An evaluation of the antioxidant properties of iodinated radiographic contrast media: An in vitro study

Tungjai, Montree; Sukantamala, Singhadeth; Malasaem, Pimchanok; Dechsupa, Nathupakorn; Kothan, S.

doi:10.1016/j.toxrep.2018.08.012

Cited by 8 publications

(3 citation statements)

References 34 publications

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“…We speculate that the antioxidant activity of iodixanol rather might be the reason for the lack of aggregation. Iodixanol was reported to exhibit antioxidant properties in in vitro free radical generating reactions and in the assays evaluating antioxidant activity of non-enzymatic antioxidants 34 , 35 .…”

Section: Discussionmentioning

confidence: 99%

A new insight into aggregation of oncolytic adenovirus Ad5-delta-24-RGD during CsCl gradient ultracentrifugation

Stepanenko

Сосновцева

Valikhov

et al. 2021

Sci Rep

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Two-cycle cesium chloride (2 × CsCl) gradient ultracentrifugation is a conventional approach for purifying recombinant adenoviruses (rAds) for research purposes (gene therapy, vaccines, and oncolytic vectors). However, rAds containing the RGD-4C peptide in the HI loop of the fiber knob domain tend to aggregate during 2 × CsCl gradient ultracentrifugation resulting in a low infectious titer yield or even purification failure. An iodixanol-based purification method preventing aggregation of the RGD4C-modified rAds has been proposed. However, the reason explaining aggregation of the RGD4C-modified rAds during 2 × CsCl but not iodixanol gradient ultracentrifugation has not been revealed. In the present study, we showed that rAds with the RGD-4C peptide in the HI loop but not at the C-terminus of the fiber knob domain were prone to aggregate during 2 × CsCl but not iodixanol gradient ultracentrifugation. The cysteine residues with free thiol groups after the RGD motif within the inserted RGD-4C peptide were responsible for formation of the interparticle disulfide bonds under atmospheric oxygen and aggregation of Ad5-delta-24-RGD4C-based rAds during 2 × CsCl gradient ultracentrifugation, which could be prevented using iodixanol gradient ultracentrifugation, most likely due to antioxidant properties of iodixanol. A cysteine-to-glycine substitution of the cysteine residues with free thiol groups (RGD-2C2G) prevented aggregation during 2 × CsCl gradient purification but in coxsackie and adenovirus receptor (CAR)-low/negative cancer cell lines of human and rodent origin, this reduced cytolytic efficacy to the levels observed for a fiber non-modified control vector. However, both Ad5-delta-24-RGD4C and Ad5-delta-24-RGD2C2G were equally effective in the murine immunocompetent CT-2A glioma model due to a primary role of antitumor immune responses in the therapeutic efficacy of oncolytic virotherapy.

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Section: Discussionmentioning

confidence: 99%

A new insight into aggregation of oncolytic adenovirus Ad5-delta-24-RGD during CsCl gradient ultracentrifugation

Stepanenko

Сосновцева

Valikhov

et al. 2021

Sci Rep

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“…Briefly, Potassium persulfate and ABTS stock solution were combined to produce the ABTS radical, which was then allowed to stand in the dark at room temperature for 12 to 16 h before use. 27 Then, the test solution was prepared by mixing a specific quantity of ABTS radical stock solution with 95% ethanol in a ratio of 1:15. The following concentrations of the extracts and positive controls were prepared; MBE (1, 2, 3, 4, and 5 µg/mL), MLE (20,40,60,80, and 100 µg/mL), Ascorbic acid and quercetin (1, 1.5, 2, 2.5, and 3 µg/mL).…”

Section: 2-azinobis (3-ethylbenzothiazoline-6-sulfonic Acid (Abts) Di...mentioning

confidence: 99%

Phytochemical Constituents, Anti-Diabetic and Antioxidant Activities of Methanol Extracts of Diospyros malabarica (Desr.) Kostel Leaves and Stem Bark

2024

TJNPR

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Therefore, there is the need for a therapeutic agent that will reduce glucose absorption by inhibiting the action of carbohydrate hydrolyzing enzymes such as α-glucosidase, 2,3 inhibiting the action of the DPP-4 enzyme which plays a role in reducing glucagon secretion and stimulating insulin secretion from the pancreas, 2 and scavenging free radicals. Diabetes mellitus and its complications are associated with increased oxidative stress caused by free radicals. 3 Free radicals are compounds with unpaired electrons which make them unstable and very reactive. In order to attain a paired electron state and become stable, free radicals oxidizes biomolecules and cell organelles, thus inducing cell damage. 4 Most free radicals in the body are reactive oxygen species (ROS) consisting of superoxide anion (O2•-), alkoxyl radical (RO•) radical, hydroxyl radical (OH•), peroxyl radical (ROO•), and hydroperoxyl radical (HO2•), while nitric oxide (NO•), nitrite (NO2•), and nitrate radicals (NO3•) are termed the reactive nitrogen species (RNS). 5 Free radical activity causes physiological disturbances in cells; therefore, antioxidants are needed to neutralize or mitigate free radical levels to maintain cell physiological functions and contribute to disease prevention. Antioxidant activity protects the body against the damaging effects of oxidative stress due to hyperglycemia and can improve carbohydrate, protein and lipid metabolism and glucose absorption in people with diabetes mellitus. 6 Currently, several types of drugs are available for the treatment of diabetes including non-insulin anti-diabetic drugs such as sulfonylureas, biguanides, thiazolidinediones, α-glucosidase inhibitors, Dipeptidyl peptidase 4 (DPP-4) inhibitors, and Sodium-glucose cotransporter-2 (SGLT2) inhibitors. 7 However, the use of anti-diabetic

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“…Consequently, the renal ability to infiltrate (glomerular filtration rate [GFR]) is decreased [7,8]. Reducing renal blood flow, in addition to reducing GFR, trigger the release of reactive oxygen species (ROS) through oxidative stress and osmotic necrosis induced directly by CM in tubular cells, although a direct renal oxidative stress action of CM has been challenged by in vitro studies [9]. As a result, acute necrosis of the renal tubules and renal hypoxia due to endothelial/tubular transport dysfunction are observed [[10], [11], [12]].…”

Section: Introductionmentioning

confidence: 99%

Contrast-induced nephropathy in an animal model: Evaluation of novel biomarkers in blood and tissue samples

et al. 2019

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An evaluation of the antioxidant properties of iodinated radiographic contrast media: An in vitro study

Abstract: Graphical abstract

Cited by 8 publications

References 34 publications

A new insight into aggregation of oncolytic adenovirus Ad5-delta-24-RGD during CsCl gradient ultracentrifugation

A new insight into aggregation of oncolytic adenovirus Ad5-delta-24-RGD during CsCl gradient ultracentrifugation

Phytochemical Constituents, Anti-Diabetic and Antioxidant Activities of Methanol Extracts of Diospyros malabarica (Desr.) Kostel Leaves and Stem Bark

Contrast-induced nephropathy in an animal model: Evaluation of novel biomarkers in blood and tissue samples

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