cited by 7 publications

(3 citation statements)

References 8 publications

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“…U' ) The area of cell tnigration is usually quantified by tracing a magnification of it on paper, followed by planimetric measurement, or by cutting out and weighing the tracing (see 38). Experience shows that a more stringent statisticai treatment of the data than the above-mentioned is highly desirable (6,54,56,58) and also that measurement of migration distance instead of migration area is justified (85). The efiect is often expressed as the migration index (Mil, which is the ratio of mean areas ol migration in the presence of antigen to those in the absence of antigen.…”

Section: -14 Daysmentioning

confidence: 99%

“…Variability between identically treated capillaries could be large; therelore a sufficient number of capillaries should be used for each reagent (6,38,56). At measurements of areas, square root transformation of data normalizes the distribution and reduces the scatter of the values (58 Fig. If MI is less than 0.8, many authors conclude that the effect is significant.…”

Section: -14 Daysmentioning

confidence: 99%

“…Experience shows that a more stringent statisticai treatment of the data than the above-mentioned is highly desirable (6,54,56,58) and also that measurement of migration distance instead of migration area is justified (85). At measurements of areas, square root transformation of data normalizes the distribution and reduces the scatter of the values (58 Fig. 2 gives a schematic representation of various factors shown to influence the production and biological properties of antigenand mitogen-induced guinea pig MIF.…”

Section: Metkods Of Oisaymentioning

confidence: 99%

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Lymphocyte‐Derived Factors Affecting Cell Migration In Vitro

Bergstrand

1979

Allergy

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Section: -14 Daysmentioning

confidence: 99%

Section: -14 Daysmentioning

confidence: 99%

Section: Metkods Of Oisaymentioning

confidence: 99%

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Lymphocyte‐Derived Factors Affecting Cell Migration In Vitro

Bergstrand

1979

Allergy

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Cell‐mediated immune reactivity of sinclair melanoma‐bearing swine to 3 M KC1 extracts of swine and human melanoma

Hook

Hamby

Millikan

et al. 1983

Intl Journal of Cancer

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Leukocyte migration inhibition (LMI) assays were performed using 3 M KCI extracts to detect cell-mediated immune reactions against tumor-associated antigens (TAA) of swine melanoma. Positive LMI reactivity was 70% or greater in melanoma-bearing swine compared to 10% reactivity or less in normal swine tested with extracts of either fresh or tissue-cultured swine melanoma cells. Positive LMI reactivity was 10% or less in both melanoma and normal swine tested with extracts of normal fetal and adult swine tissues. Extracts of tissue-cultured swine melanoma and human melanoma cells were equally capable of stimulating positive LMI reactivity in melanoma swine and human melanoma patients; normal swine and human donors demonstrated 10% or less positive LMI reactivity to these extracts. Positive LMI reactivity was not stimulated in swine melanoma or human melanoma donors by extracts of tissue-cultured human breast tumor cells or fresh and tissue-cultured mouse B16 melanoma cells. Evaluation of these results indicate that melanoma-bearing swine develop cell-mediated immune reactivity toward TAA on swine melanomas and, inasmuch as LMI assays were performed with allogeneic extracts, suggests the presence of common TAA on swine melanomas. Furthermore, these data suggest that swine and human melanoma cells may share some common TAA and that this common TAA is not expressed on mouse B16 melanoma cells.

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