An evaluation of the endophytic colonies present in pathogenic and non-pathogenic Vanguerieae using electron microscopy

Stanton, S.L.; Meyer, Jacqueline; Merwe, C.F. van der

doi:10.1016/j.sajb.2013.01.007

Cited by 6 publications

(5 citation statements)

References 9 publications

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“…The cellular morphology of BUN14 was visualized using a JCM-5700 Scanning Electron Microscope, resolution 0.6 nm, specimen size 5 mm ∅ × 0.6 mm high, with a Gatan Digital Micrograph imaging system and SE & BS detectors 53 . BUN14 cultures were centrifuged (10 min 14,000 g ) and bacterial biomass was fixed in 2.5% gluteraldehyde in 0.075 M K-phosphate buffer (pH 7.4) for 2 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…BUN14 cultures were centrifuged (10 min 14,000 g ) and bacterial biomass was fixed in 2.5% gluteraldehyde in 0.075 M K-phosphate buffer (pH 7.4) for 2 h at room temperature. The preparation of the Scanning Electron Microscope (SEM) was performed according to Stanton et al 53 . To test the ability of strain BUN14 to utilize hydrocarbon substrates as the sole carbon and energy source, the organism was inoculated onto solid ONR7a agar media containing individual hydrocarbons (pristane, phenanthrene, pyrene, naphthalene, fluoranthene, dibenzothiophene, dibenzofuran, squalene, and carbazole: 50 mM final concentration) and incubated for 7 days at 30 ± 1 °C.…”

Section: Methodsmentioning

confidence: 99%

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Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14

Mahjoubi

Aliyu

Neifar

et al. 2021

Sci Rep

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Bioremediation offers a viable alternative for the reduction of contaminants from the environment, particularly petroleum and its recalcitrant derivatives. In this study, the ability of a strain of Pseudomonas BUN14 to degrade crude oil, pristane and dioxin compounds, and to produce biosurfactants, was investigated. BUN14 is a halotolerant strain isolated from polluted sediment recovered from the refinery harbor on the Bizerte coast, north Tunisia and capable of producing surfactants. The strain BUN14 was assembled into 22 contigs of 4,898,053 bp with a mean GC content of 62.4%. Whole genome phylogeny and comparative genome analyses showed that strain BUN14 could be affiliated with two validly described Pseudomonas Type Strains, P. kunmingensis DSM 25974T and P. chloritidismutans AW-1T. The current study, however, revealed that the two Type Strains are probably conspecific and, given the priority of the latter, we proposed that P. kunmingensis DSM 25974 is a heteronym of P. chloritidismutans AW-1T. Using GC-FID analysis, we determined that BUN14 was able to use a range of hydrocarbons (crude oil, pristane, dibenzofuran, dibenzothiophene, naphthalene) as a sole carbon source. Genome analysis of BUN14 revealed the presence of a large repertoire of proteins (154) related to xenobiotic biodegradation and metabolism. Thus, 44 proteins were linked to the pathways for complete degradation of benzoate and naphthalene. The annotation of conserved functional domains led to the detection of putative genes encoding enzymes of the rhamnolipid biosynthesis pathway. Overall, the polyvalent hydrocarbon degradation capacity of BUN14 makes it a promising candidate for application in the bioremediation of polluted saline environments.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14

Mahjoubi

Aliyu

Neifar

et al. 2021

Sci Rep

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“…Cultures of BU33N were centrifuged (10 min 14.000 g) and bacterial biomass was fixed in 2.5% gluteraldehyde in 0.075 M K-phosphate buffer (pH 7.4) for 2 h at room temperature. The preparation of the Scanning Electron Microscope (SEM) was performed according to Stanton et al [26], and samples visualized using a JCM-5700 Scanning Electron Microscope, resolution 0.6nm, specimen size 5 mm ∅ × 0.6 mm high, with a Gatan Digital Micrograph imaging system and SE & BS detectors.…”

Section: Methodsmentioning

confidence: 99%

The genome of Alcaligenes aquatilis strain BU33N: Insights into hydrocarbon degradation capacity

Mahjoubi¹,

Aliyu

Cappello

et al. 2019

PLoS ONE

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Environmental contamination with hydrocarbons though natural and anthropogenic activities is a serious threat to biodiversity and human health. Microbial bioremediation is considered as the effective means of treating such contamination. This study describes a biosurfactant producing bacterium capable of utilizing crude oil and various hydrocarbons as the sole carbon source. Strain BU33N was isolated from hydrocarbon polluted sediments from the Bizerte coast (northern Tunisia) and was identified as Alcaligenes aquatilis on the basis of 16S rRNA gene sequence analysis. When grown on crude oil and phenanthrene as sole carbon and energy sources, isolate BU33N was able to degrade ~86%, ~56% and 70% of TERHc, n-alkanes and phenanthrene, respectively. The draft genome sequence of the A. aquatilis strain BU33N was assembled into one scaffold of 3,838,299 bp (G+C content of 56.1%). Annotation of the BU33N genome resulted in 3,506 protein-coding genes and 56 rRNA genes. A large repertoire of genes related to the metabolism of aromatic compounds including genes encoding enzymes involved in the complete degradation of benzoate were identified. Also genes associated with resistance to heavy metals such as copper tolerance and cobalt-zinc-cadmium resistance were identified in BU33N. This work provides insight into the genomic basis of biodegradation capabilities and bioremediation/detoxification potential of A. aquatilis BU33N.

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“…For scanning electron microscopy, BU72 cultures were grown in Tryptic Soy Broth (TSB) for 48 at 28°C until exponential growth phase (OD600 around 0.5). Preparations for scanning electron microscope were performed according to Stanton et al (2013). Cultures was were/was centrifuged and bacterial biomass was xed in 2.5% glutaraldehyde in K-phosphate buffer (0.075 M, pH 7.4) for 2 h at room temperature.…”

Section: Characterization Of Bu72 By Scanning Electron Microscopy And...mentioning

confidence: 99%

“…The Scanning Electron Microscope (SEM) used was a JCM-5700SEM, resolution 0.6 nm, specimen size 5 mm dia x 0.6 mm high, heating (1200°C) and cooling (N2(l)) stages, Gatan Digital Micrograph imaging system, SE & BS detectors (Stanton et al 2013).…”

Section: Characterization Of Bu72 By Scanning Electron Microscopy And...mentioning

confidence: 99%

Brucella pituitosa strain BU72, a new hydrocarbonoclastic bacterium through exopolysaccharide-based surfactants production

MAHJOUBI,

CHERIF,

ALIYU

et al. 2023

Preprint

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Hydrocarbons and heavy metals pollution is considered among the most prevalent and serious problems in environment due to their toxicity and persistence. Bioremediation, using microorganisms, is considered as one of the most effective ways to reduce this type of pollution. In the present study, we unveil the bioremediation potential of Brucella pituitosa strain BU72. Besides its ability to grow on multiple hydrocarbons as sole carbon source and highly tolerant to several heavy metals, BU72 was able to produce different exopolysaccharide-based surfactants (EBS) when grown with glucose or with crude oil as sole carbon source. These EBS demonstrated particular and specific functional groups as determined by Fourier transform infrared (FTIR) spectral analysis that showed a strong absorption peak at 3250 cm− 1 generated by -OH group for both EBS. The major differences in the FTIR spectra, of EBS produced are the increase of functional groups and the protein content. To better understand the EBS production coupled to the degradation of hydrocarbons and heavy metal resistance, the genome of strain BU72 was sequenced. Annotation of the genome revealed the presence of multiple genes putatively involved in EBS production pathways coupled with resistance to heavy metals genes such as arsenic tolerance and cobalt-zinc-cadmium resistance were identified. The genome sequence analysis showed the potential of BU72 to synthesize secondary metabolites and confirmed the presence of genes involved in plant-growth promoting. Here we provide a physiological and metabolic characterization associated with genomic analyses of BU72 considered as a promising candidate for application in the bioremediation.

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An evaluation of the endophytic colonies present in pathogenic and non-pathogenic Vanguerieae using electron microscopy

Cited by 6 publications

References 9 publications

Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14

Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14

The genome of Alcaligenes aquatilis strain BU33N: Insights into hydrocarbon degradation capacity

Brucella pituitosa strain BU72, a new hydrocarbonoclastic bacterium through exopolysaccharide-based surfactants production

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